Method for preparing prednisolone
A prednisolone, prednisone technique
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Embodiment 1
[0032] A kind of production method of prednisolone, the step of its production method is:
[0033] (1) First-level seed culture: use Candida utilis CGMCC 2.1180 as the production strain to connect a full ring from the inclined plane to 30mL seed medium, and place it on a rotary shaker with a speed of 160r / min and a temperature of 30 Cultivate for 22 hours under the condition of ℃ to obtain seed culture solution suitable for inoculation.
[0034] (2) Secondary fermentation culture: insert the seed culture liquid into the fermentation medium according to the inoculation amount of 5% volume ratio, control the shaking table speed 180r / min during the fermentation process, and cultivate it under the temperature condition of 30 ℃ for 24 hours, Obtain bacterial cell fermentation broth.
[0035] (3) Transformation: pulverize prednisone and grind it finely, with a particle size of 14 μm, put it into the bacterial fermentation liquid under aseptic conditions, and the feeding amount is 5...
Embodiment 2
[0038] (1) First-level seed culture: connect Candida utilis CGMCC 2.1180 from a slant to a 30mL seed medium, and cultivate it at 32°C for 18 hours on a rotary shaker at 180r / min , to obtain a seed culture solution suitable for inoculation;
[0039] (2) Secondary fermentation culture: insert the well-grown seed culture solution into 30mL fermentation medium at an inoculum size of 3% (v / v); control the shaking table speed 170r / min during the fermentation process, and cultivate at 30°C After 22 hours, the bacterial cell fermentation liquid was obtained;
[0040] (3) Transformation: Grinding prednisone finely, the particle size is 15 μm, put it into the bacterial fermentation liquid under aseptic conditions, and the feeding amount is 6g / L; then add hydrophilic organic solvent 3 in the bacterial fermentation liquid %(v / v) ethanol solubilization; Control the shaker speed 190r / min in the transformation process, transform 40 hours under the temperature condition of 35 ℃, after transf...
Embodiment 3
[0043] (1) Primary seed culture: connect Candida utilis CGMCC 2.1180 from a slant to a 30mL seed culture medium, and cultivate it at 30°C for 20 hours on a rotary shaker at 180r / min , to obtain a seed culture solution suitable for inoculation;
[0044] (2) Secondary fermentation culture: insert the well-grown seed culture solution into 30mL fermentation medium at an inoculum size of 4% (v / v); control the shaking table speed 160r / min during the fermentation process, and cultivate at 33°C After 18 hours, the bacterial cell fermentation liquid was obtained;
[0045](3) Transformation: Prednisone is pulverized and ground finely, and the particle size is 16 μm, and it is put into the thalline fermentation broth under aseptic conditions, and the feeding amount is 8g / L; then in the thallus fermentation broth, add hydrophilic organic solvent 5 %(v / v) dimethyl sulfoxide solubilization, control the shaker speed 200r / min during the conversion process, convert 60 hours under the temperat...
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