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Detection method for dextroisomer in levocarnitine

A detection method and isomer technology, applied in the field of drug analysis, can solve problems such as no related reports

Active Publication Date: 2012-02-22
NORTHEAST PHARMA GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no relevant report on the detection method of levocarnitine dextro isomer

Method used

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  • Detection method for dextroisomer in levocarnitine
  • Detection method for dextroisomer in levocarnitine
  • Detection method for dextroisomer in levocarnitine

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Embodiment Construction

[0024] The following examples will help to understand the present invention, but the examples are only to illustrate the present invention, and the present invention is not limited to these contents.

[0025] 1. Chromatographic conditions:

[0026] Chromatographic column: C18 column filled with octadecylsilane bonded silica gel, particle size 5μm; detection wavelength: excitation wavelength λex=260nm, fluorescence wavelength λem=315nm; mobile phase: tetra-n-butylammonium hydroxide-methanol and acetonitrile - Water; column temperature: 30°C, injection volume 5 μl.

[0027] 2. Preparation of relevant solutions and mobile phases:

[0028] 2.1 Preparation of relevant solutions

[0029] 2.1.1 Preparation of tetra-n-butylammonium hydroxide solvent:

[0030] Accurately draw 24.4ml of 55% tetra-n-butylammonium hydroxide reagent, dilute to 1000ml with water, shake well, and adjust the pH to 7.0 with phosphoric acid.

[0031] 2.1.2 Preparation of D-Fremoxycarbonyl Chloride Reagent ...

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Abstract

The invention provides a detection method for dextroisomer in levocarnitine, which belongs to the field of pharmaceutical analysis. The detection method comprises the following steps: preparing chromatographic conditions, that is, using a C18 column with a filling agent of octadecylsilane chemically bonded silica and a particle size of 5 mu m as a chromatographic column, setting detection wavelength of excitation wavelength lambda ex and fluorescence wavelength lambda em to be 260 nm and 315 nm respectively, and using tetra-n-butylammonium hydroxide-methanol and acetonitrile-water as mobile phases; preparing a solvent of tetra-n-butylammonium hydroxide; preparing a reagent of fluorenylmethyl chloroformate; preparing a system suitability solution; preparing a solution of a test sample; carrying out a derivative reaction; carrying out detection by using HPLC (using a fluorescence detector and the method of gradient elution). The method provided in the invention has reproducibility, sensitivity and accuracy according with requirements when used for detection of dextroisomer in levocarnitine.

Description

technical field [0001] The invention relates to a method for detecting the dextro isomer in levocarnitine in the field of drug analysis. Background technique [0002] L-carnitine, also known as L-carnitine, was first discovered in muscle extracts by two Russian scientists in 1905, and its molecular formula is 3-hydroxy-4-aza-trimethylaminobutyric acid. Levocarnitine is a special amino acid that widely exists in body tissues. It is an essential cofactor for fatty acid metabolism. It can promote fatty acids to enter the tricarboxylic acid cycle through B-oxidation and generate energy. It is closely related to the metabolism of organs and tissues in the body. . The lack of L-carnitine will lead to energy supply disorder and acidosis of various intermediate products of fatty acid metabolism, and symptoms such as cardiomyopathy, arrhythmia, and body fatigue will occur. There are two optical isomers of L-carnitine, the L-isomer and the D-isomer, but only the L-isomer has biologi...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 孟涛冯文宇娄玉涛尤楠金玉坤尹宏苏贵弟孟喆周岩唐奎山刁恩德刘爱泉
Owner NORTHEAST PHARMA GRP
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