Method for detecting EcSSR0003 microsatellite deoxyribonucleic acid (DNA) markers of palaemon carincauda holthuis

A DNA marker, white shrimp technology, applied in the field of molecular genetic markers, can solve problems such as microsatellite DNA detection of white shrimp that have not yet been seen

Active Publication Date: 2012-01-04
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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But so far, there is no report on microsatel

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  • Method for detecting EcSSR0003 microsatellite deoxyribonucleic acid (DNA) markers of palaemon carincauda holthuis
  • Method for detecting EcSSR0003 microsatellite deoxyribonucleic acid (DNA) markers of palaemon carincauda holthuis

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Embodiment 1

[0019] The detection method of EcSSR0003 microsatellite core sequence DNA molecular marker of white shrimp EcSSR0003 of the present invention comprises the following steps:

[0020] Firstly, the genomic DNA of the white shrimp was extracted and diluted for later use; then, using the microsatellite core sequence containing EcSSR0003 in the white shrimp genome library, specific primers were designed at both ends of the sequence; PCR amplification was performed on the genomic DNA of the individuals within the group, and polyacrylamide gel electrophoresis was used to detect the PCR products; the bands that appeared in the products were used to analyze the genotype of each individual, and the height of the EcSSR0003 core sequence region of the white shrimp was obtained. A polymorphic map of genetic variation (eg figure 1 shown).

[0021] The core sequence of EcSSR0003 of white shrimp is ATAACTTTTCATCTCTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCAATTTTTCTC, and the ...

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Abstract

The invention provides a method for detecting EcSSR0003 microsatellite deoxyribonucleic acid (DNA) markers of palaemon carincauda holthuis. The method comprises the following steps of: extracting DNA of a genome of the palaemon carincauda holthuis, and diluting for later use; designing specific primers at two ends of an EcSSR0003 microsatellite core sequence; performing polymerase chain reaction (PCR) amplification on the DNA of the genome of the palaemon carincauda holthuis by using the primers, and detecting a PCR product; and analyzing by utilizing stripes of the product to obtain a polymorphic map of the palaemon carincauda holthuis subjected to high genetic variation in an EcSSR0003 core sequence area. By the primers and the detection method, the genetic variation of each individual of the palaemon carincauda holthuis in the EcSSR0003 microsatellite core area can be detected, and the polymorphic map of EcSSR0003 genetic marker gene loci, which present the high genetic variation, of the palaemon carincauda holthuis can be obtained quickly. The method is suitable for application of genetic markers, genealogy authentication, the construction of genetic maps and the like of palaemon carincauda holthuis groups.

Description

technical field [0001] The invention belongs to the field of molecular genetic markers, in particular to a method for detecting microsatellite DNA markers of EcSSR0003 white shrimp. Background technique [0002] Microsatellites (microsatellites) or simple sequence repeats (simple sequence repeats, SSR) refers to a simple tandem repeat DNA sequence composed of 1 to 6 nucleotides. It has been found in all biological species studied so far, and its distribution density is very large. Because microsatellites are widely distributed in the genome, they have the characteristics of high density, rich polymorphism, high heterozygosity and good stability, co-dominant inheritance following Mendelian segregation law, and easy PCR amplification. It is an eye-catching new DNA marker, and is widely used in many research fields such as family genealogy authentication of biological resources, gene linkage analysis, genetic map construction, germplasm identification, and population genetic d...

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 刘萍李健贾舒雯
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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