Method for rapidly determining total antioxidant capacity of marine bivalves

Abstract

The invention relates to a method for determining antioxidant capacity of ocean shellfishes, in particular to a method for rapidly determining total antioxidant capacity of marine bivalves, which replaces a conventional spectrophotometer with a full wave frequency scanning type microplate reader a 96 ferment plate for determining total antioxidant capacity of processed samples, can determine batches of samples rapidly and accurately in a short time and accordingly can detect interspecies resistance study of the marine bivalves rapidly. The method rapidly determining total antioxidant capacity of marine bivalves can determine the total antioxidant capacity of the marine bivalves with different varieties and different textures in batch mode, and determination speed is improved by about 10 times compared with a conventional method.

Description

technical field [0001] The invention relates to a method for measuring the antioxidant capacity of marine shellfish, in particular to a method for quickly measuring the total antioxidant capacity of marine bivalve shellfish. Background technique [0002] Total antioxidant capacity (TAC) is a comprehensive index to measure the ability of animals to remove excess reactive oxygen species (Reactive oxygen radical species, ROS) in the body. In the state of disease and stress, the oxygen demand of the animal body will increase sharply, and the metabolism will be abnormal, causing oxidative stress, and a large amount of ROS will be produced and accumulated in the cells. ROS reacts with proteins, nucleic acids and lipids to cause protein inactivation or even degradation, DNA chain breakage, lipid peroxidation, etc., resulting in damage to cell structure and function, and at the same time triggers oxidative stress in the body. If it cannot be removed in time, eventually Cause oxidat...

AI Technical Summary

Problems solved by technology

However, so far, little is known about the research on the antioxidant capacity of these species. There are only a few reports on individual species such as mussels and razor clams, and the determination methods are all using the iron ion reduction antioxidant capacity analysis method (Ferric reducing antioxidant power, FRAP) after processing the sample, using a spectrophotometer to

This conventional method has several disadvantages: 1, time-consuming

The amount of sample required each time is at least 2ml; 3. Poor repeatability

Due to the long exposure time of the sample in the air, the variation between the repeated measurement values ​​of the sample is large; 4. Weak practicability

Only a single species or a small number of species can be determined each time, and batch determination is not possible, and the practicability is weak

This conventional method for determining TAC is far from being able to meet the needs of scientific research on a wide variety of marine bivalve molluscs. Therefore, a rapid method for determining TAC must be established

So far, there has not been any report on the rapid determination of the total antioxidant capacity of marine bivalve molluscs

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