A kind of anti-foot-and-mouth disease virus monoclonal antibody and use

Abstract

The invention relates to the technical field of virus immunology, in particular to a method for preparing an anti-O-type foot-and-mouth disease virus monoclonal antibody and the application of the prepared antibody, and belongs to the field of foot-and-mouth disease prevention and control. The microorganism preservation number of the hybridoma cell line 6B8 capable of specifically secreting monoclonal antibody against type O foot-and-mouth disease virus of the present invention is: CCTCC No: C201049. The subtype of monoclonal antibody produced by the hybridoma cell line hybridoma cell line 6B8 is IgG1. The hybridoma cell line of the invention can be used in preparing reagents for diagnosing type O foot-and-mouth disease. The monoclonal antibody of the invention can be used in the preparation of reagents for diagnosing type O foot-and-mouth disease.

Description

technical field [0001] The invention relates to the technical field of virus immunology, in particular to a preparation method of an anti-O-type foot-and-mouth disease virus monoclonal antibody and an application of the prepared antibody, belonging to the field of foot-and-mouth disease prevention and control. Background technique [0002] Foot-and-mouth disease is a severe infectious disease caused by foot-and-mouth disease virus that seriously harms cloven-hoofed animals. Because the disease can cause the death of young animals, the decline of animal production performance, the reduction of the quality and quantity of livestock products, and the rapid spread, once it occurs, it will cause huge economic losses to the affected countries and regions, and severely hit foreign trade. called "political economy disease". [0003] As a major animal disease worldwide, foot-and-mouth disease has always been an important target of quarantine, prevention, control and monitoring at po...

AI Technical Summary

Problems solved by technology

This is mainly because although monoclonal antibodies have many of the above advantages, they also have the disadvantages of long preparation period and unpredictable results.

In addition, the antigenic structure of FMD virus is extremely complex, and there are great differences in the antigenic structure of different serotypes, genotypes and isolates

These all limit the development of FMD monoclonal antibody

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