Method for electrochemically and rapidly detecting dopamine in blood

Abstract

The invention discloses a method for electrochemically and rapidly detecting dopamine in blood and relates to the technical field of clinical medical detection, in particular to a method for detecting the dopamine in the blood. In the method, an electrochemical method is adopted; a glassy carbon electrode for modifying multi-walled carbon nanotubes / choline is used as a working electrode; the dopamine is dissolved in a neutral phosphoric acid buffer solution; circulating voltammetry scanning is carried out in a potential range of -0.4V to 0.8V; according to an obtained electrochemical curve, the content of the dopamine in the blood is subjected to primary qualitative analysis and quantitative analysis; an observation result that a corresponding oxidation reduction peak occurs under the voltage of about 0.2V on a circulating voltammetry curve proves that a blood sample contains the dopamine; and an observation result that the corresponding oxidation reduction peak does not occur under the voltage of about 0.2V on the circulating voltammetry curve proves that the blood sample does not contain the dopamine. Therefore, according to the method, the blood sample is primarily identified whether to contain the dopamine only in 1 to 10 minutes. The method is rapid, accurate and convenient.

Description

technical field [0001] The invention relates to the technical field of clinical medical detection, in particular to a method for detecting dopamine in blood. Background technique [0002] Dopamine is a small molecular substance that exists in the living body. Its detection has important medical reference value. [0003] Dopamine (DA) is a very important neurotransmitter in mammals, used to help cells deliver impulse chemicals. It is widely distributed in the central nervous system, midbrain and body fluids. It is mainly responsible for the feelings and sensations of the brain, conveying information of excitement and happiness, and is also related to addiction. Both smoking and drug use can increase the secretion of dopamine, which makes addicts feel happy and excited. Insufficient dopamine can cause people to lose the ability to control muscles, and in severe cases, people's hands and feet can vibrate involuntarily, leading to Parkinson's disease, or losing the ability t...

AI Technical Summary

Problems solved by technology

However, the adsorption of dopamine oxidation products will contaminate the electrode, resulting in poor selectivity and reproducibility of electrochemical determination of dopamine

[0005] However, when various substrate electrodes such as carbon materials and noble metal materials are used as electrochemical detectors, the electrochemical detection of dopamine is often interfered by the coexisting components of ascorbic acid and uric acid in the body.

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