A kind of preparation method of dna or rna complex
A complex and cationic polymer technology, applied in the field of biomedical materials, can solve the problems of unfavorable DNA or RNA transfer, poor controllability of complex structure, large size difference, etc., and achieve strong repeatability of operation and reproducible size Strong control and uniform size effect
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Embodiment 1
[0054] Dilute the standard phosphate buffer solution 5 times to prepare 0.2×PBS buffer solution; dissolve the DNA in the buffer solution at a concentration of 5×10 -4 g / ml; Polylysine (PLL) was dissolved in buffer at a concentration of 1×10 -3 g / ml; using a capillary electrophoresis instrument to provide an electric field; inject DNA and PLL from both ends of the capillary successively with pressure. In the presence of an electric field, DNA flows to the positive electrode, and PLL flows to the negative electrode. DNA and PLL flow in the presence of an electric field Mix down to obtain DNA or RNA complexes. Fix the injection volume of DNA, adjust the voltage and the injection volume of PLL to obtain DNA or RNA complexes of different sizes.
[0055] An atomic force microscope AFM (NANOSCOPE IIIa, EV scanning head, tapping mode) was used to observe the structure of the complex prepared under the electric field. The size of the complex was uniform, and the size was about 100 nm....
Embodiment 2
[0057] Dilute the standard phosphate buffer solution 5 times to prepare 0.2×PBS buffer solution; dissolve RNA in the buffer solution at a concentration of 5×10 -5 g / ml; Polylysine (PLL) was dissolved in buffer at a concentration of 1×10 -4 g / ml; using a capillary electrophoresis apparatus to provide an electric field; inject RNA and PLL from both ends of the capillary successively with pressure. In the presence of an electric field, RNA flows to the positive pole, and PLL flows to the negative pole. RNA and PLL flow in the presence of an electric field. Mix down to obtain RNA complexes. Fix the injection volume of RNA, adjust the voltage and the injection volume of PLL, and obtain RNA complexes of different sizes.
[0058] An atomic force microscope AFM (NANOSCOPE IIIa, EV scanning head, tapping mode) was used to observe the structure of the complex prepared under the electric field. The size of the complex was uniform, and the size was about 100 nm.
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