A method for screening endophytic fungi that can improve the ability of crops to resist fungal diseases

An endophytic and antifungal technology, applied in the biological field, can solve the problems of unsuitable large-scale popularization and application, high cost and general effect.

Inactive Publication Date: 2011-12-21
XIAN UNVERSITY OF ARTS & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Scientists have also tried many non-pesticide control methods, such as using garlic, pepper and ginger to control black spot, chitosan to control downy mildew (Sun Tong, 2002), baking soda and potassium permanga...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039]Collect healthy rose stems with leaves, and use 75% ethanol (mass concentration) and 3.5% sodium hypochlorite (mass concentration) for surface disinfection in the laboratory, take the terminal buds of the branches, and cut about 2mm of the bud source base under the dissecting microscope. Then inoculated on MS semi-solid medium containing 3mg / L 6-BA and 0.1 mg / L NAA and cultivated. After forming clustered buds, cut off the buds that grew more than 2.5cm, and transfer them to the medium containing 0.5mg / L Cultivate on 1 / 2MS solid medium of IBA, and when the bottom of the buds form strong roots, the test-tube seedlings are domesticated and transplanted to the greenhouse for cultivation.

[0040] At the same time, cut the collected Chinese rose leafy stems into tissue blocks with a diameter of about 5 mm, and disinfect the surface of the plant tissue with 75% ethanol and 3.5% sodium hypochlorite, and then put the sterilized plant tissue blocks into a plate with wort juice sol...

Embodiment 2

[0044] Collect healthy wheat stems with leaves, disinfect the surface with 75% ethanol (mass concentration) and 3.5% sodium hypochlorite (mass concentration) in the laboratory, take the terminal buds of the branches, and cut about 2mm of the bud source base under the dissecting microscope. Then inoculated on MS semi-solid medium containing 3mg / L 6-BA and 0.2 mg / L NAA and cultured. After forming clustered buds, cut off the buds growing more than 2.5cm, and transfer them to the medium containing 0.5mg / L Cultivate on 1 / 2MS solid medium of IBA, and when the bottom of the buds form strong roots, the test-tube seedlings are domesticated and transplanted to the greenhouse for cultivation.

[0045] At the same time, the collected wheat stems with leaves were cut into tissue pieces with a diameter of about 5 mm, and the surface of the plant tissue was sterilized with 75% ethanol and 3.5% sodium hypochlorite, and then the sterilized plant tissue pieces were placed in a plate with wort ju...

Embodiment 3

[0049] Collect healthy snow lotus leaves, disinfect the surface with 75% ethanol (mass concentration) and 3.5% sodium hypochlorite (mass concentration) in the laboratory, cut the leaves into tissue pieces of about 0.5cm×0.5cm in a dissecting mirror, and then inoculate them on Culture on MS semi-solid medium with 3mg / L 6-BA and 0.2 mg / L NAA. After the formation of clustered buds, cut off the buds that grow more than 2.5cm, and transfer them to 1 / 1 of IBA containing 0.2mg / L. 2MS solid medium, when the bottom of the buds form strong roots, the test-tube seedlings are domesticated and transplanted to the greenhouse for cultivation.

[0050] Simultaneously, cut the collected snow lotus leafy stems into tissue blocks with a diameter of about 5 mm, carry out plant tissue surface disinfection with 75% ethanol and 3.5% sodium hypochlorite, and then put the sterilized plant tissue blocks into a plate with wort juice solid medium Cultivate (25 o C, natural light), observed regularly, an...

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PUM

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Abstract

The invention discloses a method for screening endophytic fungi capable of improving the ability of crops to resist fungal diseases. The steps include: 1) collecting plant materials to obtain plant-free transplanted seedlings without any disease; 2) separating endophytic fungi from plants fungi; 3) Prepare endophyte spore suspensions from the isolated endophytic fungi, and mycelial suspensions from endophytic fungi that do not produce spores; 4) Inoculate endophytic fungal spores or mycelial suspensions until the 5) Infect the transplanted seedlings inoculated with endophytic fungi and the transplanted seedlings not inoculated with endophytic fungi with pathogenic fungi, and observe the control after one month Effect, the endophytic fungi with a control effect greater than 80% are endophytic fungi that can improve the ability of the crops to resist fungal diseases.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for screening endophytic fungi capable of improving the ability of crops to resist fungal diseases. Background technique [0002] Crops refer to various plants cultivated in agriculture. Including food crops, economic crops (oil crops, vegetable crops), industrial raw material crops, feed crops, medicinal crops, etc., and thus are closely related to human food, clothing, housing and transportation. However, due to the large-scale cultivation of a single species of crops, it is easy to be infected with various plant diseases, mainly fungal diseases (Chen Hongbo, Wang Wei, 2009), such as gray mold, powdery mildew, downy mildew, branch blight , black spot and rust etc. Fungal diseases are very harmful. Among them, some fungal diseases have a high incidence rate, a wide range, and great harm. And because most of them can be spread by wind and rain and their mycelium or spores...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 徐玲玲陶贵荣郭斌付洪冰
Owner XIAN UNVERSITY OF ARTS & SCI
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