Method for fast detecting content of dioscin in dioscorea zingiberensis by utilizing near infrared spectrum technology
A technology of near-infrared spectroscopy and Dioscorea scutellaria, applied in the medical field, can solve problems such as high production costs, waste of raw materials, and low efficiency, and achieve the effects of short process time, reduced operating costs, and high speed
Inactive Publication Date: 2012-07-04
HENAN UNIV OF CHINESE MEDICINE
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[0004] In view of the above situation, in order to overcome the defects of the prior art, the object of the present invention is to provide a method for quickly measuring the content of diosgenin in Dioscorea scutellari
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[0007] The specific implementation of the present invention will be described in detail below in conjunction with the examples.
[0008] Concrete steps of the present invention are as follows:
[0009] (1) Establishment of the calibration model: Collect samples of Dioscorea scutellaria, select 4 / 5 as the calibration sample set, and the rest as the verification sample set. The calibration sample set is used to establish the spectral calibration model. Scan the spectrometer, collect the original spectral data of the calibration sample set, use the TQ quantitative analysis software to select the preprocessing method and band of the original spectral data of the calibration sample set, and obtain the characteristic spectral information of the diosgenin content in the calibration sample set. At the same time, use The HPLC method is used to measure the content of diosgenin in the calibration sample set, the measured content data is combined with the characteristic spectrum informati...
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The invention relates to a method for fast detecting content of dioscin in dioscorea zingiberensis by utilizing near infrared spectrum technology, which effectively solves the problems that a traditional analysis method is complex, high in production cost, low in efficiency and plenty in raw material waste and comprises utilizing a near infrared spectrometer to scan near infrared absorbing spectrum of a calibration sample set, performing pre-treatment and spectrum area selection to obtain calibration sample set characteristic spectrum, and utilizing high performance liquid chromatography to detect index content of the calibration sample set dioscorea zingiberensis to serve as a reference value; utilizing a partial least squares method to combine the characteristic spectrum of the calibration sample set and the reference value to establish a calibration model, then substituting characteristic spectrum of a validation sample set into the calibration model, and obtaining a night-time ozone profile (NIR) predict value; comparing the NIR predict value with the content of validation sample set dioscorea zingiberensis detected through the high performance liquid chromatography (HPLC), performing validation on the calibration model, crushing a sample to be tested, scanning a near infrared spectrogram of the sample to be tested , and after performing pre-treatment and spectrum area selection, inputting the calibration model. The method for fast detecting the content of the dioscin in the dioscorea zingiberensis by utilizing the near infrared spectrum technology is short in time, fast in speed, accurate and capable of performing detection on line in real time, improving working efficiency and reducing cost.
Description
technical field [0001] The invention relates to the field of medicine, in particular to a method for quickly measuring the content of diosgenin in Dioscorea scutellaria by using near-infrared spectroscopy. Background technique [0002] Dioscorea zingiberensis C.H.Wright, also known as turmeric and fire head root, is a perennial herb of the genus Dioscorea in the family Dioscorea. The content of diosgenin (commonly known as saponin) in its rhizome is 1.1% to 16.15%, ranking first in the genus Dioscorea in the world. It is the main raw material for the synthesis of steroid hormone drugs and steroidal contraceptives. important raw materials. Diosgenin is the original saponin of diosgenin, and it is widely used in the treatment of rheumatoid arthritis, heart disease, anti-tumor, anti-aging, hemostasis, anti-inflammation, etc. The level of diosgenin content directly determines its clinical curative effect and industrial utilization value, but the detection method of diosgenin c...
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Login to view more IPC IPC(8): G01N30/02G01N21/35G01N21/3563G01N21/359
Inventor 白雁谢彩侠雷敬卫龚海燕左春芳
Owner HENAN UNIV OF CHINESE MEDICINE
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