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Method for preparing total elecampane sesquiterpene lactone extract

A technology of sesquiterpene lactone and inulin, which is applied in the field of medicine, can solve the problems of high solvent toxicity, long extraction cycle, and small preparation amount, and achieve the effects of high content, high yield, and stable process

Inactive Publication Date: 2012-07-04
苏州宝泽堂医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above two methods have long extraction period, high solvent toxicity, high cost, low extraction rate of inulin sesquiterpene lactone, and small preparation amount

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Weigh 10kg of radix elixir and grind it into coarse powder, heat and reflux with 80% methanol to extract 3 times, extract for 3h, 2h, and 1.5h respectively, filter, combine the filtrate, add 2% activated carbon to reflux for decolorization for 1h, filter out the activated carbon, and decolorize the decolorized solution. The methanol was recovered under pressure until the alcohol content was lower than 10%. The concentrated solution was added with 3L of ethyl acetate, stirred thoroughly, allowed to stand for stratification, and extracted twice. The ethyl acetate extracts were combined, concentrated under reduced pressure, and dried in vacuo to obtain the total lactones of inulin. Dissolve the prepared total lactones of inulin in ethanol, mix the sample with the same amount of polyamide resin, take another polyamide resin and dry-pack it into a column, put the mixed sample of polyamide resin on the clean polyamide resin, first use the Impurities were eluted with ionized wa...

Embodiment 2

[0017] Weigh 10kg of radix radix and crush it into a coarse powder, heat and reflux with 90% methanol to extract twice, extract for 3 hours and 1.5 hours respectively, filter, combine the filtrates, add 3% activated carbon to reflux for 1 hour for decolorization, filter out the activated carbon, and recover the decolorized solution under reduced pressure Methanol until the alcohol content is lower than 10%, add 3L ethyl acetate to the concentrated solution, stir well, let stand to separate layers, extract 3 times, combine the ethyl acetate extracts, concentrate under reduced pressure, and dry in vacuo to obtain the total lactones of inulin. Dissolve the prepared total lactones of inulin in ethanol, mix the sample with the same amount of polyamide resin, take another polyamide resin and dry-pack it into a column, put the mixed sample of polyamide resin on the clean polyamide resin, first use the Impurities were eluted with ionized water, then eluted with 20% ethanol, and then el...

Embodiment 3

[0019] Weigh 10kg of radix elixir and pulverize it into a coarse powder, heat and reflux with 70% acetone to extract twice, extract respectively for 2.5h and 1.5h, filter, combine the filtrate, add 1% activated carbon to reflux for decolorization for 1.5h, filter out the activated carbon, and decolorize the decolorized solution Recover methanol under pressure until the alcohol content is less than 10%, add 3L of diethyl ether to the concentrated solution, stir thoroughly, let stand to separate layers, extract twice, combine the diethyl ether extracts, concentrate under reduced pressure, and dry in vacuo to obtain the total lactones of inulin. Dissolve the prepared total lactones of inulin in ethanol, mix the sample with the same amount of polyamide resin, take another polyamide resin and dry-pack it into a column, put the mixed sample of polyamide resin on the clean polyamide resin, first use the Impurities were eluted with ionized water, then eluted with 20% ethanol, then elut...

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PUM

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Abstract

The invention provides a method for preparing total elecampane sesquiterpene lactone extract. The method comprises the following steps: smashing dried elecampane roots, extracting solution through polar organic solvent, adding 1-3% of activated carbon to decolorize extracted solution, performing filtering and pressure reduction to recover reagent, concentrating the reagent to have alcohol content lower than 10%, using nonpolar organic solvent to perform extraction and obtain total elecampane lactone, performing polyamide resin column chromatographic separation to collect required ingredients and obtaining the total elecampane sesquiterpene lactone extract. The total elecampane sesquiterpene lactone extract contains elecampane lactone and isoalantolactone lactone, preparation process yield is high, active ingredients are clear and definite, and the total elecampane sesquiterpene lactone extract is suitable for industrialized production.

Description

Technical field: [0001] The invention belongs to the technical field of medicine, and in particular relates to a preparation method of an extract of total sesquiterpene lactones of inulin. Background technique: [0002] Inula helenium L. is the dry root of Inula helenium L., a plant of Compositae. It tastes pungent, bitter, warm in nature, and returns to the liver and spleen meridians. This traditional Chinese medicine was included in the 2005 and 2010 editions of "Chinese Pharmacopoeia". Pain, restless fetus". Tibetan medicine uses the roots of Inula racemosa Hook.f. and Inula racemosa Hook.f. as medicine to treat diseases such as wind-heat syndrome, blood fever and Bacon's disease. [0003] The root of inulin contains 1-3% volatile oil, and the main components in the oil are inulin, different inulin, dihydroinulin and the like. Modern studies have shown that the main active ingredients in inulin are sesquiterpenoids, and this type of compound has received more and more ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28A61P31/04A61P31/06A61P31/10A61P33/14A61P35/00A61P35/02A61K125/00
Inventor 李法庆刘东锋
Owner 苏州宝泽堂医药科技有限公司
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