Method for catalytic synthesis of arbutin by using lipase

An arbutin and lipase technology, applied in the field of biocatalysis, can solve the problems of harsh reaction conditions, low enzyme activity and high cost, and achieve the effects of mild reaction conditions, simple process steps and long service life

Inactive Publication Date: 2012-06-27
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical synthesis of arbutin has complex process steps, harsh reaction conditions, and the use of a large amount of toxic solvents, causing serious pollution to the environment
[0005] In order to overcome these problems, many studies have begun to use enzymatic methods to catalyze the synthesis of arbutin. However, the commonly used glycosidase has the disadvantages of high cost and low enzyme activity, which is not conducive to industrialization.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] (a) Synthesis of arbutin catalyzed by free lipase

[0046] Add 0.11g (1mmol) of hydroquinone and 0.18g (1mmol) of glucose into 10ml of tert-butanol, and react with 0.02g of free porcine pancreatic lipase as a catalyst at a temperature of 40°C and a shaking table speed of 150r / min. Under the conditions, the reaction was 24 hours, and the conversion rate reached 65%;

[0047] (b) Recovery and multiple use of free lipase

[0048]After the reaction is over, the free lipase can be recovered by centrifuging the reaction solution at 6000r / min for 15min, and the recovered lipase is put into 10ml tert-butanol containing 0.11g (1mmol) hydroquinone and 0.18g (1mmol) glucose to react again Continue the reaction in the system; repeat the cycle so that free lipase catalyzes the synthesis of arbutin multiple times. Under this condition, the free lipase is transformed for 8 cycles, and the conversion rates of arbutin are: 64.5%, 63.0%, 63.0% %, 62.2%, 60.0%, 58.5%, 55.0%, 52.6%, so t...

Embodiment 2

[0055] (a) Synthesis of arbutin catalyzed by free lipase

[0056] Add 0.11g (1mmol) of hydroquinone and 0.36g (2mmol) of glucose into 10ml of 10% tert-butanol containing dimethyl sulfoxide, and react with 0.05g of free porcine pancreatic lipase as a catalyst , under the conditions of a temperature of 40°C and a shaker speed of 150r / min, the reaction was carried out for 72 hours, and the conversion rate reached 85%;

[0057] (b) Recovery and multiple use of free lipase

[0058] After the reaction is over, the free lipase can be recovered by centrifuging the reaction solution at 6000r / min for 15min, and the recovered lipase is put into 10ml dimethyl-containing solution containing 0.11g (1mmol) hydroquinone and 0.36g (2mmol) glucose again. The sulfoxide volume fraction is 10% tert-butanol and continues to react; Repeat the cycle like this, realize free lipase repeatedly catalyzes the synthesis of arbutin, under this condition, free lipase transforms 9 cycles, the transformation ...

Embodiment 3

[0062] (a) Synthesis of arbutin catalyzed by free lipase

[0063] Add 0.11g (1mmol) of hydroquinone and 0.90g (5mmol) of glucose into 10ml of 10% tert-butanol containing dimethyl sulfoxide by volume, and react with 0.2g of free porcine pancreatic lipase as a catalyst , under the conditions of a temperature of 40°C and a shaker speed of 150r / min, the reaction was carried out for 72 hours, and the conversion rate reached 96%;

[0064] (b) Recovery and multiple use of free lipase

[0065] After the reaction is over, the free lipase can be recovered by centrifuging the reaction solution at 6000r / min for 15min, and the recovered lipase is put into 10ml dimethyl-containing solution containing 0.11g (1mmol) hydroquinone and 0.90g (5mmol) glucose again. The reaction is continued in 10% tert-butanol by volume of sulfoxide; the cycle is repeated so that free lipase catalyzes the synthesis of arbutin multiple times. Under this condition, free lipase converts 10 cycles, and the conversio...

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Abstract

The invention relates to a method for catalytic synthesis of arbutin by using dissociative or immobilized Porcine pancreatic lipase. The method comprises the process steps of selection of a reaction system, preparation of immobilized lipase, optimization of reaction conditions, extraction of a product, etc. According to the invention, hydroquinone and sugar mixed according to a mol ratio of 1: 1 to 1: 5 are added into a reaction medium, and lipase is used as a catalyst for a reaction; the reaction lasts for 10 to 120 h at a temperature of 20 to 60 DEG C, and a conversion rate of arbutin is as high as more than 95%; and the usage amount of lipase is 0.01 to 5 times of the weight of sugar. Arbutin is synthesized under the catalysis of dissociative or immobilized lipase in the method for the first time, and the method has the advantages of mild reaction conditions, repeated usability of lipase, a short production period, a simple extraction process for the product, etc., thereby enabling production cost to be substantially reduced.

Description

technical field [0001] The invention belongs to the field of biocatalysis, and relates to a preparation method for catalyzing and synthesizing arbutin by free or immobilized porcine pancreatic lipase. Background technique [0002] Arbutin can significantly inhibit tyrosinase activity, reduce the deposition of tyrosinase in the skin, and have the effects of bleaching, preventing discoloration and freckle removal on the skin; it is an internationally recognized efficient and safe whitening that is being promoted It is an ideal additive for whitening, freckle removing and sunscreen cosmetics. [0003] Enzyme immobilization (Enzyme immobilization) technology is a new biotechnology developed in the 1960s, and its practical application effect is remarkable in chemical industry, fermentation production, energy, medicine and other industries. It is a method of using physical or chemical means to locate the free enzyme in a limited space area and keep it active for repeated use. Co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/44
Inventor 邓利刘春巧王芳谭天伟聂开立曹熙
Owner BEIJING UNIV OF CHEM TECH
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