Chikungunya virus isothermal amplification detection kit and primer thereof

Abstract

The invention provides a chikungunya virus isothermal amplification detection kit and a primer thereof. The kit comprises the primer, Bst DNA polymerase, a reaction liquid and SYBR Green I fluorescent dye, wherein the reaction liquid is 10mM deoxyribonucleoside triphosphate, 10*reaction buffer liquid and 150mM MaSO4; the sequences of the primer are sequences represented by SEQ ID NO:1-4 or sequences represented by SEQ ID NO:5-8 or sequences represented by SEQ ID NO:9-12 or sequences represented by SEQ ID NO:13-16. The kit provided by the invention has the advantages of short detection time, strong specificity, high sensitivity, high detection accuracy and programmed reaction, is easy and convenient to qualify and is widely applied to conventional detection and epidemiological survey in clinic and ports.

Description

technical field [0001] The invention relates to the field of molecular biology detection, in particular to a chikungunya virus isothermal amplification rapid detection kit and primers thereof. Background technique [0002] Chikungunya virus is the pathogen that causes chikungunya fever, which is mainly transmitted through the bite of Aedes mosquitoes. Chikungunya fever is a zoonotic disease, which is an acute viral infectious disease mainly characterized by fever, rash and joint pain. It is mainly prevalent in Africa and Southeast Asia. Areas with high vector density are prone to large-scale outbreaks and epidemics. Aedes mosquitoes are widely distributed in South China and Southwest my country, and become a potential cause of the outbreak of the disease. In 2006 alone, India reported more than 1.39 million suspected cases of chikungunya fever, and the incidence rate in some areas exceeded 45%. In 2008, the Guangdong Entry-Exit Inspection and Quarantine Bureau detected th...

AI Technical Summary

Problems solved by technology

Virus isolation is currently the gold standard for detection, but due to the long detection cycle of this technology, it is not conducive to the requirement of rapid detection; serum antibody detection can quickly diagnose chikungunya virus, but because the virus antibody is easy to detect Viruses produce cross-reactions, so the specificity is relatively low; although RT-PCR technology can solve the above problems well, it is not conducive to the requirements of on-site diagnosis and grass-roots detection due to the need to rely on relatively expensive instruments and equipment

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