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Method for identifying sugar-doped dried sea cucumber

A technology of dried sea cucumbers and sea cucumbers, which is applied in the field of identification of sugar-mixed dried sea cucumbers, can solve problems such as interference of test results, complicated processing process, and interference of results, and achieve the effects of good stability, simple operation, and high accuracy

Inactive Publication Date: 2012-06-13
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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AI Technical Summary

Problems solved by technology

The report of existing measuring sugar content method at present, as the application number is 200910037365.0 " the method for quickly measuring pentose and hexose content in the hemicellulose extract liquid ", is to adopt dual-wavelength method to measure total sugar, pentose and hexose content simultaneously, That is, it is suitable for the determination of monosaccharide content, and it is suitable for the samples to be tested with clear ingredients. However, the processing process of "dried sea cucumber mixed with sugar" is complicated, and the sugar components mixed in are various, so this method cannot be used to determine the content of sugar mixed in dried sea cucumbers. Sugar; Determination of the total sugar content in the national standard GB / T 15672-2009 of the People's Republic of China and GB / T 9695.31-2008 Determination of the total sugar content of meat products all use the spectrophotometric method (phenol sulfuric acid method) to determine the total sugar content in food. The sugar content has a good reference value, but the methods in the standard are respectively aimed at the determination of the total sugar content in edible fungi and meat products, and are not suitable for the analysis of exogenous total sugar content in sea cucumbers, and cannot meet the requirements for identifying sugar-admixed sea cucumbers
This is because sea cucumber itself has a high protein content and a large amount of polysaccharides, and soluble proteins and polysaccharides will interfere with the test results
The determination of the total sugar content in edible fungi will hydrolyze the polysaccharides of dried sea cucumbers and measure them in the form of added sugars, resulting in misjudgment of the determination results
However, the determination of the total sugar content in meat products has insufficient extraction effect, and water is used as the extraction solvent, and the soluble protein in it will interfere with the results.

Method used

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  • Method for identifying sugar-doped dried sea cucumber

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Embodiment 1: the mensuration of total sugar content in salt-dried sea cucumber

[0028] The samples were pulverized with a pulverizer and passed through a 40-mesh sieve. Put the sample in a ziplock bag and put it in a desiccator for later use. Weigh about 1g of the sample, accurate to 0.001g, carefully pour it into a 100mL conical flask, add 50mL of 60% ethanol aqueous solution, the volume ratio of the dried sea cucumber sample to the ethanol aqueous solution is 1:50, shake and extract in a water bath at 30°C for 1 hour, and cool to After room temperature, all the samples and extracts were transferred to a 100mL volumetric flask, rinsed twice, constant volume, filtered, and the filtrate was set aside.

[0029] Accurately draw 1 mL of the above-mentioned filtrate, add 1 mL of 5% phenol aqueous solution, mix well, then quickly add 5 mL of concentrated sulfuric acid vertically, let it stand for 10 min, keep it in a water bath at 30°C for 20 min, and measure the reaction ...

Embodiment 2

[0038] Embodiment 2: the mensuration of total sugar content in dry sea cucumber

[0039]The sample was pulverized with a pulverizer, passed through a 40-mesh sieve, put the sample in a ziplock bag, and put it in a desiccator for later use. Weigh about 1g of the sample, accurate to 0.001g, carefully pour it into a 100mL conical flask, add 50mL of 60% ethanol aqueous solution, shake and extract in a water bath at 30°C for 1h, cool to room temperature, transfer all the sample and extract to a 100mL volumetric flask, Rinse twice, constant volume, filter, and the filtrate is set aside.

[0040] Accurately draw 1 mL of the above-mentioned filtrate, add 1 mL of 5% phenol aqueous solution, mix well, then quickly add 5 mL of concentrated sulfuric acid vertically, let it stand for 10 min, keep it in a water bath at 30°C for 20 min, and measure the reaction solution at 490 nm (Specrd S100 UV-Vis Spectrophotometer, Analytik Jena Company, Germany). meter) to measure the absorbance. Obtai...

Embodiment 3

[0041] Example 3: Determination of Exogenous Total Sugar Content in Sugar-Added Dried Sea Cucumber

[0042] The sample was pulverized with a pulverizer, passed through a 40-mesh sieve, put the sample in a ziplock bag, and put it in a desiccator for later use. Weigh about 1g of the sample, accurate to 0.001g, carefully pour it into a 100mL conical flask, add 50mL of 60% ethanol aqueous solution, shake and extract in a water bath at 30°C for 1h, cool to room temperature, transfer all the sample and extract to a 100mL volumetric flask, Rinse twice, make up to volume, and filter; in order to prevent the concentration of sugar in the filtrate from being too high, accurately draw 10 mL of the filtrate, transfer it to a 250 mL volumetric flask, make up to volume, and use the filtrate for later use.

[0043] Accurately draw 1 mL of the above-mentioned filtrate, add 1 mL of 5% phenol aqueous solution, mix well, then quickly add 5 mL of concentrated sulfuric acid vertically, let it stan...

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Abstract

The invention discloses a method for identifying a sugar-doped dried sea cucumber. The method is characterized by comprising the following steps of: vibrating and extracting exogenous sugar in the sea cucumber in a water bath at certain temperature by using an aqueous solution of ethanol at high concentration, wherein polysaccharide in the dried sea cucumber cannot be extracted, so that endogenous sugar and the exogenous sugar in the dried sea cucumber can be identified well; and measuring the total content of the extracted sugar by using an optimized phonel-sulfate method. The method is easyto operate, and high in stability and high in accuracy, and is suitable for measuring the total content of the exogenous sugar in the dried sea cucumber; and by the method, the sugar-doped dried sea cucumber can be effectively identified.

Description

Technical field: [0001] The invention relates to the field of quality identification of aquatic products, in particular to a method for identifying dried sugar-mixed sea cucumbers. Background technique: [0002] Sea cucumber is a nutritious food with high protein content and balanced amino acids and trace elements. It contains about 76.5g of protein, 1.1g of fat, 13.2g of polysaccharide-based sugars, calcium, phosphorus and iron per 100g of pure dry product. , iodine and other beneficial minerals 4.2g. In addition, sea cucumbers also have high medicinal value. According to medical records, sea cucumbers can nourish the kidney and replenish essence, and have certain curative effects on the treatment of tuberculosis hemoptysis, aplastic anemia, diabetes, etc., and have been favored by people for a long time. In the history of our country, sea cucumber is one of the eight treasures of marine products that are as famous as bird's nest and shark's fin. [0003] However, due to ...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N1/28
Inventor 王联珠殷邦忠李烨江艳华姚琳朱文嘉宋春丽郭萌萌谭志军李晓庆
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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