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Method for microwave extraction of anthocyanin, resveratrol and piceid from grape cell

A technology of resveratrol glycosides and grape cells, which is applied in the field of plant active ingredient extraction, can solve the problems of no extraction of resveratrol and resveratrol glycosides, complicated process operations, low product quality, etc., and achieves extraction The effect of shortening time, simple process and high extraction efficiency

Inactive Publication Date: 2012-02-15
TIANJIN ACELBIO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In general, this type of method has complicated process operation, long cycle and low product quality
[0006] At present, the traditional heating and reflux extraction method is mostly used at home and abroad, and there is no report on the use of microwave method to extract resveratrol and resveratrol glycosides in grape cells

Method used

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  • Method for microwave extraction of anthocyanin, resveratrol and piceid from grape cell
  • Method for microwave extraction of anthocyanin, resveratrol and piceid from grape cell
  • Method for microwave extraction of anthocyanin, resveratrol and piceid from grape cell

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Comparison scheme
Effect test

Embodiment 1

[0027] A method for microwave extraction of anthocyanins, resveratrol and resveratrol glycosides in grape cells, comprising the steps of:

[0028] The fresh grape cells obtained by the solid culture method are suction-filtered, and the grape cells are mixed with the volume concentration of 30% ethanol aqueous solution according to the ratio of the mass ratio of material to liquid of 1:4, and the microwave extraction equipment is used at 80°C and the microwave power is 500W Under the conditions, extract for 5 minutes, filter, repeat the extraction 4 times, combine the 4 extracts at 60°C, vacuum degree ≤ -65cmHg, concentrate under reduced pressure to form an extract, dry to obtain anthocyanin, resveratrol and white Veratrol glycosides powder.

Embodiment 2

[0030] A method for microwave extraction of anthocyanins, resveratrol and resveratrol glycosides in grape cells, comprising the steps of:

[0031] The fresh grape cells obtained by the solid culture method were suction-filtered, and the grape cells were mixed with water according to the ratio of solid-liquid mass ratio of 1:7, and extracted by microwave extraction equipment at 90°C and microwave power of 1000W for 10 minutes. Filtrate, repeat the extraction twice, combine the two extracts, concentrate under reduced pressure at 60°C and vacuum ≤ -65cmHg to form an extract, and dry to obtain anthocyanin, resveratrol and resveratrol glycosides powder.

Embodiment 3

[0033] A method for microwave extraction of anthocyanins, resveratrol and resveratrol glycosides in grape cells, comprising the steps of:

[0034] The fresh grape cells obtained by the suspension culture method are suction-filtered, and the grape cells are mixed with the ethanol aqueous solution with a volume concentration of 40% according to the mass ratio of material to liquid of 1:10. Under the condition of 200W, extract for 1min, filter, repeat the extraction 3 times, combine the 3 extracts, and concentrate under reduced pressure at 60°C and vacuum degree ≤ -65cmHg to obtain an extract, and dry to obtain anthocyanin, resveratrol Powder of alcohol and resveratrol glycosides.

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Abstract

The invention discloses a method for microwave extraction of anthocyanin, resveratrol and piceid from a grape cell. The method comprises the steps of: adding an ethanol water solution into a grape cell obtained by plant cell culture, conducting stirring extraction with a microwave extraction device at a temperature of 30-90DEG C and at power of 100-1000W, subjecting the obtained extracting solution to reduced pressure condensation so as to obtain extract extractum, drying the extractum, thus obtaining powder containing anthocyanin, resveratrol and piceid. The method of the invention overcomesthe defects of long solvent extraction time, large solvent consumption, great solvent residual amount, high energy consumption, high cost, complex operation process, difficult enlargement, poor production practicality, relatively low recovery rate and poor product quality etc. in traditional methods. The method provided in the invention has the advantages of uniform heating, shortened extraction time, energy conservation, less solvent consumption, high extraction efficiency, good selectivity, simple technology, less pollution, simple equipment, as well as less investment.

Description

technical field [0001] The invention relates to the field of extraction of effective plant components, in particular to a microwave extraction method for anthocyanins, resveratrol and resveratrol glycosides in grape cells. Background technique [0002] Decades ago, scientists discovered a kind of phytoalexin in grape plants to prevent grapes from rotting, that is, resveratrol (Res for short) and its glucoside resveratrol glycoside (Piceid) . Modern pharmacological studies have shown that these compounds have biopharmacological activities such as anti-tumor, anti-cardiovascular disease, treatment of mutations, anti-oxidation, anti-bacterial and anti-inflammatory, liver protection, induction of cell apoptosis and regulation of estrogen, and are widely used in medicine, food , cosmetics and other industries. Resveratrol is now found in at least 72 species of plants belonging to 21 families and 31 genera (Jang M, Cai L, Udeani GO, et al. Cancer chemo-preventive activity of res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62C07C39/21C07C37/70C07H15/203C07H1/06
Inventor 张卫胡雪艳吴秀祯吕昕泽龚卫东石万利
Owner TIANJIN ACELBIO BIOTECH