Preparation method of euphausia superba protein peptide mixture

Abstract

Relating to preparation of euphausia protein peptide mixtures, the invention provides a method for preparing a high-quality low-fluorine euphausia superba protein polypeptide mixture. By adopting euphausia superba for freezing and homogenate, the method of the invention is characterized by: adding a papain preparation with a mass ratio of 1%-2% into a homogenate liquid, mixing them well, and leaving them to 3h-5h of reaction under a stirring temperature of 50-60DEG C, then conducting centrifugation to obtain a liquid phase A and a sediment B; adding the sediment B into isopyknic water for dissolving so as to obtain a suspending solution, which is then filtered by a bolting silk of 300 meshes, thus obtaining a suspending solution C and a residue D, washing the residue D once with water so as to obtain a residue E and a suspending solution F, combing the suspending solution C and the suspending solution F and carrying out centrifugation, thus obtaining a liquid phase G and a sediment H,and subjecting the liquid phase A, the liquid phase G and the sediment H to drying so as to obtain a low-fluorine euphausia protein peptide mixture dry basis.

Description

technical field [0001] The invention relates to a method for preparing a large krill protein peptide mixture. Background technique [0002] Antarctic krill (Euphausia superba) is an important marine biological resource. The crude protein content of naturally grown Antarctic krill muscle dry sample is 64.44%, the crude fat content is 5.14%, the ash content is 10.90%, and the nitrogen-free extract content is 19.52%; the total amount of 18 kinds of amino acids is 57.16g / 100g, of which 8 kinds The essential amino acid content of the human body is 25.88g / 100g, accounting for 45.25% of the total amino acid; the essential amino acid index (EAAI) is 54.94%, and its composition ratio meets the FAD / WHO standard; the content of four kinds of umami amino acids is 23.06g / 100g, accounting for 35.98% of the total amount of amino acids. Krill has millions of tons of production and high-quality protein in the ocean. It is known as the largest animal protein bank on the earth and has great ...

AI Technical Summary

Problems solved by technology

At present, there are few researches on the defluorination of Antarctic krill before eating and the defluorination of Antarctic krill during protein extraction. There is only a patent application number 200910019197.2 that is still in the publication stage in China. The technical solution described in this patent application is to integrate Antarctic krill Krill is frozen, crushed, protein is extracted under alkaline conditions, protein is precipitated and separated under acidic conditions, and defluoridation treatment is carried out by pickling. However, the method of acid-base extraction of protein has severe reaction conditions and has a certain reverse damage effect on protein. , so as to have a certain impact on the quality of the prepared protein, and the method of acid-base extraction of protein needs to consume a lot of water resources to wash away the acid and alkali, and consumes a lot of energy; a method of electrode enrichment is used abroad to reduce The fluorine content of Antarctic krill, the results show that after 120 minutes of treatment with an aluminum electrode, the fluorine content of live krill reaches 95ppm and 15ppm respectively, but this treatment method requires special The electrode is not conducive to industrial mass production