Clostridium acetobutylicum for producing butanol by utilizing manihot as raw materials and application thereof

A kind of technology of Clostridium acetobutylicum and raw material, applied in the field of bioengineering

Inactive Publication Date: 2012-04-18
GUANGXI ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Examples

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Embodiment Construction

[0011] The methods in the following examples are conventional methods unless otherwise specified.

[0012] 1. Isolation and purification of Clostridium acetobutylicum

[0013] Ten soil samples of plant roots were collected from the suburbs of Nanning. Put the soil sample into a 10mL TYA medium test tube, the medium composition: 40 grams of glucose, 2 grams of beef extract, 2 grams of yeast extract, 6 grams of tryptone, 3 grams of ammonium acetate, 0.5 grams of potassium dihydrogen phosphate, seven After mixing 0.2 grams of magnesium sulfate water and 0.01 grams of ferric sulfate heptahydrate, they were heated in boiling water for 1 minute, and cultivated at a temperature of 37° C. in an anaerobic incubator. Observe regularly, pick out the test tubes with air bubbles, add 1.5% agar to the TYA medium, and isolate single colonies on the plate. A single colony was transferred to a medium containing 8% corn flour by mass for cultivation, and the strains with fast growth and gas p...

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Abstract

The invention discloses clostridium acetobutylicum for producing butanol by utilizing manihot as raw materials and application thereof. The clostridium acetobutylicum is preserved in China Center for Type CultureCollection with the number of CCTCC M 2011280. The clostridium acetobutylicum is capable of fermenting to produce the butanol, and particularly, the fermentation volume of the butanol in a fermentation medium taking the manihot as the raw materials can reach 13.07 g/L.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a Clostridium acetobutylicum which uses cassava as a raw material to produce butanol and its application in fermenting butanol. Background technique [0002] In chemical production, butanol is an important fine chemical raw material with a wide range of uses. It can be used not only as a solvent to produce plasticizers such as dibutyl phthalate and chemicals such as butyraldehyde, butyl phosphate, and butyl methacrylate, but also as an extraction agent for oils, medicines, and fragrances. In terms of liquid fuels that can replace petroleum, butanol is considered to be a new generation of liquid fuels that are suitable to replace petroleum due to its high energy density, low hydrophilicity, high octane number, and low volatility, which are far superior to ethanol. energy. [0003] The oil energy crisis has aroused widespread concern about the insufficient future energy supply in va...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P7/16C12R1/145
Inventor 庞浩裴建新左文朴刘春宇黄志民黄日波
Owner GUANGXI ACAD OF SCI
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