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Separating, purifying and inspecting method of anthocyanin in blueberry wine dregs

A technology of blueberry anthocyanin and blueberry lees, applied in the directions of organic chemistry, color/spectral property measurement, etc., can solve the problems of unreported utilization of blueberry lees, and achieve the prevention of cardiovascular disease, alleviation of liver dysfunction, reduction of The effect of business costs

Inactive Publication Date: 2012-01-18
吉林云尚保健食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the domestic market, blueberry drinks and health wine are relatively popular, but there is no report on the utilization of blueberry lees after drinking

Method used

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  • Separating, purifying and inspecting method of anthocyanin in blueberry wine dregs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Quick-freezing the blueberry wine residue to obtain frozen blueberry wine residue. The blueberry distiller's residue stored at -18°C was taken out, ground and pulverized into a slurry, and blueberry anthocyanins were prepared by extraction, purification, and freeze-drying.

[0032] Extraction process conditions: ①acidified ethanol solution: ethanol concentration 90%, pH value 2.0; ②extraction material liquid: blueberry wine residue by weight:acidified ethanol=8:120; ③extraction temperature: 60°C, extraction times 4 times, the total extraction time is 8 hours; ⑤ Concentration under reduced pressure after extraction: the vacuum degree can be 0.075-0.095Mpa, the temperature is 60°C and the concentration is 1 / 5-1 / 20 of the original volume, and there is no alcohol smell.

[0033] Purification process conditions: ①Single-column chromatography, AB-8 macroporous resin with diameter-to-height ratio of 1:30; ②Adsorption conditions: column chromatography sample volume is 15ml / min,...

Embodiment 2

[0036] After the blueberry distiller's residue was ground into a slurry, blueberry anthocyanins were prepared by a combination of extraction and freeze-drying.

[0037]Extraction process conditions: ①acidified ethanol solution: ethanol concentration 80%, pH value 3.0; ②extraction material liquid: blueberry lees:acidified ethanol=8:50 by weight; ③extraction temperature: 50°C, extraction times 2 times, the total extraction time is 2 hours; ⑤ Concentration under reduced pressure after extraction: the vacuum degree can be 0.075-0.095Mpa, the temperature is 45°C and the concentration is 1 / 5-1 / 20 of the original volume, and there is no alcohol smell.

[0038] Purification process conditions: ①Single-column chromatography, diameter-to-height ratio of polyamide resin 1:10; ②Adsorption conditions: column chromatography sample volume is 10ml / min; operating temperature 30°C, adsorption pH 6.0; ③Eluent Conditions: the concentration of ethanol in the eluent is 40%; the elution flow rate is...

Embodiment 3

[0041] The dried blueberry distiller's residue is obtained by freeze-drying the blueberry distiller's residue, which is ground and pulverized into a 40-100 mesh powder, and then the blueberry anthocyanin is prepared by extracting, purifying and freeze-drying.

[0042] Extraction process conditions: ①acidified ethanol solution: ethanol concentration 70%, pH value 3.0; ②extraction material liquid: blueberry lees by weight:acidified ethanol=1:25; ③extraction temperature: 40°C, extraction times 2 times, the total extraction time is 12h; ⑤ Concentration under reduced pressure after extraction: the vacuum degree can be 0.075-0.095Mpa, the temperature is 60°C and the concentration is 1 / 5-1 / 20 of the original volume, and there is no alcohol smell.

[0043] Purification process conditions: ①Single-column chromatography, diameter-to-height ratio of polyamide resin 1:30; ②Adsorption conditions: column chromatography sample volume is 5ml / min; operating temperature 30 ° C, adsorption pH 5.0...

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Abstract

The invention relates to a separating, purifying and inspecting method of anthocyanin in blueberry wine dregs, which belongs to the technical field of functional active constituent extraction and purification. In the separating method, blueberry wine dregs or frozen blueberry wine dregs are ground into slurry or are freeze-dried and crushed into powder; and the slurry or the powder is separated in a warm extraction method in an acidified ethanol solution, and blueberry anthocyanin coarse extracting liquid is obtained through reduced pressure concentration. The blueberry anthocyanin coarse extracting liquid is purified and concentrated to prepare a blueberry anthocyanin concentrated solution. The concentrated solution is freeze-dried to obtain blueberry wine dreg anthocyanin freeze-dried powder. In the invention, the anthocyanin is separated out from waste produced by blueberry wine, which shows the research and development of simple, efficient, environment-friendly and low-cost production technology; and the content of the anthocyanin in the blueberry anthocyanin freeze-dried powder is 10-40%. The anthocyanin concentrated solution or coarse extracting liquid can be directly manufactured into oral liquid, tablets and other functional foods. The anthocyanin freeze-dried powder can be manufactured into food stain with an obvious antioxidation to be widely used in the fields of beverages, wines and the like.

Description

technical field [0001] The invention belongs to the technical field of extraction and purification of functional active ingredients and development of functional foods, and relates to a method for separating and purifying anthocyanins from blueberry wine lees. Background technique [0002] Blueberry, also known as lingonberry, Dusi, ground fruit, the fruit is divided into red, blue, and white three colors, and European and American countries call the blue fruit blueberry. Blueberry is a wild plant with strong cold resistance, which can withstand the severe cold of -50°C. It is native to North America, Scotland, and Russia. I used to be mainly distributed in Northeast China, Inner Mongolia, Xinjiang and other regions. Blueberries are very rich in nutrients, and the content of anthocyanins and SOD is many times higher than that of other plants. The nutritional and health functions of blueberries have attracted the attention of many scholars at home and abroad. The research di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62G01N21/33
Inventor 杜丽华徐道田李长久
Owner 吉林云尚保健食品有限公司
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