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A kind of total phenolic acid of Phyllostachys pulmonica and preparation method thereof

A kind of technology of A. pulmonica and total phenolic acid, which is applied in the field of pharmacy, can solve the problems that total phenolic acid compounds have not been reported in separate research, and achieve the effect of ensuring quality, curative effect and stability

Active Publication Date: 2011-12-28
沈阳双鼎制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the research on this drug has made great progress, but most of them focus on flavonoids, and there is no report on the separate research on the total phenolic acid compounds of another effective part

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A kind of preparation method of total phenolic acid of Chickweed, comprising the following process steps:

[0016] 1. Grind the medicinal material of Pasternia bruceae into 10cm long sections, add 0.5% sodium bicarbonate solution as solvent for percolation, collect 10 times the percolation liquid, adjust the pH value to 2.0 with dilute sulfuric acid, and filter. Precipitation standby;

[0017] 2. Pass the filtrate obtained in step 1 through a polyamide column, first wash with 5 times the amount of water to remove impurities, then elute with 90% ethanol, collect 8 times the amount of ethanol eluate, and set aside;

[0018] 3. Extract the precipitate obtained in step 1 with 5 times the amount of 90% ethanol for 3 times, filter, combine the filtrate with the ethanol liquid obtained in step 2, recover the ethanol at 50°C, concentrate under reduced pressure, add 4% sodium hydroxide solution to dissolve, Filtrate and dry to obtain 108 g of total phenolic acids of Paleopsis s...

Embodiment 2

[0020] A kind of preparation method of total phenolic acid of Chickweed, comprising the following process steps:

[0021] 1. Grind the medicinal material of Pasternia bruceae into 12cm long pieces, then add 0.3% sodium bicarbonate solution as a solvent for percolation, collect 12 times the percolation liquid, adjust the pH value to 2.5 with dilute sulfuric acid, and filter. Precipitation standby;

[0022] 2. Pass the filtrate obtained in step 1 through a polyamide column, first wash with 8 times the amount of water to remove impurities, then elute with 90% ethanol, collect 10 times the amount of ethanol eluate, and set aside;

[0023] 3. Extract the precipitate obtained in step 1 with 8 times the amount of 90% ethanol for 3 times, filter, combine the filtrate with the ethanol liquid obtained in step 2, recover the ethanol at 55°C, concentrate under reduced pressure, add 4% sodium hydroxide solution to dissolve, Filtrate and dry to obtain 113g of total phenolic acids of Paleop...

Embodiment 3

[0025] A kind of preparation method of total phenolic acid of Chickweed, comprising the following process steps:

[0026] 1. Grind the medicinal material of Pasternum spp. into 15cm long sections, add 0.2% sodium bicarbonate solution as solvent for percolation, collect 15 times the percolation liquid, adjust the pH value to 3 with dilute sulfuric acid, and filter. Precipitation standby;

[0027] 2. Pass the filtrate obtained in step 1 through a polyamide column, first wash with 10 times the amount of water to remove impurities, then elute with 90% ethanol, collect 15 times the amount of ethanol eluate, and set aside;

[0028] 3. Extract the precipitate obtained in step 1 with 10 times the amount of 90% ethanol for 3 times, filter, combine the filtrate with the ethanol liquid obtained in step 2, recover the ethanol at 60°C, concentrate under reduced pressure, add 4% sodium hydroxide solution to dissolve, Filtrate and dry to obtain 115 g of total phenolic acids of Paleopsis cum...

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Abstract

The invention relates to a preparation method for the total phenolic acid of ixeris sonchifolia (Bunge) hance. The preparation method comprises the following steps: crushing the medicinal material of the ixeris sonchifolia (Bunge) hance; adding 0.1-0.5% of a sodium bicarbonate solution adopted as a solvent to carry out percolation; collecting the percolation solution, wherein the volume of the percolation solution is 5-15 times the total volume of the ixeris sonchifolia (Bunge) hance and the sodium bicarbonate solution; adopting dilute sulfuric acid to adjust a pH value to 2-3; carrying out filtration and precipitation to provide a spare use; carrying out an elution treatment for the resulting filtrate through a polyamide column, firstly adopting the water to remove impurities, followed by adopting 90% ethanol to carry out the elution and collecting the ethanol eluent to provide a spare use, wherein the volume of the water is 1-5 times the total volume of the polyamide column, the volume of the collected ethanol eluent is 5-15 times the total volume of the polyamide column; adopting the 90% ethanol to carry out extraction for the precipitation for three times, wherein the volume of the 90% ethanol is 2-10 times the volume of the precipitation; then carrying out filtering, mixing the resulting filtrate and the collected ethanol eluent; recovering the ethanol at a temperature of40-70 DEG C; carrying out vacuum concentration; adding a 4% sodium hydroxide solution to dissolve, then carrying out steps of filtering and drying to obtain the total phenolic acid of the ixeris sonchifolia (Bunge) hance. In the prior art, the phenolic acid compounds are easy to be deposed and deteriorated during the preparation process and the storage process. With the total phenolic acid injection of the ixeris sonchifolia (Bunge) hance provided by the present invention, the disadvantages in the prior art are overcome, the stabilities of the active ingredients are ensured, such that the quality and the efficacy of the total phenolic acid injection of the ixeris sonchifolia (Bunge) hance are ensured.

Description

technical field [0001] The present invention relates to a total phenolic acid of P. pulmonica and a preparation method thereof. The invention also relates to a freeze-dried powder injection containing the total phenolic acid of P. chrysanthemum and a preparation method and a quality control method thereof, which belong to the field of pharmacy. Background technique [0002] Ixeris sonchifolia, commonly known as Kudiezi, Gypsophila, etc., is the biennial dry aboveground part of Ixeris sonchifolia (Bge) Hance in the family Asteraceae. Efficacy, clinical application is more extensive with Kudiezi injection. Kudiezi Injection is made from the medicinal herbs of Kudiezi, with main ingredients including flavonoids, phenolic acids, adenosine, amino acids, etc. It is used to treat ischemic cardiovascular and cerebrovascular diseases such as coronary heart disease, angina pectoris, and myocardial infarction. In recent years, the research on this drug has made great progress, but mos...

Claims

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Application Information

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IPC IPC(8): A61K36/28A61P7/02
Inventor 马占芝生可心吕志会王淞淋王学东
Owner 沈阳双鼎制药有限公司