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Detection method for kidney tonifying and vigour nourishing mixture

A detection method and mixture technology, which can be applied to measurement devices, instruments, scientific instruments, etc., can solve the problem of lack of content determination of Yishenyangyuan mixture, and achieve the effects of high sensitivity, stable baseline and good separation.

Inactive Publication Date: 2012-07-04
广州白云山潘高寿药业股份有限公司
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Problems solved by technology

The product standard is recorded in the 20th volume of traditional Chinese medicine preparations in the "Drug Standards of the Ministry of Health of the People's Republic of China". The standard number is WS3-B-3971-98. There are only three simple chemical identification items in the standard, and there is no content determination index. In the prior art, there is no literature report about the content determination of Yishen Yangyuan mixture. In order to better control the quality of the product and ensure that the medicines taken by patients are indeed effective, it is necessary to study the content determination method

Method used

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  • Detection method for kidney tonifying and vigour nourishing mixture
  • Detection method for kidney tonifying and vigour nourishing mixture
  • Detection method for kidney tonifying and vigour nourishing mixture

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Embodiment 1 A kind of detection method of Yishen Yangyuan mixture

[0022] Adopt HPLC method to measure the content of psoralen and isopsoralen in Yishen Yangyuan mixture, comprising the following steps:

[0023] (1) Preparation of the test solution:

[0024] Preparation of sample solution: take 25ml of Yishen Yangyuan Mixture, put it in a 100ml volumetric flask, add methanol to the mark, shake well, filter, take 5ml of the filtrate, put it in a 10ml volumetric flask, add mobile phase to the mark, shake well, and you get it;

[0025] (2) Preparation of reference substance solution:

[0026] Accurately weigh an appropriate amount of psoralen and isopsoralen reference substances that have been dried under reduced pressure with phosphorus pentoxide for 24 hours, dissolve in methanol to constant volume, and make a control containing 10 μg of psoralen and isopsoralen each in 1 ml product solution;

[0027] (3) Determination

[0028] Chromatographic conditions: the chrom...

Embodiment 2

[0034] Example 2 Condition screening and methodological verification of a quality analysis method for Yishen Yangyuan mixture

[0035] 1. Selection of extraction solvent

[0036] Psoralen and isopsoralen have the same solubility in ethanol and methanol, but the sample solution obtained by ethanol dilution is more viscous, impurities and sugar in the sample are more soluble in ethanol than in methanol, so methanol is used as the solvent .

[0037] 2. Selection of detection wavelength

[0038] Take the above mixed solution of psoralen and isopsoralen reference substance, and scan it on a UV spectrophotometer at a wavelength of 200nm to 500nm. The result is that there is a maximum absorption peak at a wavelength of 246nm, so 246nm is used as the detection wavelength.

[0039] 3. Selection of mobile phase

[0040] When the volume ratio of methanol to water reaches 35:65, there is a good separation between the main peak and the impurity peak, and the whole analysis process can b...

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Abstract

The invention discloses a detection method for a kidney tonifying and vigour nourishing mixture. According to the method, HPLC is adopted to simultaneously determine contents of two effective ingredients of psoralen and isopsoralen in the kidney tonifying and vigour nourishing mixture. According to the present invention, the method of the present invention has characteristics of high sensitivity, good resolution, stable baseline, and no interference on the negative control, and the accuracy, the reproducibility, the linear relation and the stability of the method can meet the requirements of scientific research and production.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine preparations, and in particular relates to a detection method of Yishen Yangyuan mixture. Background technique [0002] Yishen Yangyuan Mixture is a traditional Chinese medicine compound preparation composed of Polygonum multiflorum, Gouji, Psoralen, Polygonatum, Cuscuta, Rosa laevigata, Angelica, and Tangerine Peel. It is used for liver and kidney deficiency, weak spleen, sallow complexion, fatigue, poor appetite, sore waist and knees, nocturnal emission and dream release. The product standard is recorded in the 20th volume of traditional Chinese medicine preparations in the "Drug Standards of the Ministry of Health of the People's Republic of China". The standard number is WS3-B-3971-98. There are only three simple chemical identification items in the standard, and there is no content determination index. In the prior art, there is no literature report about the content determination...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 魏大华卢其福陈世斌侯勤彭惠炫李春芳
Owner 广州白云山潘高寿药业股份有限公司
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