Small spore phoma microspora for controlling conyza sumatrensis

A technology for Sumatran liquor and liquor grass, which is applied in the field of microsporum sclerotiorum, can solve the problems of labor and time-consuming artificial weeding, affecting economic structure and social stability, environmental pollution, etc. Application value, effect of strong disease control effect

Inactive Publication Date: 2012-03-21
SOUTHWEST UNIVERSITY
View PDF1 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Sumen baijiu grass is native to South America and was introduced to China in the middle of the 19th century. In recent years, Sumen baijiu grass grows rapidly, reproduces fast, produces a large number of seeds, and is widely distributed (especially along the Yangtze River). Around them, almost The growth of native plants cannot be seen, especially in places where the original vegetation has been destroyed. This kind of plants take advantage of the gap and preempt growth, forming a growth advantage, which not only causes a large reduction in the output of field crops, but also affects the economic structure and society. Stable, and because it grows in large quantities in the Yangtze River Basin, it is easy to cause ecological security and cause serious consequences. It is a typical ba

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Small spore phoma microspora for controlling conyza sumatrensis
  • Small spore phoma microspora for controlling conyza sumatrensis
  • Small spore phoma microspora for controlling conyza sumatrensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1. Isolation, purification and screening of Pheopointomyces microsporum

[0022] 1.1 Potato sucrose agar medium (PDA) for fungal isolation and culture, its formula is 200g of peeled fresh potatoes, 20g of glucose and agar each, add tap water to 1000mL. First add water to boil the potatoes, filter through double-layer gauze, add sucrose and agar, add an appropriate amount of water to 1000mL, stir well and put them into a triangular flask, put them in a sterilizer to sterilize and place them. Heat and melt before use, pour into sterilized petri dishes or test tubes to make flat or slant medium for fungal isolation and culture or strain preservation.

[0023] 1.2 Separation, cultivation and purification: Collect samples of S. chinensis plants with typical disease symptoms under natural conditions, rinse and cut off small pieces (2 mm) of the tissue at the junction of leaf disease spots and healthy parts, and put them in 5% sodium hypochlorite After surface disi...

Embodiment 2

[0027] Embodiment 2. Cultivation and inoculation condition test of Pheopointomyces microsporum

[0028] 2.1 Temperature test: On the potato dextrose agar (PDA) medium plate cultivated for 5 days, the mycelia pieces of the SMBC22 strain with a diameter of 0.5 cm were taken with a puncher, and inoculated on the PDA medium, respectively set at 10°C, 15°C, 8 temperature treatments of 20°C, 25°C, 30°C, 35°C and 40°C, each treatment was repeated 3 times. Place them in 7 pre-set light incubators with the required temperature for cultivation, and use the vertical cross method to measure the colony size every day, once every 24 hours, measure 5 times continuously, and record the data. The pH of the PDA medium is about pH7.0. Therefore, the optimum growth temperature of Phomyosporum microsporum was determined.

[0029] 2.2 pH experiment: PDA medium was used in the experiment, and 11 pH treatments were set up. The pH value of the medium was adjusted to 4.0, 5.0, 6.0, 6.5, ...

Embodiment 3

[0032] Embodiment 3. pathogenic bacteria crude toxin separation

[0033] 3.1 Preparation of pathogen culture filtrate: Pour 250 mL of potato dextrose (PD) culture solution into 500 mL Erlenmeyer flasks, sterilize and set aside. Select a colony with good growth for 6-7 days, use a puncher to take a Φ6 mm-sized bacterial cake, inoculate it into a triangular flask containing 250 mL of PS culture solution, inoculate 8 pieces in each bottle, and inoculate it in a 25 °C incubator for 12 h of light / 15 days of static culture under the condition of day. On the ultra-clean workbench, filter with qualitative filter paper (medium speed) to obtain the culture filtrate. Thus prepare enough pathogenic culture filtrate for toxin separation and extraction.

[0034] 3.2 Toxin extraction and activity test: organic solvent extraction: extract the culture filtrate with ethyl acetate, and extract each volume of culture filtrate with an equal volume of ethyl acetate for 3 times, each ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses small spore phoma microspora SMBC022 with a biological activity for controlling conyza sumatrensis, which belongs to the field of biological control over agricultural weeds. The small spore phoma microspora is separated from a host in a natural environment and is identified to be small spore phoma microspora according to morphological characteristics and molecular biology;the optimal culture medium for growing the small spore phoma microspora is a PDA (potato dextrose agar); the optimal temperature is 28 DEG C; and the optimal pH is 7.2. Pathogenic crude toxins can beobtained from a fungal culture liquid through ethyl acetate extraction. The suspension liquid of the microspora and toxin solution thereof do not have adverse effect on 13 important crops such as rice; the SMBC022 strain preparation and the toxin solution have very strong pathogenic and weed-killing effects on malignant weed conyza sumatrensis, and can be produced into an environment-friendly fungal weedicide by a fermented bacterium or toxin biomimetic synthesizing technology. The small spore phoma microspora SMBC022 has important commercial developmental value and application value.

Description

technical field [0001] The invention relates to the technical field of biological control of agricultural weeds, in particular to the use of pathogenic microorganisms with strong pathogenic and control effects on agricultural weeds as an environment-friendly biological herbicide, specifically a plant used to prevent and control Sumen liquor Phomosporum microsporum of grasses. Background technique [0002] Sumen baijiu grass is native to South America and was introduced to China in the middle of the 19th century. In recent years, Sumen baijiu grass grows rapidly, reproduces fast, produces a large number of seeds, and is widely distributed (especially along the Yangtze River). Around them, almost The growth of native plants cannot be seen, especially in places where the original vegetation has been destroyed. This kind of plants take advantage of the gap and preempt growth, forming a growth advantage, which not only causes a large reduction in the output of field crops, bu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/14A01P13/02C12R1/645
Inventor 刘佳谭万忠
Owner SOUTHWEST UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products