Detection method for kidney tonifying and vigour nourishing mixture

A detection method and mixture technology, which can be applied to measurement devices, instruments, scientific instruments, etc., can solve the problem of lack of content determination of Yishenyangyuan mixture, and achieve the effects of high sensitivity, stable baseline and good separation.

Inactive Publication Date: 2012-07-04
广州白云山潘高寿药业股份有限公司
View PDF1 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The product standard is recorded in the 20th volume of traditional Chinese medicine preparations in the "Drug Standards of the Ministry of Health of the People's Republic of China". The standard number is WS3-B-3971-98. There are only three simple chemical identification items in the standard, and there is no cont

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection method for kidney tonifying and vigour nourishing mixture
  • Detection method for kidney tonifying and vigour nourishing mixture
  • Detection method for kidney tonifying and vigour nourishing mixture

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0021] Example 1 Detection method of Yishen Yangyuan mixture

[0022] HPLC method is used to determine the content of psoralen and isopsoralen in Yishen Yangyuan mixture, including the following steps:

[0023] (1) Prepare the test solution:

[0024] Preparation of sample solution: Take 25ml of Yishenyangyuan mixture and put it in a 100ml volumetric flask, add methanol to the mark, shake, and filter, take 5ml of the filtrate and place it in a 10ml volumetric flask, add mobile phase to the mark, shake it, and get it;

[0025] (2) Prepare the reference solution:

[0026] Accurately weigh the appropriate amount of psoralen and isopsoralen reference substances that have been dried under reduced pressure with phosphorus pentoxide for 24 hours, and dissolve them in methanol to a constant volume to prepare a control containing 10 μg of psoralen and isopsoralen per 1 ml. Product solution

[0027] (3) Determination

[0028] Chromatographic conditions: Chromatographic column is Agilent C18 column ...

Example Embodiment

[0034] Example 2 Conditional screening and methodological verification of a quality analysis method for Yishen Yangyuan mixture

[0035] 1. Selection of extraction solvent

[0036] The solubility of psoralen and isopsoralen in ethanol and methanol is the same, but the sample solution obtained by ethanol dilution is more viscous, impurities and sugar in the sample are more soluble in ethanol than in methanol, so methanol is used as the solvent .

[0037] 2. Selection of detection wavelength

[0038] Take the above-mentioned mixed solution of psoralen and isopsoralen reference substance and scan it on a UV spectrophotometer at a wavelength of 200nm~500nm. The result is that there is a maximum absorption peak at a wavelength of 246nm, so 246nm is used as the detection wavelength.

[0039] 3. Selection of mobile phase

[0040] When the volume ratio of methanol to water reaches 35:65, there is a good separation between the main peak and the impurity peak, and the entire analysis process can ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a detection method for a kidney tonifying and vigour nourishing mixture. According to the method, HPLC is adopted to simultaneously determine contents of two effective ingredients of psoralen and isopsoralen in the kidney tonifying and vigour nourishing mixture. According to the present invention, the method of the present invention has characteristics of high sensitivity, good resolution, stable baseline, and no interference on the negative control, and the accuracy, the reproducibility, the linear relation and the stability of the method can meet the requirements of scientific research and production.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine preparations, and in particular relates to a detection method of Yishen Yangyuan mixture. Background technique [0002] Yishen Yangyuan Mixture is a traditional Chinese medicine compound preparation composed of Polygonum multiflorum, Gouji, Psoralen, Polygonatum, Cuscuta, Rosa laevigata, Angelica, and Tangerine Peel. It is used for liver and kidney deficiency, weak spleen, sallow complexion, fatigue, poor appetite, sore waist and knees, nocturnal emission and dream release. The product standard is recorded in the 20th volume of traditional Chinese medicine preparations in the "Drug Standards of the Ministry of Health of the People's Republic of China". The standard number is WS3-B-3971-98. There are only three simple chemical identification items in the standard, and there is no content determination index. In the prior art, there is no literature report about the content determination...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N30/02
Inventor 魏大华卢其福陈世斌侯勤彭惠炫李春芳
Owner 广州白云山潘高寿药业股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap