Preparation method and use method of dimethoate molecule imprinted polymer (MIP)

A technology of molecular imprinting and polymers, applied in material excitation analysis, fluorescence/phosphorescence, etc., can solve the problems of complex preparation process and poor accuracy, and achieve good selectivity, high sensitivity and accuracy, and strong operability Effect

Inactive Publication Date: 2012-05-02
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the existing molecularly imprinted polymers of organophosphorus pesticides have defects such as complicated preparation process and poor accuracy.

Method used

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  • Preparation method and use method of dimethoate molecule imprinted polymer (MIP)
  • Preparation method and use method of dimethoate molecule imprinted polymer (MIP)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1, a kind of preparation method of dimethoate molecularly imprinted polymer, comprises the following steps successively:

[0037] 1), using dimethoate as a template molecule, acrylamide as a functional monomer for polymerization, ethylene glycol dimethacrylate (EDMGA) as a crosslinking agent, chloroform as a porogen, and azobisiso Nitrile (AIBN) was used as the initiator.

[0038] Weigh 1.0 mmol of dimethoate and add it into a glass test tube containing 10 mL of chloroform, then add 4.0 mmol of acrylamide, and place the above reaction solution in a high-power (frequency range: 50 kHz) ultrasonic cleaner for ultrasonic treatment for 1 hour. So that the dimethoate molecule is fully combined with acrylamide, then add 40mmol of ethylene glycol dimethacrylate (EDMGA) as a cross-linking agent and 65mg of azobisisobutyronitrile (AIBN), then ultrasonically treat for 10 minutes at the same frequency minutes; a mixture was obtained.

[0039] 2) Put the above-mentione...

Embodiment 2

[0043]Example 2, the pretreatment of the analysis sample by the molecularly imprinted polymer of Delo fruit and the use of calcein-Pd 2+ For fluorescent probe detection dimethoate content, carry out the following steps successively:

[0044] 1) Accurately weigh 5.00 g of powdered tea samples into a 25 mL stoppered test tube, add 10 mL of methanol, mix well and ultrasonically (ultrasonic frequency is 40-60 kHz) at 40°C for 20 minutes; after standing for 10 minutes, Obtain the first supernatant, then centrifuge the first supernatant at 4000r / min for 5 minutes to obtain the second supernatant, and transfer the second supernatant into a 25mL stoppered test tube.

[0045] 2) Add 20 mg of dimethoate molecularly imprinted polymer (obtained in Example 1) to the above-mentioned secondary supernatant, and perform isothermal adsorption for 60 minutes in a 40° C. water bath thermostat. Centrifuge at a speed of 4000r / min for 5 minutes, discard the supernatant, and obtain a precipitate. T...

Embodiment 3

[0050] Embodiment 3, change the tea sample in embodiment 2 into another kind of tea sample, all the other are the same as embodiment 2; The obtained fluorescence intensity value is 75.94, 75.94-2.58=73.36, and 73.36 is substituted into formula y=2205.7x as y value In -41.34, get x=0.052mg / L; This is the dimethoate content in the solution after the test product is constant volume.

[0051] Since the solution to be tested has 10ml after constant volume, and the tea sample is 5.00g, the content of dimethoate in the tea sample obtained after conversion is 0.104mg / kg.

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Abstract

The invention discloses a preparation method of a dimethoate MIP, which comprises the following steps of: (1) mixing dimethoate as a template molecule, a functional monomer for polymerization, a cross-linking agent and pore former; (2) carrying out in-situ polymerization reaction of the mixture; (3) grinding the polymer, extracting and cleaning till the template molecule cannot be detected in the washed substance; and (4) drying the obtained MIP after the template molecule is removed in vacuum to obtain the dimethoate MIP. The invention further discloses a use method of the dimethoate MIP. The sample pre-treatment is carried out with a molecule imprinting technology. The content of dimethoate is measured by taking calcein-Pd2+ as a fluorescent probe through a fluorophotometry. The dimethoate MIP prepared by the method disclosed by the invention has higher adsorption rate on dimethoate, so that the interference of other substances on dimethoate is avoided.

Description

technical field [0001] The invention relates to a preparation method and application method of a dimethoate molecularly imprinted polymer. Background technique [0002] The application fields of molecularly imprinted polymers for organophosphorus pesticides include: 1), equipped in SPE columns, used in the sample pretreatment process of organophosphorus pesticide residue analysis; 2), assembled on sensors, and prepared suitable for organophosphorus pesticide A sensor device for detection and analysis; 3), assembled into a liquid chromatography packed column to prepare a liquid chromatography HPLC column suitable for the detection and analysis of organophosphorus pesticides. [0003] The current molecularly imprinted polymers for organophosphorus pesticides have defects such as complicated preparation process and poor accuracy. Contents of the invention [0004] The technical problem to be solved in the present invention is to provide a preparation method of dimethoate mol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F222/14C08F220/56C08F222/38C08F212/36C08F220/06C08F220/14C08F226/06C08J9/28C08J9/26G01N21/64
Inventor 沈生荣于海宁
Owner ZHEJIANG UNIV
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