Low molecular weight carrageenan oligosaccharide and preparation method and application thereof

A carrageenan oligosaccharide and low molecular weight technology, which is applied in the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc., can solve problems such as toxic and side effects, achieve weight loss, small molecular weight, and relieve lung inflammation Effect

Active Publication Date: 2012-06-27
OCEAN UNIV OF CHINA
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  • Abstract
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  • Claims
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Problems solved by technology

[0005] Aiming at the defects that influenza A virus is easy to produce drug resistance to existing drugs and most of the existing anti-influenza virus drugs have different degrees of toxic and side effects in the prior art, the purpose of the present invention is to provide a low molecular weight carrageenan oligosaccharide Its preparation method and application in anti-infection and proliferation of H1N1 subtype influenza virus

Method used

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  • Low molecular weight carrageenan oligosaccharide and preparation method and application thereof
  • Low molecular weight carrageenan oligosaccharide and preparation method and application thereof
  • Low molecular weight carrageenan oligosaccharide and preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0021] Embodiment 1: Preparation of novel carrageenan oligosaccharides

[0022] Add 0.5-1g of refined κ-carrageenan into 75ml of water, and dissolve in a water bath at 80°C to obtain a uniform solution. Then transfer the reaction vessel to a constant temperature water bath at 60°C. After the temperature of the system is stable, add 25ml of 0.4mol / L sulfuric acid solution while stirring, so that the final concentration of κ-carrageenan is 5-10mg / ml, and the final concentration of sulfuric acid 0.1mol / L, at this temperature, stir the reaction in a water bath for 3 to 4 hours; after the reaction, use BaCO 3 Neutralize the reaction solution, accurately measure the pH to 6-7, then add 0.4mol / L NaOH solution dropwise to adjust the pH to 7-8, concentrate the solution to 5ml, freeze-dry to obtain κ-carrageenan oligosaccharide (KCO).

[0023] The prepared κ-carrageenan oligosaccharide (KCO) is a low molecular weight carrageenan oligosaccharide, and its sugar chain skeleton structure i...

Embodiment 2

[0024] Example 2: Evaluation of the activity of carrageenan oligosaccharides against type A H1N1 subtype influenza virus

[0025] The obtained carrageenan oligosaccharides were evaluated for their effects on the in vivo and in vitro infection and proliferation of Influenza A H1N1 subtype influenza viruses according to the general method of testing the effect on influenza viruses in this field. The specific experiments and results are as follows.

[0026] 1. Carrageenan oligosaccharide KCO in vitro anti-replication effect of H1N1 influenza virus

[0027] Infection of dog kidney epithelial cells (MDCK) with influenza A virus mouse-adapted strain H1N1 (A / PR / 8 / 34, provided by Wuhan Institute of Virology, Chinese Academy of Sciences) was used to establish a cell model, and this cell model was combined with CPE inhibition test and virus titer Determination experiment (TCID 50 method) and MTT method to detect the inhibitory activity and cytotoxicity of carrageenan oligosaccharide KC...

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Abstract

The invention provides a low molecular weight carrageenan oligosaccharide. The low molecular weight carrageenan oligosaccharide is formed by alternative connections of 1,3-beta-D galactose and1,4-alpha-D galactose, and has a weight-average molecular weight of 1-3 kD and a structural formula of G4S-[A-G4S] <n->, wherein n is 1-7, and a sulfate radical content is 8-12%. The carrageenan oligosaccharide can not only oppose infection of Influenza A virus subtype H1N1 in vitro, but also substantially mitigate mice lung inflammation caused by virus infection, reduce mortality and prolong survival time. The carrageenan oligosaccharide of the invention is from sea red alga, has many advantages of wide source, low toxicity and high efficiency, can well inhibit infection of Influenza A virus in vitro and in vivo and has wide market application prospect in controlling Influenza A virus.

Description

technical field [0001] The invention belongs to the field of marine medicines, and in particular relates to a low-molecular-weight carrageenan oligosaccharide and its preparation method and application. Background technique [0002] Influenza A Virus (IAV) belongs to the Orthomyxoviridae family (Orthomyxoviridae), which is a negative-strand RNA virus with an envelope; According to the structure and gene characteristics, it can be divided into several subtypes, such as H1N1, H5N1 and so on. Influenza A virus (IAV) has caused at least three large-scale influenza outbreaks in the last century, the most serious of which was the Spanish flu outbreak in 1918-1919, which caused more than 40 million deaths (Lamb et al., Nat. Med. , 2001, 7: 1286-1288). Since April 2009, the H1N1 subtype influenza A virus originating in Mexico has caused worldwide influenza outbreaks, posing a huge threat to human health. According to the statistics of the World Health Organization, about 600 mill...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H11/00C07H1/00C08B37/00A61P31/16A61P11/00
Inventor 王伟管华诗李春霞张攀
Owner OCEAN UNIV OF CHINA
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