Therapeutic gene for anaerobic tissue targeting delivery and selectivity stabilization expression method and its application

A stable expression and selective technology, used in gene therapy, drug combinations, pharmaceutical formulations, etc., can solve the problems of low expression level, easy loss and non-existence of therapeutic genes, and achieve the effect of reducing in vivo toxicity and avoiding expression.
CN102477440AInactive Publication Date: 2012-05-30NANJING UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NANJING UNIV
Publication Date
2012-05-30
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention belongs to biology technical field, concretely relates to a method for realizing a therapeutic gene on anaerobic tissue targeting delivery and selectivity stabilization expression. According to the invention, a prokaryotic bacteria nitroso reductase promoter PnirB induced by prokaryotes anaerobe is taken as composition of a therapeutic gene promoter, anaerobic targeting bacteria, low copy plasmid and the like, so that therapeutic gene enables an specific expression under low oxygen or hypoxia environment in vivo and in vitro. The method for realizing the therapeutic gene on the anaerobic tissue targeting delivery and selectivity stabilization expression is effectively treating the anaerobic tissue diseases containing tumor, or treating the anaerobic tissue diseases comprising tumor by combining other chemical medicaments and traditional Chinese medicines, and can be used for preparing antitumor drugs.
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Description

Technical field

[0001] The invention belongs to the field of genetic engineering biotechnology, and specifically relates to methods and applications for targeted delivery and selective stable expression of therapeutic genes in anaerobic tissues. Background technique

[0002] Tumor gene therapy can fundamentally compensate for the abnormality of gene expression caused by the occurrence and development of tumors and correct the disorder of tumors at the gene level, so it has broad application prospects. However, there are still some bottlenecks in tumor gene therapy. For example, in the case of systemic drug delivery, how to make the transferred therapeutic gene be restricted to high-level and high-specific expression within the solid tumor or the entire tumor environment. Is it not expressed in other normal tissues to effectively eliminate or reduce the possible side effects of therapeutic genes? This technical difficulty limits the practical application of gene therapy in the tr...

Claims

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