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Preparation method of pueraria root flavone extract and its product

A technology of kudzu root flavonoids and extracts, which is applied in the field of preparation of kudzu root flavonoids extracts, can solve the problems of unfavorable large-scale production, low extraction efficiency, cumbersome steps, etc., and achieve the effect of simple operation, high extraction efficiency and few process steps

Inactive Publication Date: 2012-06-13
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many methods for extracting Pueraria flavonoids, but the process is complicated, the steps are cumbersome, the extraction efficiency is low, and the cost is high, which is not conducive to large-scale production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The preparation method of kudzu root flavonoids extract, concrete steps are as follows:

[0032] a. Preprocessing

[0033] Wash the kudzu root, homogenate, filter, and collect the filtrate and filter residue respectively;

[0034] b. to starch

[0035] The filtrate obtained in step a was left to stand for 24 hours, the supernatant was collected, and the supernatant was combined with the filter residue obtained in step a to obtain a crude extract;

[0036] c. Sterilization

[0037] The crude extract obtained in step b is sterilized at 70°C for 120 minutes, and then cooled to 20°C;

[0038] d. Hydrolysis

[0039] Add 1% amylase, 2% glucosidase and 2% cellulase corresponding to the volume of the sterilizing solution to the sterilizing solution obtained in step c, hydrolyze for 24 hours, filter, collect the filtrate, and obtain the hydrolyzed solution;

[0040] e. Concentration

[0041] Concentrate the hydrolyzate obtained in step c under reduced pressure, and spray d...

Embodiment 2

[0044] The preparation method of kudzu root flavonoids extract, concrete steps are as follows:

[0045] a. Preprocessing

[0046] Wash the kudzu root, add water equivalent to 5 times the weight of the kudzu root, homogenate, filter, and collect the filtrate and filter residue respectively;

[0047] b. In addition to starch

[0048] Centrifuge the filtrate obtained in step a at a rotational speed of 2000 rpm for 30 minutes, collect the supernatant, and combine the supernatant with the filter residue obtained in step a to obtain a crude extract;

[0049] c. Sterilization

[0050] The crude extract obtained in step b is sterilized at a temperature of 110°C for 5 minutes, and then cooled to 50°C;

[0051] d. Hydrolysis

[0052] Add the sterilizing solution obtained in step c to glycosidase equivalent to 0.01% of the volume of the sterilizing solution, hydrolyze for 12 hours, filter, collect the filtrate, and obtain the hydrolyzate;

[0053] e. Concentration

[0054] The hydr...

Embodiment 3

[0057] The preparation method of kudzu root flavonoids extract, concrete steps are as follows:

[0058] a. Preprocessing

[0059] Wash the kudzu root, add water equivalent to 2 times the weight of the kudzu root, homogenize, filter, and collect the filtrate and filter residue respectively;

[0060] b. In addition to starch

[0061] Centrifuge the filtrate obtained in step a at a rotational speed of 3000 rpm for 10 minutes, collect the supernatant, and combine the supernatant with the filter residue obtained in step a to obtain a crude extract;

[0062] c. Sterilization

[0063] The crude extract obtained in step b is sterilized at a temperature of 90°C for 20 minutes, and then cooled to 35°C;

[0064] d. Hydrolysis

[0065] Add amylase equivalent to 0.1% of the volume of the sterilized solution to the sterilized solution obtained in step c, hydrolyze for 12 hours, filter, collect the filtrate, and obtain a hydrolyzed solution;

[0066] e. Concentration

[0067] The hydro...

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PUM

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Abstract

The invention discloses a preparation method of a pueraria root flavone extract. The method for preparing a pueraria root flavone extract comprises the steps of pretreatment, starch removal, sterilization, hydrolysis, and concentration, etc. The preparation method has the advantages of simple process, low cost, and high extraction efficiency. The invention also discloses the pueraria root flavoneextract prepared by the preparation method. With high flavone content, the extract in the invention can be used for preparing medicaments for lowering blood pressure, reducing blood sugar, decreasingblood lipid, and beauty maintaining, etc., and has broad application prospects.

Description

technical field [0001] The invention belongs to the field of pharmacy, and in particular relates to a preparation method of kudzu root flavonoid extract and products thereof. Background technique [0002] Pueraria lobata is a perennial vine, cool in nature, sweet and pungent in taste. It has the effects of clearing heat, reducing fire, promoting body fluid, clearing rash, raising yang, stopping diarrhea, and detoxifying. For exogenous fever and headache, high blood pressure, thirst, diabetes, impermeable measles, febrile dysentery, and diarrhea. The flavonoids in kudzu root have a certain effect on hypertension, hyperlipidemia, hyperglycemia and cardiovascular and cerebrovascular diseases. At present, there are many methods for extracting Pueraria flavonoids, but the processes are complex, the steps are cumbersome, the extraction efficiency is low, and the cost is high, which is not conducive to large-scale production. [0003] Therefore, there is an urgent need for a pre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/48A61P3/06A61P9/00A61P9/12
Inventor 李学刚袁吕江叶小利
Owner SOUTHWEST UNIV
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