A biosensing electrode for detecting atrazine and its manufacturing method

A technology of biosensing and atrazine, which is applied in the field of nano-modified electrodes and biological detection, can solve the problems of highly unstable enzymes, hindering enzyme reactions, and polluting electrodes, and achieves simple, highly ordered, and low-cost preparation processes. cheap effect

Inactive Publication Date: 2011-12-28
TONGJI UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, due to the high instability of the enzyme and the easy formation of intermediate products that hinder the enzyme reaction and contaminate the electrode, further improving the sensitivity has become the focus of most researchers.

Method used

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  • A biosensing electrode for detecting atrazine and its manufacturing method
  • A biosensing electrode for detecting atrazine and its manufacturing method
  • A biosensing electrode for detecting atrazine and its manufacturing method

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Embodiment

[0020] An ultra-high sensitive atrazine biosensing electrode, the sensing electrode uses a titanium plate as a base electrode, erect and ordered titanium dioxide nanotubes grown on the surface of the titanium plate, and tyrosinase modified in the tube and on the surface. The specific production process is as follows:

[0021] Use 100 # , 300 # and 500 # Sanding, and then further polishing with metallographic sandpaper to smooth the surface of the substrate into a mirror surface, followed by ultrasonic cleaning in distilled water and acetone for 15 minutes each. After cleaning with double distilled water, the titanium plate was etched in 10wt% oxalic acid for 1.5 hours, and then the titanium plate was cleaned with double distilled water and dried. At room temperature, the pretreated titanium sheet is used as the anode, and the platinum sheet electrode is used as the counter electrode, and the hydrofluoric acid of wt. ), a mixed solution of ammonium fluoride (0.25%) and ethy...

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PUM

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Abstract

The invention relates to a biological sensing electrode for detecting atrazine and manufacturing method thereof. The biological sensing electrode is composed of a titanium plate, vertical and orderly titanium dioxide nanotubes grown on the surface of the titanium plate through anode oxidation method, and modified tyrosinase in the tubes and on the surfaces thereof; a titanium dioxide nanotube electrode (TiO2-NTs) on the surface of the titanium plate is manufactured from the vertical and orderly titanium dioxide nanotubes grown on the surface of the titanium plate through anode oxidation method; and then the titanium dioxide nanotube electrode is pre-treated at constant potential of -1.5 V in 2 mol / L NH4Cl solution for 3 seconds; Tyr / TiO2-NTs is prepared by getting 20 muL 50U.muL-1 tyrosinase solution and 5 muL 2.5% of glutaral pentanedial with a micro-injector to cover on the surface of the electrode so as to prepare Tyr / TiO2-NTs. In comparison with existing technology, the manufacturing method of the invention is featured by simple process, low cost and good repeatability; when used for detecting low-concentration atrazine in the environment, detection stability is high; detection range is 0.2 ppt to 10 ppm; and limit of detection is 0.1 ppt.

Description

technical field [0001] The invention relates to a nano-modified electrode and the technical field of biological detection, in particular to a biosensing electrode for detecting atrazine and a manufacturing method thereof. Background technique [0002] Since atrazine was developed and produced by Swiss Ciba-Geigy Chemical Company in 1952, due to its high efficiency and other characteristics, it has become one of the most widely used herbicides in the world. According to studies, atrazine is an endocrine disruptor, and it may cause subacute damage to the body at low concentrations. In recent years, atrazine residues have been detected in groundwater and surface water in many regions. Therefore, there is an urgent need to establish a rapid and highly sensitive method for detecting low-concentration atrazine. [0003] Although traditional atrazine analysis methods (such as thin-layer chromatography, gas chromatography, liquid chromatography, and gas chromatography-mass spectro...

Claims

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Application Information

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IPC IPC(8): G01N27/327C25D11/26
Inventor 赵国华余志敏刘梅川张重宁吴涛
Owner TONGJI UNIV
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