A kind of method of fermenting citric acid
A citric acid and fermenter technology, which is applied in the field of fermenting citric acid, can solve the problems of inhibiting the activity of Aspergillus niger and synthesizing citric acid, and achieves the effect of avoiding activity reduction
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[0045] 2) The preparation method of seed culture solution: put 28m into the seed tank 3 After the enzymatic hydrolysis solution of corn flour in step 1), the seed medium of the seed tank was sterilized at 121° C. for 30 minutes, cooled to 36° C. and inserted into Aspergillus niger seeds, and the inoculation amount was 2×10 per gram of the enzymatic hydrolysis solution. 5 colony-forming units were cultured for 27 hours at a temperature of 36° C., a ventilation rate of 0.3 vol: (volume minutes), and a tank pressure of 0.1 MPa to obtain a seed culture solution.
[0046] 3) The preparation method of the fermentation medium: put the corn flour enzymolysis solution in the step 1) into the fermenter for 20m 3 , the remaining 210m 3 200m obtained after filtering 3 The enzymolysis clear liquid was put into the fermenter, and then 37.5kg of urea was added. After sterilizing at 80° C. for 10 minutes, the temperature was lowered to 37° C. to obtain a fermentation medium.
[0047] Stir...
Embodiment 1
[0050] This embodiment adopts 5 groups of fermentation tanks arranged in sequence, each group of fermentation tanks is composed of a seed tank, and the 5 groups of fermentation tanks are respectively numbered as the first fermentation tank, the second fermentation tank, the third fermentation tank, the fourth fermentation tank and Fifth fermenter. The time when the seed culture solution was introduced into the first fermenter to start fermentation was recorded as the start time.
[0051] Under agitation, under the ventilation rate of 0.12 volume: (volume minutes), the seed culture solution (that is, the first citric acid fermentation seed) is inserted into the first fermenter, and the insertion volume is: relative to each milliliter of fermentation The fermentation medium obtained in the culture medium was 1.0×10 4 colony forming unit, at a temperature of 35°C, an initial pH value of 4.3, and an aeration rate of 0.08 volume: (volume minutes), the first fermentation was carrie...
Embodiment 2
[0055] Adopt the method of embodiment 1 to carry out, and difference is: the access amount that inserts seed culture fluid (that is described first citric acid fermentation seed) in the first fermentor is: relative to every milliliter of fermented medium obtains The fermentation medium is 2.5×10 4 colony forming unit, the time of the first fermentation culture in the first fermenter is 30 hours, the amount of thalline residue inserted in the first fermenter is 7.5% by weight of the weight of the fermentation medium, and the bacterium Body residue is 4 minutes from the beginning of solid-liquid separation to the time interval of inserting into fermentor; 6 hours after the end of the first fermentation in the last fermenter. Wherein, the time of inserting the first citric acid fermentation seed, the time of inserting the second citric acid fermentation seed and the end time of fermentation are shown in Table 1; Table 2 shows.
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