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Application of isoflavone compound to preparation of antitumor medicines or food

A compound and isoflavone technology, applied in the application field of preparing anti-tumor drugs or food, can solve the problems of no anti-tumor effect and the like

Inactive Publication Date: 2012-08-29
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] But so far, there is no report on the anti-tumor effect of irisin, irisaxanthin, subirisanthin, irisin or irisin

Method used

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  • Application of isoflavone compound to preparation of antitumor medicines or food
  • Application of isoflavone compound to preparation of antitumor medicines or food
  • Application of isoflavone compound to preparation of antitumor medicines or food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1. Preparation of irisin, irisaxanthin, subirisanthin, irisin, iridin (see Jin Li (Jin Li), Chen Hai-sheng (Chen Haisheng), et al.Chemical constituents from Belamcanda chinensis Journal of Asian Natural Products Research 2008, 10(1): 89-91)

[0020] 4Kg of dried rhizomes of traditional Chinese medicine were crushed and extracted three times under reflux with 80% ethanol, and the solvent was recovered under reduced pressure to obtain about 200g of liquid extract, which was dispersed in 800ml of distilled water and extracted with petroleum ether, chloroform and ethyl acetate in sequence 3 times (500ml each time), after recovering the solvent respectively, 22g of petroleum ether extract, 65g of chloroform extract, and 28g of ethyl acetate extract were obtained.

[0021] Get chloroform extract 65g and carry out silica gel column chromatography, use gradient solvent system CHCl 3 →CHCl 3 / CH 3 OH:H 2 O (20:1) eluted to give fractions C1, C2 and C3, respectively. ...

Embodiment 2

[0023] Example 2: Anti-tumor activity experiment of irisin, irisaxanthin, subirisanthin, irisin and irisin in vitro

[0024] 1. Experimental drug:

[0025] Irisgenin (BC-1), irisaxanthin (BC-2), second irisaxanthin (BC-3), irisin (BC-4), irisin (BC-5), by the embodiment 1 preparation;

[0026] Positive control drug: doxycycline (DOX), Changzhou Pharmaceutical Factory;

[0027] 2. Cell line: provided by the Pharmacology Laboratory of Shanghai Institute of Pharmaceutical Industry

[0028] ①A549 (human lung cancer cells)

[0029] ② LOVO (human intestinal cancer cells)

[0030] ③6T-CEM (human T-cell leukemia cells)

[0031] ④MDA-MB-435 (human breast cancer cells)

[0032] 3. Culture medium: RPMI1640+15%NBS+double antibody (10000U / mL penicillin+10000U / mL streptomycin)

[0033] 4. Other materials

[0034] Fully automatic microplate reader: Model: (WellscanMK-2) Manufacturer: 96-well cell culture plate from Finnish Labsystems (Nune, USA)

[0035] 5. Experimental method

[0...

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Abstract

The invention relates to the technical field of medicines and also relates to application of an isoflavone compound irigenin of Belamcanda chinensis (L.) DC. to preparation of antitumor medicines or food. According to an in vitro test, the isoflavone compound has the effect of obviously inhibiting cell growth of cell strains A549 (human lung cancer cells), LOVO (human intestinal cancer cells), 6T-CEM (human T cell leukemia cells) and HL-60 (human leukemia cells), so the isoflavone compound can be used for preparing the antitumor medicines or food. The invention provides a new source for preparing the antitumor medicines or food.

Description

technical field [0001] The invention relates to the technical field of medicine, and is the preparation of antitumor drugs or foods by the isoflavone compounds irisaxanthin, irisinin, subirisin, irisin or irisin in Shegan Belamcanda chinensis (L.) DC. in the application. This application is a divisional application of the Chinese patent application with application number 201010144912.8. Background technique [0002] Shegan is the dry rhizome of Belamcanda chinensis (L.) DC. The reported main components of Shegan are 19 isoflavone compounds such as irisgenin, irisxanthin, subirisin, irisflavone neoaglycone A, white sheganin, irisin and irisin[Jin Li (Jin Li), Chen Hai-Sheng (陈海生) et al.New flavone and isoflavone glycoside from Belamcanda chinensis, Chinese Chemical Letters 2007, 18: 158-160; Jin Li (金丽), Chen Hai-sheng (陈海生), et al. Chemical constituents from Belamcanda chinensis Journal of Asian Natural Products Research 2008, 10(1): 89-91; Hu Xiaolan, et al. Isolation ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7048A61P35/00A23L1/30
Inventor 陈海生金丽刘建国金永生赵卫权
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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