Method for preparing egg yolk antibody, egg yolk antibody prepared by method and application of egg yolk antibody
A yolk antibody, volume technology, applied in the direction of antibodies, antiviral agents, antiviral immunoglobulins, etc., can solve the problems of poor cure effect of severely ill bee colonies, inability of bees to eat well, and inability to directly kill viruses, etc. To achieve the effect of no drug residue, not easy to fail, and rapid action
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[0022] Example 1
[0023] 1. Preparation of inactivated vaccine with oil adjuvant of sac-like larvae
[0024] The diseased larvae tested positive by RT-PCR were mixed with pH 7.6 phosphate buffer solution first, and 1 ml of phosphate buffer solution was added for every 1g of diseased larvae. The ground mixture was added with chloroform, and the volume of the above mixture Ratio 1:0.8, homogenize at 37°C for 10 min, then centrifuge at 1000 rpm for 10 min, collect the supernatant and precipitate respectively; take the supernatant, add chloroform with a volume ratio of 1:0.8 to the supernatant, Shake in a 37℃ water bath for 10 min, and then centrifuge at 1000 rpm for 10 min. Collect the supernatant and the precipitate. The supernatant is treated twice with this method to collect the supernatant and the precipitate; Collect them together, dissolve with pH 7.6 phosphate buffer, add 1ml phosphate buffer per 1g of precipitate, then centrifuge at 1000 rpm for 10 min, take the supernatant,...
Example Embodiment
[0030] Example 2
[0031] 1. Preparation of inactivated vaccine with oil adjuvant of sac-like larvae virus
[0032] The diseased larvae tested positive by RT-PCR were mixed with pH 7.4 phosphate buffer solution first, and 1.5ml of phosphate buffer solution was added to every 1g diseased larvae. The ground mixture was added with chloroform, and the mixture The volume ratio is 1:1.2, homogenize at 39°C for 8 min, then centrifuge at 1200 rpm for 8 min, and collect the supernatant and sediment respectively; take the supernatant and add chloroform with a volume ratio of 1:1.2 to the supernatant , Shake in a water bath at 39℃ for 8 min, then centrifuge at 1200 rpm for 8 min, collect the supernatant and the precipitate, continue to process the supernatant three times, collect the supernatant and the precipitate; Collect them together, dissolve with pH7.4 phosphate buffer, add 1.5ml phosphate buffer per 1g of precipitate, then centrifuge at 1200 rpm for 8 min, take the supernatant, and mi...
Example Embodiment
[0038] Example 3
[0039] 1. Preparation of inactivated vaccine with oil adjuvant of sac-like larvae
[0040] The diseased larvae tested positive by RT-PCR were mixed with a pH 7.8 phosphate buffer solution. Add 2ml of phosphate buffer solution for every 1g of diseased larvae. Add chloroform to the ground mixture, and the volume of the above mixture Ratio 1:1.5, homogenize at 40°C for 9 min, then centrifuge at 800 rpm for 9 min, collect the supernatant and precipitate respectively; take the supernatant, add chloroform with a volume ratio of 1:1.5 to the supernatant, Shake in a 40℃ water bath for 9 minutes, and then centrifuge at 800 rpm for 9 minutes. Collect the supernatant and the precipitate. The supernatant is processed by this method four times, and the supernatant and the precipitate are collected; the resulting precipitate is collected Together, dissolve with pH 7.8 phosphate buffer, add 2ml phosphate buffer per 1g of precipitate, then centrifuge at 800 rpm for 9 min, take ...
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