Cell culture device

Abstract

The invention relates to a cell culture device, which comprises fixing base plates and a cell culture system, a light control system and an electrode system which are fixed on the fixing base plates. The cell culture device adopts a special structure of a microfluidic technology to realize culturing of nerve cells, for example, the nerve cells can grow in a cell culture chamber, while axons can only grow in nerve conduits, the nerve cells in the cell culture chamber through the axons in the nerve conduits to constitute nerve loops, and the reproductivity and the controlling force of a cell growth area are relatively strong; the adjustment and the control with high spatiotemporal resolution are performed on the nerve cells through laser, the selectivity and the specificity are strong, and the excitement and the inhibition of the nerve cells can be realized; the nerve loops are excited through the electrode system to carry out auxiliary adjustment and control, the behavior and the function of the nerve loops are monitored in a real-time manner, the spatiotemporal resolution is high, and the adjustment and control as well as detection results are reliable.

Description

【Technical field】 [0001] The invention relates to the field of neural engineering, in particular to a cell culture device based on microfluidic technology. 【Background technique】 [0002] With the increasing incidence of nervous system diseases, its research is gradually becoming a hot spot. However, due to the complexity of the nervous system, it is very difficult to study some problems of the nervous system in vivo, especially the exploration of neural circuits and neural plasticity. Under such conditions, in vitro culture of nerve cells becomes an ideal method. Regular connections between neurons can be established through in vitro culture, and the complex three-dimensional connections between neurons can be reduced to two dimensions, making observation and research easier. [0003] The traditional method of culturing nerve cells in vitro is to regulate the growth of nerve cells on the culture medium through chemically patterned surface, physical microstructure construc...

AI Technical Summary

Problems solved by technology

[0005] However, by regulating the growth of nerve cells to form neural circuits through surface chemical treatment, physical microstructure construction or chemical concentration gradients, the reproducibility and control of the cell growth area is relatively poor, so the reproducibility of the experiment is poor.

Although functional electrical stimulation has excellent time resolution, its spatial resolution is poor, and it is not selective and specific, making it difficult to inhibit nerve cells

Although local perfusion has high spatial resolution, the position of perfusion is random, it is difficult to control accurately, and the reproducibility is poor; in addition, the time resolution of local perfusion is poor, which cannot meet the electrophysiological requirements

Therefore, the traditional lack of a cell culture device that can be used for nerve cell culture, neural circuit construction, and regulation and detection at a higher spatiotemporal resolution (Spatiotemporal resolution)

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