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High-fluorescent brightness quantum dot composite particle, immunological detection probe and preparation method of the high-fluorescent brightness quantum dot composite particle

A technology of composite particles and fluorescent brightness, applied in the field of immunological detection probes, can solve the problems of quantum dot chemistry, physical instability, wide emission spectrum, easy photobleaching, etc.

Inactive Publication Date: 2012-03-21
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The key technology for quantum dots to be used in immunological detection is to prepare effective detection probes. In the process of immunological detection, the probes are required to have high luminescence brightness, high stability, and high resistance to photobleaching. The purpose is to increase the detection sensitivity and Accuracy; Although traditional organic light-emitting molecules can also be used as fluorescent probes, they have disadvantages such as low signal intensity, easy photobleaching, narrow excitation spectrum and wide emission spectrum, which seriously restrict their use in actual production and life. Applications
Compared with organic light-emitting molecules, semiconductor quantum dots show many advantages and become the first choice for the preparation of effective detection probes; however, due to the chemical and physical instability of quantum dots themselves, their fluorescence sensing performance is very susceptible to be affected. The influence of the test environment, which greatly affects the accuracy and repeatability of the detection response of the substance to be tested

Method used

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  • High-fluorescent brightness quantum dot composite particle, immunological detection probe and preparation method of the high-fluorescent brightness quantum dot composite particle
  • High-fluorescent brightness quantum dot composite particle, immunological detection probe and preparation method of the high-fluorescent brightness quantum dot composite particle
  • High-fluorescent brightness quantum dot composite particle, immunological detection probe and preparation method of the high-fluorescent brightness quantum dot composite particle

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Experimental program
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Effect test

Embodiment 1

[0034] 1.1 The CdTe quantum dots were synthesized by the aqueous phase method. First synthesize the NaHTe required for the preparation of CdTe quantum dots: combine Te powder with NaBH 4 Mix according to the molar ratio of 1:5, add a certain amount of water, heat and stir in a 60°C water bath under the protection of nitrogen until the solution turns lavender. 0.4 mmol of CdCl 2 2.5H 2 O was dissolved in 25 mL of water, 0.6 mmol of thioglycolic acid (TGA) was added, stirred, and then 1M NaOH solution was added until the pH value of the solution was adjusted to 11.2. The solution was deoxygenated with nitrogen for 30 minutes, then injected into the NaHTe solution prepared above, and heated to reflux at 100 °C for 0.5 h to obtain water-soluble CdTe quantum dots (particle size: 2.6 nm) emitting green light. The quantum dots are precipitated from the solution by ethanol and isopropanol, centrifuged, and the obtained quantum dots are dispersed in water.

[0035] 1.2 Take 1 mL of...

Embodiment 2

[0040] 2.1 The CdTe quantum dots were synthesized by the aqueous phase method. First synthesize the NaHTe required for the preparation of CdTe quantum dots: combine Te powder with NaBH 4 Mix according to the molar ratio of 1:5, add a certain amount of water, heat and stir in a 60°C water bath under the protection of nitrogen until the solution turns lavender. 0.4 mmol of CdCl 2 2.5H 2 O was dissolved in 25 mL of water, 0.6 mmol of thioglycolic acid (TGA) was added, stirred, and then 1M NaOH solution was added until the pH value of the solution was adjusted to 11.2. The solution was deoxygenated with nitrogen for 30 minutes, then injected into the NaHTe solution prepared above, and heated to reflux at 100 °C for 24 h to obtain water-soluble red-emitting CdTe quantum dots (particle size 4 nm). The quantum dots are precipitated from the solution by ethanol and isopropanol, centrifuged, and the obtained quantum dots are dispersed in water.

[0041] 2.2 Take 1mL of quantum dots...

Embodiment 3

[0046] 3.1 The CdTe quantum dots were synthesized by the aqueous phase method. The preparation method is as described in 2.1 of Example 2 above.

[0047] 3.2 Take 1.5mL of quantum dots, the concentration of quantum dots is 1.2 × 10 -6 M, add 0.5 μL of mercaptopropyltrimethoxysilane, and stir for 5 hours.

[0048] 3.3 Mix 2 mL of the alkoxysilanized quantum dot colloid solution described in preparation step 3.2 with 8 mL of propanol, add 0.8 mL of 5 wt % ammonia water, and stir for 15 hours to complete the self-assembly of quantum dots.

[0049]3.4 Add 16 μL of propyl orthosilicate and 0.2 mL of 10wt% ammonia water to the solution of the self-assembled quantum dots described in the preparation step 3.3, then stir for 3 hours, add 1 μL of mercaptopropyltrimethoxysilane and continue stirring for 10 hours. SiO with evenly distributed quantum dots in the center and mercapto groups on the surface was obtained after centrifugation 2 Particles, electron microscope photos as figu...

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Abstract

The invention discloses a high-fluorescent brightness quantum dot composite particle. The high-fluorescent brightness quantum dot composite particle is characterized in that at least one quantum dot is coated with a silica shell on the surface to form a composite particle, wherein the composite particle has a diameter of 5 to 100 nanometers and a mole ratio of SiO2 to the at least one quantum dot is in a range of (60 to 99): (0.2 to 40). The invention also discloses a preparation method of the high-fluorescent brightness quantum dot composite particle and an immunological detection probe prepared form the high-fluorescent brightness quantum dot composite particle. The high-fluorescent brightness quantum dot composite particle obtained by the preparation method has good biological adaptability and environmental stability. The preparation method of the high-fluorescent brightness quantum dot composite particle realizes good combination of the high-fluorescent brightness quantum dot composite particle and a biomolecule. The immunological detection probe prepared form the high-fluorescent brightness quantum dot composite particle has high application values in the fields of medicine and biotechnology.

Description

technical field [0001] The invention relates to a quantum dot composite particle with high fluorescence brightness, an immunological detection probe made from the composite particle, and a preparation method of the composite particle. Background technique [0002] Since the research on the use of quantum dots for fluorescent probes was reported by the American "Science" magazine in 1998, scientists from various countries have begun to pay attention to the use of luminescent quantum dots in biological detection systems, especially the research on various fluorescent probes for tracking; It is forward-looking that luminescent quantum dots are used for immunological detection. For example, some people use quantum dot probes for clinical detection of breast cancer. Its convenient and fast method creates a good platform for immunological detection. The key technology for quantum dots to be used in immunological detection is to prepare effective detection probes. In the process of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02C09K11/88C09K11/56C09K11/70C12N15/11C12Q1/68G01N33/53
Inventor 杨萍于京华
Owner UNIV OF JINAN
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