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Combined inactivated vaccine of Newcastle disease and H9 subtype avian influenza and preparation method thereof

An inactivated vaccine, chicken Newcastle disease technology, applied in pharmaceutical formulations, medical preparations containing active ingredients, virus antigen components, etc., can solve the problems of difficult vaccination, large immune dose, large immune stress, etc. Long-term, small immune dose, and the effect of reducing stress response

Active Publication Date: 2012-01-04
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the dose of the dual vaccine is too large, 0.3ml per chicken
In this case, the immunization dose is large and the immune stress is large, especially not suitable for young chickens
Moreover, due to the cohesiveness of the oil emulsion vaccine, it brings difficulties to the vaccination of the vaccine.

Method used

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  • Combined inactivated vaccine of Newcastle disease and H9 subtype avian influenza and preparation method thereof
  • Combined inactivated vaccine of Newcastle disease and H9 subtype avian influenza and preparation method thereof
  • Combined inactivated vaccine of Newcastle disease and H9 subtype avian influenza and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The preparation of embodiment 1 chicken Newcastle disease and H9 subtype avian influenza double concentrated inactivated vaccine

[0020] 1 Preparation of poisonous seeds for production

[0021] 1. Propagation and identification of virus species of 1H9HL strain

[0022] 1.1.1 Breeding of virus seeds Avian influenza virus A / Chicken / Henan / Luoyang / HL / 2001 (H9N2) (abbreviated as HL strain). The HL strain freeze-dried virus was properly diluted with sterilized physiological saline (10 -3 ~10 -4 ), 0.1ml per allantoic cavity was inoculated with 10-day-old SPF embryos, and incubated at 37°C for 96 hours. During this period, the dead embryos before 48 hours were discarded. After 48 hours, the embryos were photographed once every 4 to 6 hours, and the dead embryos were removed in time. After 96 hours, take them all out, put them in the refrigerator at 2-8°C for more than 12 hours, harvest the chicken embryo fluids separately, put them into sterilized containers, mix the chick...

Embodiment 2

[0060] Embodiment 2, the effect measurement of chicken Newcastle disease and H9 subtype avian influenza dual concentrated inactivated vaccine

[0061] 1 physical properties

[0062] Appearance The finished preparation is a white homogeneous emulsion when observed with the naked eye.

[0063] The formulation is water-in-oil. Take a clean straw, suck a small amount of vaccine and drop it on the surface of cold water, in the shape of oil droplets, without spreading.

[0064] Stability Put the vaccine in a 10ml centrifuge tube, centrifuge at 3000rpm for 15min, and the water phase ≤0.5ml will precipitate out at the bottom of the tube.

[0065] Viscosity Use a 1ml straw with an outlet diameter of 1.2mm to draw 1.0ml of vaccine at about 25°C, let it flow out vertically and naturally, record the time required for the outflow of 0.4ml, within 8s, the inspection is qualified.

[0066] 2 The sterility test was carried out according to the P448 method of "Regulations of Veterinary Biol...

Embodiment 3

[0081] Embodiment 3 Newcastle disease and H9 subtype avian influenza dual concentrated inactivated vaccine and the immune effect comparative test of chicken Newcastle disease (LaSota strain) and H9 subtype avian influenza (HL strain) dual inactivated vaccine

[0082]1. Whole body and local immune response of small day-old chickens Get 20 SPF chickens of 1-10 days age, divide into two groups, one group is immunized with the prepared vaccine of vaccine preparation formula 1 in Example 1, and each intramuscular injection 0.1ml Another group was immunized with the vaccine purchased from the market (Newcastle disease, avian influenza H9 subtype dual inactivated vaccine; Qilu Animal Health Products Company, batch number 20110230), and each muscle was injected with 0.3ml of the dual vaccine, continuously for 14 days after the injection Observe systemic reactions and local reactions, see Table 4 for details.

[0083] Table 4 Immunization observation results of young day-old chickens ...

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Abstract

The invention provides a combined concentrated and inactivated vaccine of Newcastle disease and H9 subtype avian influenza, which contains an inactivated Newcastle disease virus LaSota strain, an inactivated H9 subtype avian influenza virus HL strain and an adjuvant. The invention further provides a preparation method of the combined concentrated and inactivated vaccine. According to the combinedconcentrated and inactivated vaccine provided by the invention, the immunizing dose of the vaccine is reduced to one third of a traditional vaccine, namely, the immunizing dose of the vaccine is reduced from 0.3 ml to 0.1 ml per chick which is 1-5 weeks old, by means of increasing the antigen content in unit volume of the vaccine so that the stress reaction of vaccine immunity to an organism is reduced. Furthermore, the vaccine provided by the invention is applied to chicken with a small age in days so that the application range of the vaccine is widened. In addition, the immunity effect of the vaccine provided by the invention is not reduced on the premise of reducing the dose of the vaccine. Therefore, the combined vaccine of the Newcastle disease and the avian influenza, provided by the invention, has the advantages of small dose of the vaccine, better immune effect, lower cost and longer immune protection period.

Description

technical field [0001] The invention relates to a dual inactivated vaccine of Newcastle disease and H9 subtype avian influenza and a preparation method thereof, in particular to a dual inactivated vaccine of Newcastle disease and H9 subtype avian influenza with small immune dose and better immune effect and its Preparation. Background technique [0002] Newcastle disease virus (Newcastledisease virus, NDV) is also known as Asian fowl plague virus, pseudo fowl plague virus or avian pneumoencephalitis virus. The position in virus taxonomy belongs to Paramyxoviridae (Paramyxoviridae), a species in Paramyxovirus genus (Paramyxovirus). The virus mainly harms chickens, guinea fowls and turkeys, and spreads rapidly among the infested flocks, and the virulent strain can destroy the whole flock of chickens. Attenuated strains only cause respiratory tract infection and decreased egg production in chickens, but they can recover quickly. Humans can develop conjunctivitis or lymphaden...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/295A61P31/14A61P31/16A61K39/145A61K39/17
Inventor 张许科孙进忠白朝勇
Owner PU LIKE BIO ENG
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