Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Mutagenic screening method of DHA-producing strain crypthecodinium. cohnii

A dinoflagellate, mutagenic screening technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of low DHA oil production, achieve excellent industrial production prospects, and reduce screening links and work. The effect of improving the amount and biosynthesis

Inactive Publication Date: 2012-03-28
XIAMEN KINGDOMWAY GROUP
View PDF0 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among the currently published patents on the production of DHA using marine microalgae, the yield of DHA oil is not high, and the reason is related to the strains used and the fermentation culture process adopted.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0025] A kind of mutagenesis screening method of DHA producing bacterium dinoflagellates of the present invention, its steps are as follows:

[0026] 1. Seed cultivation and seed solution pretreatment

[0027] Dinoflagellates ( Crypthecodinium. cohnii ATCC30556) was inoculated in the seed medium, the inoculum volume percentage was 10%, the culture temperature was 26°C, the shaker speed was 200r / min, and cultured for 36h to the logarithmic phase.

[0028]Take 2.5 mL of the above-mentioned cells cultured to the logarithmic phase, centrifuge at 4000 r / min for 10 min, wash 3 times with 0.2 mol / L phosphate buffer, and dilute 50 times, break up with sterile glass beads, 6 layers without Single-cell suspension was obtained by filtering with bacterial gauze.

[0029] 2. Preparation of protoplasts and ultraviolet mutagenesis treatment

[0030] The protoplastization reaction solution was 5 mL, including 0.2 mL of single cell suspension, 0.2 mL of helicase enzyme solution and 4.6...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a mutagenic screening method of a DHA-producing strain crypthecodinium. cohnii. According to the invention, a protoplast of a DHA-producing strain crypthecodinium. cohnii ATCC30556 is prepared; single or composite mutagenesis of ultraviolet + LiCl and diethyl sulfate is carried out upon the protoplast; a basic thought of metabolism regulation and fermentation cultivation is adopted, and oriented screening is carried out by using malonic acid and / or a high-glucose resistance plate; and preliminary screening is carried out by using a solid cultivation medium of red tetrazoline (TTC), such that screening efficiency can be effectively improved, screening links can be reduced, and work load can be reduced. The strain obtained from the preliminary screening step is processed through shake-flask secondary screening and hereditary stability verification. As a result, the growth activity of the strain after mutagenesis is improved. Compared to an initial strain, the DHA biosynthesis amount of an YVD-4 mutagenic strain is improved by 4.7 times, and reaches 45.3g / L. Therefore, the method has an excellent prospect of industrialized production.

Description

technical field [0001] The invention relates to a method for mutagenesis screening of DHA-producing bacteria dinoflagellates. Background technique [0002] DHA (docosahexaenoic acid) is a polyene fatty acid with a special structure (6 cis-configured double bonds in a non-conjugated system), which belongs to ω according to the position of the last double bond at its methyl end -3 series of typical long-chain highly unsaturated fatty acids. DHA in the human body is mainly distributed in the gray matter of the brain, the outer segment of retinal cells, the nervous system, breast milk, myocardium, eosinophilic leukocytes, sperm, etc., and its physiological functions include: inhibiting platelet aggregation; reducing neutral lipids in the blood; reducing extremely low High-density lipoprotein and low-density lipoprotein cholesterol and increase high-density lipoprotein cholesterol; reduce blood viscosity; nourish the brain and prevent Alzheimer's disease; improve vision and ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/12C12N15/01C12R1/89
Inventor 陈金卿陈俊煌吴美琼林超
Owner XIAMEN KINGDOMWAY GROUP
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com