Modified bacillus source and Alpha-amylase gene recombination lactobacillus as well as product and application thereof

Abstract

The invention discloses a modified bacillus source and Alpha-amylase gene recombination lactobacillus as well as a product and the application thereof. An Alpha-amylase gene and a signal peptide gene are connected, are then inserted into a multiple cloning site of a lactobacillus expression vector pIlac, and are further integrated into a ribosome bind site of lactobacillus casei L.CECT5276, wherein the Alpha-amylase gene is a starch liquefaction bacillus with an accession number of GU591658, and a sequence used for amplification is a 1-1545bp basic group; and the signal peptide gene is a short lactobacillus L.brevis1.2028 with an accession number of Z14250, and a sequence used for amplification is a 260-349bp basic group. The recombination lactobacillus can be applied to the production of animals, of which the feed is rich in starch, particularly newborn piglets and chicken, and has great significance in improving the growth speed, reducing the feed conversion ratio, increasing the economic benefits and the like.

Description

technical field [0001] The invention relates to a gene recombination technology, in particular to a recombinant lactobacillus with a bacillus-derived α-amylase gene, its products and its application. Background technique [0002] As we all know, the growth of animals depends on nutrient-rich feed, and the speed of animal growth depends on the digestion and utilization of feed by animals, and the digestion and utilization of feed depends on the amount of digestive enzymes in its digestive tract, because Most of the nutrients in the feed exist in the form of polymers, so there must be corresponding enzymes to degrade them before they can be absorbed and utilized by the intestines. Therefore, the amount of digestive enzymes in the intestines is very important for animal growth, feed-to-meat ratio and economic benefits. very important. At present, many digestive enzymes such as amylase, cellulase, protease, etc. have been developed and widely used in feed. In feed ingredients,...

AI Technical Summary

Problems solved by technology

[0005] However, naturally isolated strains often have low α-amylase activity and are difficult to be directly used in industrial production or as additives. Therefore, wild-type strains are generally modified, such as through mutation breeding, construction of genetically engineered bacteria and genes Mutation, so that α-amylase can be expressed in specific host bacteria, and the enzyme production is also greatly improved

Images