Method for ion chromatography separation integrated pulsed amperometric detection and analysis of antiviral drug zidovudine

Abstract

The invention relates to a method for ion chromatography separation and analysis of antiviral drug zidovudine, in particular to a detection method for isocratic separation integrated pulsed amperometric detection and analysis of antiviral drug zidovudine. The method comprises the following steps of: actual sample treatment, baseline surevying and mapping, sample introduction and ion exchange, elution and electrochemical detection and analysis. The method has the beneficial effects that the antiviral drug zidovudine can be subjected to good separation and analysis, and the relative standard deviation of retention time, peak height and peak area is less than 2.0 percent; a sample is directly injected into an ion chromatography separation column for separation, and a spectrogram with high sensitivity can be obtained from zidovudine solution outputted by the chromatography separation column by an electrochemical detection pool via an electrochemical detector, so that the technical process is greatly simplified; and the method can also be used for detecting the content of the antiviral drug zidovudine in a blood sample.

Description

technical field [0001] The invention relates to an ion chromatographic separation and analysis method for the antiviral drug zidovudine, in particular to an isocratic separation integral pulse amperometry detection method for the antiviral drug zidovudine. Background technique [0002] Nucleoside reverse transcriptase inhibitors are the first safe and effective drugs for the treatment of patients infected with human immunodeficiency virus, and zidovudine is the first drug for the treatment of AIDS and AIDS-related ones. Among the many AZT analysis methods used in the detection of biological samples, most of them are high-performance liquid chromatography combined with ultraviolet light. In addition, HPLC-MS / MS and HPLC-MS are also used in detection. Spectral derivative spectrophotometry, micellar electrokinetic chromatography, high performance thin-layer chromatography, fluorescence chromatography, and immunoassays for the detection of AZT have also been reported. However, ...

AI Technical Summary

Problems solved by technology

But all LC / UV methods require a large amount of plasma, and the sensitivity is insufficient for pharmacokinetic studies of new drug formulations

The combination of LC and mass spectrometry is widely used in the detection of biological fluids. This highly sensitive method cannot be popularized in many laboratories due to the need for very complex instruments and equipment.

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