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Method for preparing egg yolk antibody, egg yolk antibody prepared by method and application of egg yolk antibody

A yolk antibody, volume technology, applied in the direction of antibodies, antiviral agents, antiviral immunoglobulins, etc., can solve the problems of poor cure effect of severely ill bee colonies, inability of bees to eat well, and inability to directly kill viruses, etc. To achieve the effect of no drug residue, not easy to fail, and rapid action

Active Publication Date: 2012-06-20
LIAONING YIKANG BIOLOGICAL CORP LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, although there are traditional Chinese medicines used for the treatment of bee cystic larvae, the traditional Chinese medicine treatment is mainly through immune regulation, indirect anti-virus, and can not directly kill the virus, and the effect is relatively slow, especially for severely ill bee colonies. At the same time, due to the smell of traditional Chinese medicine, the bees cannot eat well, and even escape

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Preparation of inactivated vaccine with oil adjuvant of Chinese bee cystic larvae virus

[0024] The diseased larvae detected positive by RT-PCR were mixed with the phosphate buffer solution of pH 7.6 first, and 1ml of phosphate buffer solution was added to each 1g of diseased larvae, and chloroform was added to the ground mixed solution, and the volume of the above mixed solution Ratio 1:0.8, homogenate at 37°C for 10 min, then centrifuge at 1000 rpm for 10 min, collect the supernatant and sediment respectively; take the supernatant, add chloroform with a volume ratio of 1:0.8 to the supernatant, Shake in a water bath at 37°C for 10 min, then centrifuge at 1000 rpm for 10 min, collect the supernatant and sediment respectively, continue to treat the supernatant twice with this method, and collect the supernatant and sediment respectively; Collect together, dissolve with pH 7.6 phosphate buffer, add 1ml phosphate buffer per 1g of precipitate, then centrifuge at 1000 r...

Embodiment 2

[0031] 1. Preparation of inactivated vaccine with oil adjuvant of Chinese bee cystic larvae virus

[0032]The diseased larvae detected positive by RT-PCR were first mixed with the phosphate buffer solution of pH7.4, and 1.5 ml of phosphate buffer solution was added to every 1 g of diseased larvae, and chloroform was added to the mixed solution after grinding, and mixed with the above mixed solution Volume ratio 1:1.2, homogenate at 39°C for 8 minutes, then centrifuge at 1200 rpm for 8 minutes, collect the supernatant and sediment respectively; take the supernatant, add chloroform with a volume ratio of 1:1.2 to the supernatant , shake in a water bath at 39°C for 8 min, then centrifuge at 1200 rpm for 8 min, collect the supernatant and precipitate respectively, continue to treat the supernatant three times with this method, and collect the supernatant and precipitate respectively; Collect together, dissolve with pH7.4 phosphate buffer, add 1.5ml phosphate buffer per 1g of preci...

Embodiment 3

[0039] 1. Preparation of inactivated vaccine with oil adjuvant of Chinese bee cystic larvae virus

[0040] The diseased larvae detected positive by RT-PCR were first mixed with the phosphate buffer solution of pH7.8, and 2ml of phosphate buffer solution was added to each 1g of diseased larvae, and chloroform was added to the ground mixed solution, and the volume of the above mixed solution Ratio 1:1.5, homogenate at 40°C for 9 minutes, then centrifuge at 800 rpm for 9 minutes, collect the supernatant and sediment respectively; take the supernatant, add chloroform with a volume ratio of 1:1.5 to the supernatant, Shake in a water bath at 40°C for 9 minutes, and then centrifuge at 800 rpm for 9 minutes to collect the supernatant and sediment respectively. The supernatant continues to be treated by this method four times, and the supernatant and sediment are collected respectively; the resulting sediment is collected Together, dissolve with pH7.8 phosphate buffer, add 2ml of phosp...

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Abstract

The invention discloses a method for preparing an egg yolk antibody, the egg yolk antibody prepared by the method and the application of the egg yolk antibody. According to the technical scheme, the method comprises the following steps of: multiplying viruses by using an apis cerana smith saccular larva as a virus multiplication model; preparing an apis cerana smith saccular larva virus oil adjuvant inactivated vaccine by using the multiplied viruses; continuously immunizing a laying hen for three times by using the prepared inactivated vaccine; measuring the titer of the antibody by an indirect enzyme-linked immune sorbent assay (ELESA); collecting eggs when the titer reaches more than 28; and purifying the egg yolk antibody by a hydration method. The egg yolk antibody is used for treating wasp swarms infected by apis cerana smith saccular larva viruses, has the recovery rate of more than 96.25 percent, is convenient to use, has high specificity and quick response, and does not have relapse and medicinal residues.

Description

technical field [0001] The invention relates to a preparation method of egg yolk antibody, the egg yolk antibody prepared by the method and application thereof. Background technique [0002] Apis chinensis has been included in the national livestock and poultry genetic resources protection list, and it is also one of the top ten livestock and poultry genetic resources protection species in Liaoning Province. It is a precious local bee species resource in my country. Raising bees has also become an effective way for farmers in mountainous areas to make a fortune . Chinese bees play an irreplaceable role in maintaining the plant ecosystem in my country. Studies have shown that once Chinese bees go extinct, 30% of the plants in my country will become extinct. At present, due to the reduction in production of Chinese bees, some national-level secondary protected plants are on the verge of extinction. In recent years, in order to strengthen the development of Chinese bee industry...

Claims

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Application Information

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IPC IPC(8): C07K16/10C07K16/02A61K39/42A61P31/14
Inventor 马鸣潇张大利王立辛袁小波宋英今和绍禹费东亮韩喜彬徐晓丽
Owner LIAONING YIKANG BIOLOGICAL CORP LTD
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