Preparation method of natamycin flaky crystal

A technology for natamycin and flaky crystals, applied in the field of preparing natamycin flaky crystals, can solve the problems of affecting purity, increasing the difficulty of filtering, washing and drying, unfavorable filtering and drying of natamycin crystals, etc. , to facilitate production control, shorten filtration and drying time, and reduce difficulty

Active Publication Date: 2011-12-21
LIFECOME BIOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The needle-like crystal habit is easy to coalesce into clusters, and when coalescing, it is easy to wrap impurities and affect the purity, and at the same time increases the difficulty of filtration, washing and drying
The natamycin crystals obtained by the extraction method announced by Chinese patent CN101062934A are short needle-shaped or amorphous, which is not conducive to the filtration and drying of the natamycin crystals, and the filtration and drying time are all longer

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  • Preparation method of natamycin flaky crystal
  • Preparation method of natamycin flaky crystal
  • Preparation method of natamycin flaky crystal

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specific Embodiment approach

[0022] The specific embodiment of the present invention provides a kind of preparation method of natamycin lamellar crystal, it is characterized in that: it comprises the following steps:

[0023] a. Get natamycin mycelium and add it to the mixed solution of methanol and water; in the mixed solution of methanol and water, the volume ratio of methanol to water is 60-90:40-10; every 1L of methanol and water Add 90-110g natamycin mycelia to the mixed solution;

[0024] The natamycin mycelium described in this step is the wet mycelium or dry mycelium obtained from the natamycin fermentation broth through liquid-solid separation technology, preferably dry mycelium with a moisture content of ≤5%. Of course, wet mycelia can also be used. If the water content of the wet mycelium is high, it is best to measure the water content of the wet mycelium in advance, so that the water content can be appropriately reduced when preparing the mixed solution of methanol and water. proportion. Th...

Embodiment 1

[0038]Get 100 g of dried mycelium of natamycin, wherein the content of natamycin is 11.2% (w / w), add 1L of methanol and water mixture (volume ratio of methanol to water is 75:25), stir to make The mycelium is well suspended. Adjust the pH value to 11.35 with 10 mol / L NaOH and stir for 1 hour. The bacterial residue was separated by suction filtration, and the filtrate was collected to obtain a yellow-brown natamycin extract. Concentrate the natamycin extract until the content of natamycin is 22.6mg / ml, and flocculate to remove the insoluble matter precipitated in the process, to obtain the fine extract of natamycin. Then slowly add 10 mol / L HCl dropwise while stirring, so that the pH value of the natamycin fine extract gradually decreases to 8.65, and then stir for 1 hour when the pH value does not rise again. Then continue to slowly add 10 mol / L of HCl dropwise while stirring, so that the pH value of the natamycin essence extract is gradually reduced to 6.61, and then stir f...

Embodiment 2

[0040] Get the dried mycelium 90g of natamycin, wherein the content of natamycin is 12.1％ (w / w), add 1L methanol and water mixture (the volume ratio of methanol and water is 60:40), stir to fully suspend the mycelium. Adjust the pH value to 10 with 10 mol / L NaOH and stir for 1.5 hours. The bacterial residue was separated by suction filtration, and the filtrate was collected to obtain a yellow-brown natamycin extract. Concentrating the natamycin extract until the content of natamycin is 18 mg / ml, and flocculating to remove the insoluble matter precipitated in the process, to obtain the fine extract of natamycin. Then slowly add 10 mol / L HCl dropwise while stirring, so that the pH value of the natamycin fine extract is gradually reduced to 8, and then stir for another 1.5 hours when the pH value does not rise again. Then continue to slowly add 10 mol / L of HCl dropwise while stirring, so that the pH value of the natamycin fine extract is gradually reduced to 6, and then stir fo...

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Abstract

The invention relates to a preparation method of a natamycin laminar crystal, and the preparation method comprises the following steps: (1) adding natamycin mycelium into a methanol and water mixed liquid, wherein the volume ratio of methanol to water is (60-90):(40-10); (2) adjusting the pH value to 10-12 with a basic substance; (3) carrying out liquid-solid separation and collecting filtrate to obtain a natamycin extract; (4) concentrating the extract until the content of natamycin is 18-26 mg / mL, and flocculating to remove insoluble substances to obtain a natamycin fine extract; (5) acidifying the natamycin fine extract by two stages: firstly dripping acid slowly until the pH value decreases to 8-9, then stirring for more than 1 hour, then slowly dripping the acid until the pH value decreases to 6-7, and then stirring for more than 3 hours to obtain a crystallization liquid of the laminar natamycin crystal; and (6) carrying out liquid-solid separation and drying in vacuum to obtain the laminar natamycin crystal. By adoption of the preparation method provided by the invention, the uniform natamycin laminar crystal can be prepared, the subsequent crystal washing and drying difficulties are reduced, and the crystal filtering and drying time is shortened.

Description

technical field [0001] The invention relates to a method for preparing natamycin flake crystals from mycelia. Background technique [0002] Natamycin, also known as Pimaricin, is a polyene macrolide antibiotic, which can inhibit the growth of yeast and mold, and does not affect the normal flora in the human intestinal tract. Since 1996, the my country Food Additives Committee has carried out follow-up research and evaluation on the application of natamycin. In 2011, the national standard for natamycin was officially issued. It is used as a food anti-mold agent in cheese, pastry, and sausage. During food processing. [0003] The molecular formula of natamycin is C33H47NO13; the molecular weight is 665.75 Daltons; it is an amphoteric compound with an isoelectric point of 6.5. [0004] Natamycin is produced by Streptomyces fermentation. After liquid-solid separation of the fermentation broth, the mycelium is collected, and the crystals of natamycin can be finally obtained by a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/08C07H1/06
Inventor 李俊辉姜献民赖潭平
Owner LIFECOME BIOCHEM
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