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Preparation method, product and application of lactobacillus whole peptidoglycan with effect for resisting beta-lactoglobulin hypersusceptibility

The technology of lactoglobulin and peptidoglycan is applied in microemulsion preparation, preparation of complete peptidoglycan, preparation of peptidoglycan, and application field of immune enhancer, which can solve the problem of low oral bioavailability and adverse reactions, etc. problem, to achieve the effect of improving bioavailability, improving solubility, and simple preparation method

Inactive Publication Date: 2014-07-09
天津众品科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing cell wall peptidoglycan is basically administered by injection, which has the problems of low oral bioavailability and adverse reactions caused by excessive doses

Method used

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  • Preparation method, product and application of lactobacillus whole peptidoglycan with effect for resisting beta-lactoglobulin hypersusceptibility
  • Preparation method, product and application of lactobacillus whole peptidoglycan with effect for resisting beta-lactoglobulin hypersusceptibility
  • Preparation method, product and application of lactobacillus whole peptidoglycan with effect for resisting beta-lactoglobulin hypersusceptibility

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The preparation of embodiment 1 lactobacillus complete peptidoglycan, complete peptidoglycan microemulsion

[0029] 1.1.1 Experimental strains

[0030] Lactobacillus johnsonii KLDS1.7032 was screened from the intestinal tract of infants. Lactobacillus johnsonii KLDS1.7032 was preserved in the General Microbiology Center of China Committee for the Collection of Microorganisms. Its strain preservation number is CGMCC 5050 and the preservation date is July 2011. August 8.

[0031] 1.1.2 Experimental reagents

[0032] Hydrochloric acid, concentrated sulfuric acid, phosphoric acid, sodium chloride, sodium hydroxide, disodium hydrogen phosphate, sodium dihydrogen phosphate, sodium carbonate, absolute ethanol (food grade), ethyl n-butyrate, formaldehyde, methanol, acetone, peroxide Hydrogen, perchloric acid, ascorbic acid, acetic acid, diethyl ether, lactone acetate, phenol, chloroform, glucose, cholesterol, lysozyme, Tris, sodium acetate, phenol, Triton X-100, SDS, trypsin,...

Embodiment 2

[0080]Example 2 Intervention effect of oral whole peptidoglycan microemulsion on allergic reaction of sensitized model animals

[0081] The complete peptidoglycan microemulsion and peptidoglycan prepared in Example 1 were used for the following experimental research:

[0082] 2.1 Experimental materials:

[0083] 2.1.1 Experimental animals

[0084] Female clean-grade BALB / c mice, 6-8 weeks old, were purchased from the Experimental Animal Center of Harbin Cancer Hospital. The temperature of the feeding environment is (23±2)°C, the humidity is 50% to 75%, and the mice are fed with standard mouse feed.

[0085] 2.1.2 Main reagents

[0086] The peptidoglycan extract prepared in Example 1, the peptidoglycan microemulsion prepared in Example 1, IL-12, IFN-γ, IL-4 ELISA kit (U.S. R&D Company), total IgE kit (U.S. bethyl company), goat anti-mouse IgE enzyme-labeled secondary antibody (AbD Serotec, UK), goat anti-mouse IgG enzyme-labeled secondary antibody (China Zhongshan Jinqiao C...

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Abstract

The invention discloses a preparation method, a product and application of lactobacillus whole peptidoglycan with an effect for resisting beta-lactoglobulin hypersusceptibility and relates to a microemulsion preparation prepared from the lactobacillus whole peptidoglycan obtained in the invention. The microemulsion preparation is prepared by the following steps of: with the lactobacillus whole peptidoglycan as a main effective component, heating the whole peptidoglycan, an oil phase, a surfactant, a cosurfactant and water at 70DEG C by stirring to enable the whole peptidoglycan to be completely dissolved; and uniformly mixing to obtain a semitransparent emulsion product with the liquid drop diameter of 30-45nm. After the lactobacillus whole peptidoglycan microemulsion preparation is orally taken to enter the interior of the body, lymphocyte can be effectively stimulated to secrete IL-12 and IFN-gamma; the IL-4 level is reduced; excessive hyperfunction of Th2 cells is reversed; and the lactobacillus whole peptidoglycan microemulsion preparation plays an important role of inhibiting beta-lactoglobulin allergic inflammation mediated by IgE and has potential application values for developing an immunopotentiator with antiallergic performance and the like.

Description

technical field [0001] The present invention relates to a preparation method and application of peptidoglycan, in particular to a method for preparing a complete peptidoglycan of Lactobacillus, and to a microemulsion prepared from the complete peptidoglycan, its preparation method and its application in Application in preparation of immune enhancer for anti-beta-lactoglobulin allergy. It belongs to the field of biopharmaceuticals. Background technique [0002] Milk is rich in nutrients and is an important food protein source for infants and young children. However, according to foreign epidemiological surveys, 2% to 6% of children have allergic reactions to cow's milk, which seriously affects their absorption and utilization of high-quality milk protein. β-lactoglobulin (β-lactglobulin) is regarded as one of the most important bovine milk allergen proteins, β-lactoglobulin has a function similar to immunoglobulin for calves, but it is the main allergen for infants , easy ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/16C08B37/00C12P1/04A61P37/04C12R1/225
Inventor 李艾黎孟祥晨杜鹏马冬雪
Owner 天津众品科技有限公司
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