Compositions and methods for improved saccharification of biomass

A biomass, carbohydrate technology, applied in the field of compositions and methods for improved saccharification of biomass, can solve problems such as increasing fermentation costs

Inactive Publication Date: 2012-05-23
QTEROS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This adds to the cost of fermentation since hydrolysis must be done by adding an expensive mixture of enzymes that can hydrolyze 5- and 6-carbon polysaccharides

Method used

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  • Compositions and methods for improved saccharification of biomass
  • Compositions and methods for improved saccharification of biomass
  • Compositions and methods for improved saccharification of biomass

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0198] Large-scale production of fermentation end products from biomass

[0199] In one aspect, microbial organisms such as C. phytofermentans or Clostridium sp. Q.D are used for large-scale production of fermentation end products (eg, ethanol) from biomass. In one embodiment, one first hydrolyzes a biomass material including high molecular weight carbohydrates to low molecular weight carbohydrates, and then uses microbial cells to ferment the low molecular weight carbohydrates to produce ethanol. In another embodiment, one ferments the biomass material itself without chemical and / or enzymatic pretreatment. In the first method, acids (such as Bronsted acids, such as sulfuric acid or hydrochloric acid), alkalis (such as sodium hydroxide), heat treatment, steam explosion, ammonia fiber expansion ("AFEX"), lime method, enzymes or their combined to complete the hydrolysis. Hydrogen and other products of fermentation can be captured and purified if desired, or disposed of by, for...

Embodiment 1

[0250] Example 1. Adding cellulase

[0251] To study the effect of supplementing exogenous enzymes, a mixture of hydrolytic enzymes and individual hydrolytic enzymes were added during the fermentation of corn stover biomass.

[0252] The following operating conditions and processing parameters were then implemented for C. phytofermentans for fermentation of NaOH-pretreated corn stover with enhanced enzymes in 250 ml shake flasks containing 100 ml of medium (Table 3).

[0253] table 3

[0254]

[0255]

[0256] Seed Propagation Medium (QM1) Formula:

[0257]

[0258]

[0259]

[0260] Seed propagation medium was prepared according to the method described above. Degas basal medium, salts, and substrates with nitrogen, then autoclave. After sterilization, combine 94ml of basal medium with 1ml of 100x salts and 5ml of 20x substrate to give the final concentration. All supplements were prepared under anaerobic and sterile conditions.

[0261] Fermentation me...

Embodiment 2

[0282] Example 2. Modification of microorganisms

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Abstract

Compositions and methods are provided for enhancing saccharification of biomass with one or more enzymes to enhance availability of substrates for fermentation by a microorganism. Microorganisms are also modified to enhance activity of one or more hydrolytic enzymes that are present endogenously in or are introduced heterologously into a host microorganism.

Description

[0001] claim priority [0002] This application claims priority to U.S. Provisional Patent Application Serial No. 61 / 221,523, filed June 29, 2009, and U.S. Nonprovisional Patent Application Serial No. 12 / 630,784, filed December 3, 2009, which The application is hereby incorporated by reference in its entirety. [0003] sequence listing [0004] This application includes a Sequence Listing that has been submitted via EFS-Web and is hereby incorporated by reference in its entirety. Said ASCII copy, created on November 30, 2009, is named 37836717.txt and is 81,443 bytes in size. Background technique [0005] Biomass is a renewable energy source that can be biologically fermented to produce end products such as fuels (eg alcohols, ethanol, organic acids, acetic acid, lactic acid, methane or hydrogen). Biomass includes agricultural residues (corn stalks, pasture, straw, grain husks, bagasse, etc.), animal waste (from manure from cattle, poultry, and pigs), wood (tree or bark, sa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/04C12P7/10C12P7/08C12P7/06C12P7/56C12P7/54C12P7/40C12N1/21C12N15/74C12R1/145
CPCC12P7/54C12P7/10Y02E50/17C12P7/04Y02E50/16C12P19/14C12P7/40C12P7/56Y02E50/10
Inventor W·G·莱图夫M·施马利施K·黄M·A·阿里
Owner QTEROS INC
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