In-vitro skin transcutaneous electrical resistance measuring device
A technology of resistance measurement and resistance measuring instrument, which is applied in the direction of measuring devices, measuring electrical variables, measuring resistance/reactance/impedance, etc., can solve problems such as unstable values, unfavorable readings, and lack of instruments, so as to improve measurement accuracy and cost. Inexpensive, easy-to-use effect
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specific Embodiment 1
[0035] Specific embodiment 1, when needing to measure the resistance value of isolated skin, its electrolytic solution can select the magnesium sulfate solution of 154mmol / L or 0.85% physiological saline, and its transcutaneous resistance measurement step is:
[0036] 1. Obtain an isolated skin with a diameter of about 20 mm through surgery, and fix it to the end of the supply pool 1 with a rubber "O" ring 3. The skin epidermis side 4 of the isolated skin faces into the supply pool 1, and the skin dermis side 5 toward the outside of the supply pool 1;
[0037] 2. The capacity of the inner electrolytic solution 7 injected into the supply pool 1 is 3 milliliters, the capacity of the outer electrolytic solution 6 injected into the receiving pool 2 is 10 milliliters, and the inner electrode 9 and the outer electrode 8 are fixed;
[0038] 3. Clamp the measuring wire crocodile clip 10 to the internal electrode 9 and the external electrode 8, turn on the transcutaneous resistance mea...
specific Embodiment 2
[0042] Specific embodiment 2, the percutaneous resistance measuring instrument 17 involved in this system is designed and manufactured by itself. In order to ensure the authenticity of the test results, the test results of the TH2816B precision LCR digital bridge (Changzhou Tonghui Electronics Co., Ltd.) were selected as the test results. Simultaneous comparison.
[0043] Eight normal skin slices from the same wistar rat were selected and fixed in the isolated skin fixation device, and the resistance value of the isolated skin was measured with the LCR digital bridge and the transcutaneous electrical resistance measuring instrument 17 respectively, and the results are shown in Table 2 :
[0044]
[0045] Note: *P>0.05, there is no statistically significant difference between the two groups.
[0046] By comparison, it can be seen that the test result of the transcutaneous resistance measuring instrument 17 is consistent with the test result of the LCR digital bridge, indica...
specific Embodiment 3
[0047] Specific embodiment 3, by Figure 5 to Figure 6 It can be seen that the isolated skin fixation device is used to evaluate the maintenance of the barrier function of the isolated skin stored in vitro for 24 hours. Select 8 pieces of normal isolated skin from the same wistar rat and fix them in the isolated skin fixation device, measure and record 0, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 5, 6, 8, 10 , 12, 16, 20, 24 and 28h, a total of 18 time points of transcutaneous resistance value, and at the same time, the isolated skin of 2, 4, 8, 20 and 24h and fresh normal rat skin were made to make case slices for HE staining observation . The above evaluation was repeated 3 times. Its evaluation results are as Figure 5 ,Depend on Figure 5 The evaluation results showed that the transcutaneous resistance value of the isolated skin increased slightly within 2 hours after being fixed on the isolated skin fixation device, and the fluctuation of the transcutaneous resistance value bet...
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