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A class of fluorescent molecular imaging probes and magnetic/fluorescent dual-mode molecular imaging probes and preparation methods thereof

A technology of fluorescent molecules and molecular imaging, applied in the direction of nuclear magnetic resonance/magnetic resonance imaging contrast agent, etc., to achieve the effect of small particle size distribution, convenient universality and good relaxation performance

Active Publication Date: 2011-12-21
INST OF CHEM CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the embedded metallofullerene contrast agents and other MRI contrast agents obtained by traditional modification methods are all native single-modal contrast agents, which need to be further modified by luminescent groups or nuclides to obtain combined magnetic / fluorescent or magnetic / PET Isobimodal Molecular Imaging Probes for Multiplex Detection

Method used

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  • A class of fluorescent molecular imaging probes and magnetic/fluorescent dual-mode molecular imaging probes and preparation methods thereof
  • A class of fluorescent molecular imaging probes and magnetic/fluorescent dual-mode molecular imaging probes and preparation methods thereof
  • A class of fluorescent molecular imaging probes and magnetic/fluorescent dual-mode molecular imaging probes and preparation methods thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1, preparation based on metal fullerene GdC 82 Dual-modal (magnetic / fluorescent) integrated molecular imaging probe

[0047] (a) The embedded metallofullerene GdC82 can be prepared according to the following references: Shu, C.Y., et al., Carbon, 2006.44(3): p.496-500. Cagle.D.W., PNAS, 1999.96: p.5182- 5187.

[0048] The details are as follows: GdC containing metal fullerenes was prepared by DC arc discharge method. 82 The ashes, extracted with toluene (DMF or o-dichlorobenzene) combined with high performance liquid chromatography (using fullerene / embedded metal fullerene special chromatographic column Buckyprep / Buckyprep-M) separation and purification to obtain high-purity metal fullerene ene GdC 82 The toluene solution was evaporated to dryness to obtain a black solid (A).

[0049] (b) prepare the mixed solution of 20mL mass concentration 30% hydrogen peroxide solution and mass concentration 28% ammoniacal liquor (V 过 氧化氢 :V 氨水 =7:3) (B).

[0050] ...

Embodiment 2

[0065] Embodiment 2, preparation based on hollow fullerene C 60 Fluorescent Molecular Imaging Probes

[0066] With purified 100mg C 60 Solid and 70mL mass concentration 30% hydrogen peroxide solution / mass concentration 28% ammonia water mixed solution (V 过氧化氢 :V 氨水 =7:3), stirred at 60°C for 12h, and the remaining conditions were the same as in Example 1. Based on the hollow fullerene C 60 fluorescent molecular imaging probes. Figure 12 Illustrate, the hollow fullerene C of the inventive method modification 60 Based on the metal fullerene GdC prepared in Example 1 82The bimodal (magnetic / fluorescent) integrated molecular imaging probe has similar fluorescence spectra.

Embodiment 3

[0067] Example 3. Preparation of graphite oxide-based fluorescent molecular imaging probes

[0068] With 5mg of purified graphene oxide aqueous solution (1.67mg / mL) and 30mL mass concentration 30% hydrogen peroxide solution / mass concentration 28% ammonia water mixed solution (V hydrogen peroxide: V ammonia water=3:1), 85 ℃ Under stirring for 16h, the rest of the conditions are the same as in Example 1.

[0069] Graphite oxide-based fluorescent molecular imaging probes. Figure 13 Illustrate, the graphene oxide modified by the inventive method and the metallofullerene-based GdC prepared in Example 1 82 The bimodal (magnetic / fluorescent) integrated molecular imaging probe has similar fluorescence spectra.

[0070] Table 1

[0071]

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Abstract

The invention discloses a novel bimodal (magnetic / fluorescent) integrated molecular image probe and a preparation method. The preparation method for the molecular image probe comprises the following steps of: adding a carbon nanometer material into mixed solution of hydrogen peroxide and ammonia water, and reacting to obtain solution containing the magnetic / fluorescent bimodal molecular image probe; centrifuging to remove insoluble substances and concentrating; and adding ethanol into concentrated solution, oscillating to generate a large number of precipitates, collecting the precipitates, washing by using the ethanol, and drying to obtain the magnetic / fluorescent bimodal molecular image probe, wherein the carbon nanometer material may be one of metal-embedded fullerene, hollow fullereneand oxidized graphene. In the method, the intervention of an organic solvent is saved, intermediate products are not needed to be separated, conditions of water, oxygen and the like are saved, and the preparation method has the advantages of simplicity, economy, environment friendliness and the like.

Description

technical field [0001] The invention relates to a class of fluorescent molecular image probes and magnetic / fluorescent dual-mode molecular image probes and a preparation method thereof. Background technique [0002] Molecular imaging technology can realize in vivo, real-time, dynamic, and non-invasive three-dimensional imaging of physiological and pathological changes of biological organisms at the molecular level, and the dual and multi-modal technology that integrates different images can realize the complementary advantages of different imaging devices, thereby reducing False positives and false negatives make the diagnostic results obtained more accurate. The construction of multimodal molecular probes is an important way to effectively improve the imaging quality and the accuracy of tumor diagnosis. Dual and multi-modal fusion has become the development trend of biomedical imaging. The intervention of multi-modal molecular probes has transformed molecular imaging from ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/06
Inventor 王春儒郑俊鹏舒春英甄明明
Owner INST OF CHEM CHINESE ACAD OF SCI
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