A method for screening endophytic fungi that can improve the ability of crops to resist fungal diseases
An endophytic and antifungal technology, applied in the biological field, can solve the problems of unsuitable large-scale popularization and application, high cost and general effect.
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Embodiment 1
[0039]Collect healthy rose stems with leaves, and use 75% ethanol (mass concentration) and 3.5% sodium hypochlorite (mass concentration) for surface disinfection in the laboratory, take the terminal buds of the branches, and cut about 2mm of the bud source base under the dissecting microscope. Then inoculated on MS semi-solid medium containing 3mg / L 6-BA and 0.1 mg / L NAA and cultivated. After forming clustered buds, cut off the buds that grew more than 2.5cm, and transfer them to the medium containing 0.5mg / L Cultivate on 1 / 2MS solid medium of IBA, and when the bottom of the buds form strong roots, the test-tube seedlings are domesticated and transplanted to the greenhouse for cultivation.
[0040] At the same time, cut the collected Chinese rose leafy stems into tissue blocks with a diameter of about 5 mm, and disinfect the surface of the plant tissue with 75% ethanol and 3.5% sodium hypochlorite, and then put the sterilized plant tissue blocks into a plate with wort juice sol...
Embodiment 2
[0044] Collect healthy wheat stems with leaves, disinfect the surface with 75% ethanol (mass concentration) and 3.5% sodium hypochlorite (mass concentration) in the laboratory, take the terminal buds of the branches, and cut about 2mm of the bud source base under the dissecting microscope. Then inoculated on MS semi-solid medium containing 3mg / L 6-BA and 0.2 mg / L NAA and cultured. After forming clustered buds, cut off the buds growing more than 2.5cm, and transfer them to the medium containing 0.5mg / L Cultivate on 1 / 2MS solid medium of IBA, and when the bottom of the buds form strong roots, the test-tube seedlings are domesticated and transplanted to the greenhouse for cultivation.
[0045] At the same time, the collected wheat stems with leaves were cut into tissue pieces with a diameter of about 5 mm, and the surface of the plant tissue was sterilized with 75% ethanol and 3.5% sodium hypochlorite, and then the sterilized plant tissue pieces were placed in a plate with wort ju...
Embodiment 3
[0049] Collect healthy snow lotus leaves, disinfect the surface with 75% ethanol (mass concentration) and 3.5% sodium hypochlorite (mass concentration) in the laboratory, cut the leaves into tissue pieces of about 0.5cm×0.5cm in a dissecting mirror, and then inoculate them on Culture on MS semi-solid medium with 3mg / L 6-BA and 0.2 mg / L NAA. After the formation of clustered buds, cut off the buds that grow more than 2.5cm, and transfer them to 1 / 1 of IBA containing 0.2mg / L. 2MS solid medium, when the bottom of the buds form strong roots, the test-tube seedlings are domesticated and transplanted to the greenhouse for cultivation.
[0050] Simultaneously, cut the collected snow lotus leafy stems into tissue blocks with a diameter of about 5 mm, carry out plant tissue surface disinfection with 75% ethanol and 3.5% sodium hypochlorite, and then put the sterilized plant tissue blocks into a plate with wort juice solid medium Cultivate (25 o C, natural light), observed regularly, an...
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