[0020] Example one
[0021] Material: H-60 (source: Nozaki Seed Plant in Japan, sold by Zhenjiang Ruifan Agronomic Co., Ltd.), Qiangli 50 (source: sold by Kobayashi Corporation of Japan and Wuhan Jiutou Bird Seed Industry Company).
[0022] For the process mode of this embodiment, see figure 1 , Which is specifically bred according to the following steps:
[0023] Step one, select the female parent. The H-60 cabbage was sown and cultivated in the early autumn of 1998, and the excellent single plants with good growth, round leaf bulbs and strong disease resistance were selected for retention, and the flowering period was carried out in the following spring. The seeds were obtained by selfing during the budding stage. The seeds obtained were sown in the autumn of 1999 and then planted in a greenhouse. A total of 42 plants were planted. The relative humidity in the shed was artificially controlled at 75%-85% and the temperature was 22℃-30℃. At the adult plant stage, select excellent individual plants with good growth, rounded leafballs and strong disease resistance from 42 plants and continue to reserve seeds. The following spring will carry out self-crossing at the flowering and bud stages to obtain seeds. The obtained seeds will be sown in the autumn of that year. Planted in the greenhouse to compare and identify the quality, select good individual plants and continue to reserve seeds. Such continuous self-purification and directional selection for multiple generations. After the basic traits of the third generation are stable, the quality will be determined and combined with the reserve for selfing Determination of incompatibility, selection of good quality, ie total sugar content ≥3.50%, VC content ≥410.0 mg·kg -1 , The crude protein content is ≥1.35%, the crude fiber content is ≤0.55%, and the self-compatibility index at the flowering stage is ≤1, and the individual plants with the self-compatibility index ≥5 at the bud stage will continue to self-purify and direct selection until the fifth The self-incompatible line 99-2-1-3-5 with good agronomic traits, strong disease resistance and stable self-incompatibility was selected from the generation, and the self-incompatible line 99-2-1- The spread of 3-5 plants is 52~54 cm, and the number of outer leaves is 14~15. It grows vigorously, has strong heat and humidity resistance, and is relatively disease-resistant. The leaf bulbs are oblate, neat, tight, and single bulb weight. About 1.15 kg, total sugar content 3.57%, VC 422.3 mg·kg -1 , Crude protein 1.39%, crude fiber 0.54%, neat appearance. The full growth period is about 60 days, and the self-compatibility index at flowering and bud stage is 0.35 and 9.6, respectively (for the meaning of self-purification and directional selection mentioned above, please refer to reference books in this field, which will not be described here; the above-mentioned agronomic traits refer to: Leaf number, spread, plant height, leaf bulb color, spherical shape, central column length and width, leaf bulb length and width, single bulb weight, compactness).
[0024] Step two, select the male parent. In the early autumn of 1998, the powerful 50 cabbage will be sown and cultivated, and excellent single plants with good growth, round leaf bulbs and strong disease resistance will be selected for retention. The flowering and budding will be carried out in the following spring. The seeds were obtained by self-crossing in the period. The seeds obtained were sown in autumn of 1999 and then planted in the greenhouse. A total of 55 plants were planted. The relative humidity in the greenhouse was artificially controlled at 75%-85% and the temperature was 22℃-30℃. At the adult plant stage, select the excellent single plants with good growth, round leaf balls and strong disease resistance from 55 plants to continue to reserve seeds. The following spring will be self-crossed at the flowering and bud stages to obtain seeds, and then the obtained seeds will be sown in the autumn of that year. Planted in the greenhouse to compare and identify the quality, select good individual plants and continue to reserve seeds. Such continuous self-purification and directional selection for multiple generations. After the basic traits of the third generation are stable, the quality will be determined and combined with the reserve for selfing Incompatibility determination, select the self-compatibility index at the flowering stage ≤ 1, and the self-compatibility index at the bud stage ≥ 5, and good quality (total sugar content ≥ 3.50%, VC content ≥ 410.0 mg·kg -1 The individual plants with crude protein content ≥1.35% and crude fiber content ≤0.55%) continue to self-purify and directional selection, until the fifth generation is selected to breed good agronomic traits, strong disease resistance, and stable self-incompatibility The self-incompatible line, named 02-7, as the male parent, the plant extension of the self-incompatible line 02-7 is 50~52 cm, the number of outer leaves is 12~13, it has strong stress resistance and resistance Virus disease, black rot, strong heat resistance, moisture resistance, not easy to crack, high yield, high oblate leaf bulb, average single bulb weight 1.1 kg, total sugar content 3.60%, VC 436.5 mg·kg -1 , Crude protein is 1.37%, crude fiber is 0.51%. The full growth period is 56 days. The self-compatibility index at flowering and bud stage were 0.48 and 11.9 respectively;
[0025] Step 3: Breed hybrids. In the spring of 2004, select stable and excellent cabbage self-incompatibility lines 99-2-1-3-5 and 02-7 to prepare hybrid combinations and test the combining ability. , The combination of both positive and negative crosses shows stress resistance, strong disease resistance, good quality and high yield.
[0026] Seed production of the above-mentioned hybrids according to the following methods:
[0027] Step 1: In the first ten days of August, plant the male and female parents 02-7 and 99-2-1-3-5 to raise seedlings;
[0028] Step 2: After the emergence of the seedlings, set up a shade shed to prevent rainstorm and scorching sun;
[0029] Step 3: When the seedlings have 7-8 leaves, the parents should be planted according to the row ratio of 1:1, and the total number of plants per mu is 2000±20;
[0030] Step 4. From the seedling stage to the flowering stage, strictly inspect and remove the miscellaneous and weak plants that do not meet the typical characteristics of the parent; (The above-mentioned non-conformity to the typical characteristics of the parent refers to: plant type, leaf shape, leaf color, leaf edge ripple, spherical shape , Flowering date is inconsistent with the parent);
[0031] Step 5: Put 2-3 boxes of bees per mu during the flowering period to increase the hybridization rate and hybrid seed yield.
[0032] Step 6. When 70% of the seed pods turn yellow and dry, they can be harvested when the morning dew is not dry. After ripening for 2 to 3 days, they are dried and threshed, and the removed seeds are dried in time and lifted clean.
[0033] The cabbage obtained above participated in the provincial variety identification test and was named Ruigan 60. This variety passed the provincial variety identification of Jiangsu Province in 2010. The certification number is Sujian cabbage 201002. Its main agronomic traits and characteristics are as follows:
[0034] Ruigan 60 plants have vigorous growth, strong heat and humidity resistance, strong stress resistance, resistance to viral diseases and black rot. The plant height is about 29 cm, the spread is about 51 cm, the number of outer leaves is 15-16, the leaf color is light green, obovate, the leaf bulb is high and oblate, the bulb is compact, green, crisp and sweet, and has high marketability , Crack resistant ball, single ball weight is about 1.3 kg, ball height is about 12.5 cm, transverse diameter is about 17.5 cm, center column is about 6.5 cm long, and center column is about 2.7 cm wide. The full growth period is about 94 days. 667m 2 The output reaches about 4000 kg.
[0035] In order to compare with the method of artificial inoculation identification, the two parental materials were used for artificial inoculation identification of three kinds of disease resistance to black rot, soft rot and root swelling at the seedling stage from 2003 to 2004. For the identification methods of artificial inoculation resistance of three kinds of cabbage diseases, please refer to References: 1. Zhang Enhui, Cheng Yongan, Xu Zhongmin, etc. Research on screening of three kinds of disease resistance and breeding of resistant varieties of cabbage. Journal of Northwest Sci-Tech University of Agriculture and Forestry, 2001, 29 ( 6): 30-33 1.1 Pathogen isolation and purification-1.2 Seedling resistance identification methods and procedures; 2. Si Jun, Li Chengqiong, Song Hongyuan, etc. Identification and evaluation of the resistance of cabbage to root swelling. Journal of Southwest University , 2009, 31(6): Method 1.2 in 26-31. The description is not expanded here. The results are shown in Table 1. It can be seen that the resistance of the two parent materials to black rot, soft rot and root swelling is strong, and the trend of artificial inoculation is consistent with the identification of disease resistance under the conditions of field greenhouse.
[0036] Table 1. Artificial inoculation of parental materials and identification of disease resistance in field greenhouses
[0037]
