Laser confocal oblique incidence ellipsometry high-throughput biomolecular reaction imaging detection device

A laser confocal and biomolecular technology, applied in measurement devices, material excitation analysis, material analysis through optical means, etc., to achieve the effect of improving signal-to-noise ratio and detection sensitivity, simple device structure, and easy operation

Inactive Publication Date: 2011-11-30
TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no commercial product integrating laser c

Method used

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  • Laser confocal oblique incidence ellipsometry high-throughput biomolecular reaction imaging detection device
  • Laser confocal oblique incidence ellipsometry high-throughput biomolecular reaction imaging detection device
  • Laser confocal oblique incidence ellipsometry high-throughput biomolecular reaction imaging detection device

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Experimental program
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Effect test

Embodiment 1

[0038] See figure 1 , The laser confocal oblique incidence ellipsometric high-throughput biomolecular reaction imaging detection device includes a sample platform, a manual mechanical angle device, an ellipsometric detection unit, a confocal unit, and a data acquisition and data processing system.

[0039] A cover glass 16 carrying a biological sample microarray on the sample platform with a three-dimensional electric adjustment mechanism, and a multi-channel constant temperature flow cell 6 with a size of 15 mm × 15 mm × 3 mm under the cover glass, The multi-channel constant temperature flow cell 6 is made of quartz glass polytetrafluoroethylene material as the four walls and the bottom surface, and is sealed by a rubber sealing ring.

[0040] The liquid inlet of the multi-channel constant temperature flow cell 6 is connected with the liquid outlet of the multi-channel syringe pump 8 through the pipeline, and the liquid outlet of the multi-channel constant temperature flow ce...

Embodiment 2

[0053] The structure of the device is the same as in Embodiment 1, except that the silicon photodiode 12 adopts a photomultiplier tube of model 9863 / 100B, and others are the same as in Embodiment 1. The sensitivity and detection accuracy of the device in this embodiment can be improved by 2 orders of magnitude.

Embodiment 3

[0055]The structure of the device is the same as in Embodiment 1, except that the silicon photodiode 12 adopts a square high-speed induction silicon photodiode (BPX-65) with a photosensitive area of ​​1.0mm to form a 30 × 30 two-dimensional array, and the others are the same as in Embodiment 1. In this embodiment The device can realize the area of ​​9 (cm) 2 Microarray biological samples for detection.

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Abstract

The invention relates to a laser confocal obliquely-incident ellipsometric high-throughput biomolecular reaction imaging detection device which is characterized in that a sample substrate loaded with a biological sample microarray is arranged on a sample platform, and a multi-channel constant-temperature flow cell which is provided with a liquid inlet and a liquid outlet connected with a multi-channel injection pump is arranged below the sample substrate; the multi-channel constant-temperature flow cell is placed in the position of an objective table of an inverse laser confocal microscope; the two corresponding edges of the sample platform are respectively provided with two flat plates respectively provided with a groove, which are symmetrically and vertically arranged; the insides of the grooves are respectively provided with a mechanical arm, wherein one mechanical arm is provided with a laser device, a polarizer, a photoelastic modulator, a phase shifter and a focusing lens which are sequentially arranged in the direction of an incident light path, and the other mechanical arm is provided with a focusing lens, a chopper, a polarization analyzer and a photoelectric detector which are sequentially arranged in the direction of a reflected light path; and the data inlet/outlet ends of a phaselocked amplifier are respectively connected with the data outlet end of the photoelectric detector and the data inlet end of a data acquisition card, and a computer is respectively connected with the data acquisition card and a controller of the multi-channel injection pump.

Description

technical field [0001] The invention belongs to a device for high-throughput detection of biomolecular reactions, in particular to an oblique-incidence ellipsometric imaging detection device for real-time observation and high-throughput detection of biomolecular microarray (biochip) reactions based on a laser confocal microscope system. Background technique [0002] In life science research, the interaction between biomolecules is a basic life phenomenon, and it is also one of the major issues in modern life science research. There are many traditional methods for studying the interaction between biomolecules, such as radioimmunoassay Analysis methods, enzyme-linked immunoassay methods, labeling and tracing methods, etc. However, because these methods involve different types and contents of cells and biomolecules, and there are complex interactions between various substances, it is difficult to accurately obtain the relevant transmission information between biomolecules usin...

Claims

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Application Information

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IPC IPC(8): G01N21/17G01N21/64G01N33/571
Inventor 张洪艳汪鹏飞刘卫敏
Owner TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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