The invention discloses a beverage capable of nourishing yin, clearing heat, and quenching thirst. The beverage is prepared from, by weight, 40 to 50 parts of fresh dendrobium nobile, 25 to 35 parts of grape, 3 to 8 parts of raw rhizoma rehmanniae, 3 to 6 parts of rhizoma discoreae, 2 to 5 parts of radix trichosanthis, 2 to 5 parts of rhizoma alismatis, 2 to 5 parts of radix ophiopogonis, 3 to 5 parts of rhizoma anemarrhenae, 5 to 10 parts of astragalus membranaceus, 2 to 10 parts of radix puerariae, 2 to 10 parts of polygonum multiflorum, 1 to 2 parts of loofah sponge, an appropriate amount of honey, and an appropriate amount of water. The beverage is capable of nourishing yin, clearing heat, quenching thirst, and adjusting human physiological functions. The mouthfeel of a product prepared via a preparation method disclosed in the invention is sour, sweet, and fresh; the product is rich in dendrobium polysaccharides, is high in phamaceutical value, and possesses excellent health care functions; long term drinking of the beverage is capable of nourishing yin and beautifying the features, changing skin color, resisting aging, and prolonging lifetime. The beverage is convenient to carry and drink, is capable of promoting stomach and intestine peristalsis, treating diseases, and improving human body immunity.