Heat-resistant freeze-dried protective agent for swine fever spleen and lymph tissue origin live vaccine and preparation method for heat-resistant freeze-dried protective agent

Abstract

The invention discloses a heat-resistant freeze-dried protective agent for a swine fever spleen and lymph tissue origin live vaccine. A solution is prepared by using water for injection as a solvent and comprises the following components in percentage by mass: 3 percent of gelatin, 5 percent of polyvinylpyrrolidone, 1 percent of fructosan, 3 percent of trehalose, 3 percent of glycine, 3 percent of sorbierite and 2 percent of sodium glutamate which are dry substances. The preparation method comprises the following steps of: (1) dissolving the gelatin and the polyvinylpyrrolidone (K90) which can be sterilized under high pressure into the water for injection according to the ratio and sterilizing at the temperature of 121 DEG C for 15 minutes; (2) dissolving the sorbierite, the sodium glutamate, the fructosan, the glycine and the trehalose which cannot resist high temperature into the water for injection according to the ratio and filtering and sterilizing by using a filter membrane with the aperture of 0.22 mu m; and (3) mixing the solution obtained in the step (1) and the solution obtained in the step (2) to obtain the heat-resistant freeze-dried protective agent. The invention relates to a low-cost and long-acting product and a preparation method thereof, which solves the problem of high transportation and storage cost for veterinary biological products for a long time.

Description

technical field [0001] The invention relates to a heat-resistant freeze-drying protective agent and a preparation method of a live vaccine of swine fever spleen and gonorrhea, and belongs to the field of production of live virus vaccines for animals. Background technique [0002] In the field of vaccine products, lyoprotectants and their preparation processes are a key technology in the production of live vaccines, which have a great impact on the storage, transportation and use of vaccines. At present, in my country's veterinary vaccines, live freeze-dried vaccines account for a considerable proportion. The commonly used protective agents in China are mainly milk, sucrose, gelatin, etc., with simple formulations and poor protective functions. If it is stored at 2°C-8°C, the storage period is only 4-6 months, and it needs to be stored below -15°C. In addition, the development of traditional veterinary biological product protective agents in China is relatively lagging behin...

AI Technical Summary

Problems solved by technology

Wang Dong et al. (2002) have developed a heat-resistant freeze-drying protective agent. The vaccines freeze-dried with this protective agent are stored at 2°C-8°C for 24 months. Not fully promoted

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