Method for cultivating Botryococcus braunii

A technology of Botrytis brauneni and cultivation method, applied in the field of algae cultivation, can solve the problems of loss of function, increase of nutrient load of water body, deterioration of water quality, etc.

Inactive Publication Date: 2012-07-11
HENAN NORMAL UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

On the other hand, the problem of water pollution is also very prominent. The nutrient load of the water body increases and accumulates sharp

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] In the process of cultivating Botrytis braziliana using fish pond aquaculture wastewater as a medium, the effects of temperature and light intensity on the cell growth of Botrytis braziliana were investigated. The growth condition optimization process is as follows: select the temperature of 15°C, 20°C, 25°C, 30°C, 35°C and the light conditions of 1000Lx, 3000Lx, 5000Lx, 7000Lx, 9000Lx, 11000Lx, and carry out the combined culture of the two conditions. The algal cells were collected after the cultivation to measure the biomass. The optimization results show that the botrytis brannica in the sewage grows well under the temperature of 20°C~30°C and the light intensity of 3000Lx~8000Lx, and the biomass reaches more than 0.5g / L. Among them, under the conditions of temperature 25°C and light intensity 5000Lx, the growth is the best, and its biomass reaches above 0.65g / L.

Embodiment 2

[0014] Addition of different qualities of NaHCO to primary treated domestic sewage 3 , respectively prepare the culture solution with the inorganic carbon concentration of 0~20mg / L, and the inoculum amount of Botrytis brachyphylla is 10%. Cultivate for 30 days under the conditions of a light-dark ratio of 13h:11h, a temperature of 25°C, and a light intensity of 5000 Lx. After the culture is over, the algal cells are collected by suction filtration, freeze-dried, and the algal powder is prepared to determine the total lipid content and total hydrocarbons. content.

[0015] The results showed that when the concentration of inorganic carbon in the sewage was 5~15mg / L, the botrytis brachyphylla grew well and had a high productivity effect, with a biomass of up to 0.6g / L, a total lipid content of up to 17.42%, and a total hydrocarbon content of The content can reach up to 34.50%;

Embodiment 3

[0017] Addition of different qualities of KNO to eutrophic lake water 3 , and the culture solution with a nitrogen concentration of 2-20 mg / L was prepared respectively, and the inoculation amount of Botrytis branchiosa was 10%. Cultivate for 30 days under the condition of light-dark ratio of 13h:11h, temperature of 25°C and light intensity of 5000 Lx. After the cultivation, the cells of Botrytis brachyphylla were collected by suction filtration, freeze-dried and prepared algae powder to measure the total lipid content and total hydrocarbon content.

[0018] The results show that when the total nitrogen concentration in the sewage is 10~20mg / L, Botrytis branici grows well and has a high productivity effect, the highest biomass can reach 0.588g / L, the highest total lipid content can reach 22.64%, and the highest total hydrocarbon content Up to 35.10%;

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Abstract

The invention relates to a method for cultivating Botryococcus braunii, which is technically characterized by including: using an inorganic carbon source, a nitrogen source and a phosphorus source additionally fed to adjust the concentration of nutrient salt in waste water; allowing the concentrations of the inorganic carbon source Na2CO3, the nitrogen source KNO3 and the phosphorus source KH2PO4 to be 5-15mg/L, 10-20mg/L and 1-2mg/L respectively; placing chlorella into culture solution at the temperature of 20 DEG C to 30 DEG C with lamination intensity 3000Lx-8000Lx and brightness darkness ratio 13h; and cultivating for 30 days with the brightness darkness ratio 11h, wherein the waste water can be domestic sewage, eutrophicating river water or lake water, and aquacultural water, and the waste water is used to cultivate high-concentration Botryococcus braunii rich in greases and hydrocarbons. By the method, the waste water is used for cultivating the botryococcus braunii with high material energy.

Description

Technical field: [0001] The invention relates to an algae culture, in particular to a method for cultivating Staphylococcus branici. Background technique: [0002] At present, the global energy shortage and environmental pollution problems have prompted the development and utilization of renewable and non-polluting new energy sources. On the other hand, the problem of water pollution is also very prominent. The nutrient load of the water body increases and accumulates sharply, which makes the eutrophication process of the water body accelerate, the water quality deteriorates rapidly, and some functions are lost. Brown Botrytis ( Botryococcus braunii ) can effectively use solar energy to fix CO through photosynthesis 2 , Convert inorganic substances into energy substances such as highly unsaturated alkanes and oils. In addition, the growth of Botrytis branici can utilize the nutrients in the water body and reduce the nutrient load in the water body. If the production capa...

Claims

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Application Information

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IPC IPC(8): A01G1/00
Inventor 马剑敏董文静靳同霞靳萍李光明杜晋立马帅
Owner HENAN NORMAL UNIV
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