Application of Paenibacillus macerans strain in dissolution of phosphorus source in soil or eutrophic water
A Bacillus, eutrophication technology, applied in the field of microorganisms, to achieve the effect of increasing the content of available phosphorus, promoting healthy growth, and preventing and controlling eutrophication
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Embodiment 1
[0038] Embodiment 1 establishes standard phosphorus curve
[0039] will KH 2 PO 4 Dry at 105°C for 4h, accurately weigh 0.2195g KH 2 PO 4 Dissolve in water and transfer it to a 1000ml volumetric flask, add 400ml of water and 5ml of concentrated sulfuric acid, and make up to 1000ml to obtain a 50mg / l phosphoric acid standard solution. Use 50mg / l phosphoric acid standard solution as the phosphorus source to prepare standard solutions of a series of concentrations, and establish a 450 The standard curve of the value and the phosphorus content in the solution, through the standard curve, the OD measured in the experiment can be determined 450 value to obtain the corresponding phosphorus content.
[0040] Among them, the determination of phosphorus content adopts phosphorous molybdenum yellow spectrophotometry, specifically:
[0041] (1) Weigh 2.4g NaOH, add water and keep stirring, make up to 100ml to make 6N NaOH solution;
[0042] Weigh 0.25g of 2,4-dinitrophenol solid, a...
Embodiment 2
[0045] Example 2 Preparation of seed liquor
[0046] The preserved Paenibacillus maculatum ZJU0902 was streaked on the NA agar plate by the three-zone streaking method, and cultured at 28°C for 24 hours; the single colony of Paenibacillus macumensis ZJU0902 was connected to the LB liquid medium, and incubated at 28°C. ℃, 160r / min shaker for 12h to make seed liquid, the bacterial concentration in the seed liquid is 10 8 CFU / ml.
Embodiment 3
[0047] Example 3 Determination of Phosphorus Solubility in Solid Medium
[0048] Add 5g / l inorganic phosphorus Ca to NBRIP liquid medium (10g glucose, 5g magnesium chloride hexahydrate, 0.25g magnesium sulfate heptahydrate, 0.2g potassium chloride, 0.1g ammonium sulfate, 1000ml distilled water, pH 7.0). 3 (PO 4 ) 2 , CaHPO 4 , FePO 4 and AlPO 4 And 5ml / l organophosphate phytic acid as a phosphorus source, and then add agar (20g / l) to prepare a solid medium. After autoclaving, the plate was poured, and the Paenibacillus macerates ZJU0902 purified and cultured in Example 2 was dipped on the plate with a sterilized toothpick, and cultured at 30°C without light. After 4 weeks, the diameter of the transparent phosphorus dissolution circle was detected. Repeat 4 times.
[0049] The results showed that Paenibacillus maculatum ZJU0902 could dissolve Ca 3 (PO 4 ) 2 , CaHPO 4 and phosphorus in phytic acid, and the maximum amount of phosphorus dissolved in phytic acid. In sol...
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