Application of Paenibacillus macerans strain in dissolution of phosphorus source in soil or eutrophic water

A Bacillus, eutrophication technology, applied in the field of microorganisms, to achieve the effect of increasing the content of available phosphorus, promoting healthy growth, and preventing and controlling eutrophication

Inactive Publication Date: 2013-12-11
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Indeed, P. macerans has been found to be involved in plant growth promotion (Timmusk et al. 1999) and pathogen suppression (Bae et al. 2000; Timmusk 2003; Li et al. 2011a,b), but not yet Report on Paenibacillus macerans as Phosphorus-Solubilizing Bacteria

Method used

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  • Application of Paenibacillus macerans strain in dissolution of phosphorus source in soil or eutrophic water
  • Application of Paenibacillus macerans strain in dissolution of phosphorus source in soil or eutrophic water
  • Application of Paenibacillus macerans strain in dissolution of phosphorus source in soil or eutrophic water

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1 establishes standard phosphorus curve

[0039] will KH 2 PO 4 Dry at 105°C for 4h, accurately weigh 0.2195g KH 2 PO 4 Dissolve in water and transfer it to a 1000ml volumetric flask, add 400ml of water and 5ml of concentrated sulfuric acid, and make up to 1000ml to obtain a 50mg / l phosphoric acid standard solution. Use 50mg / l phosphoric acid standard solution as the phosphorus source to prepare standard solutions of a series of concentrations, and establish a 450 The standard curve of the value and the phosphorus content in the solution, through the standard curve, the OD measured in the experiment can be determined 450 value to obtain the corresponding phosphorus content.

[0040] Among them, the determination of phosphorus content adopts phosphorous molybdenum yellow spectrophotometry, specifically:

[0041] (1) Weigh 2.4g NaOH, add water and keep stirring, make up to 100ml to make 6N NaOH solution;

[0042] Weigh 0.25g of 2,4-dinitrophenol solid, a...

Embodiment 2

[0045] Example 2 Preparation of seed liquor

[0046] The preserved Paenibacillus maculatum ZJU0902 was streaked on the NA agar plate by the three-zone streaking method, and cultured at 28°C for 24 hours; the single colony of Paenibacillus macumensis ZJU0902 was connected to the LB liquid medium, and incubated at 28°C. ℃, 160r / min shaker for 12h to make seed liquid, the bacterial concentration in the seed liquid is 10 8 CFU / ml.

Embodiment 3

[0047] Example 3 Determination of Phosphorus Solubility in Solid Medium

[0048] Add 5g / l inorganic phosphorus Ca to NBRIP liquid medium (10g glucose, 5g magnesium chloride hexahydrate, 0.25g magnesium sulfate heptahydrate, 0.2g potassium chloride, 0.1g ammonium sulfate, 1000ml distilled water, pH 7.0). 3 (PO 4 ) 2 , CaHPO 4 , FePO 4 and AlPO 4 And 5ml / l organophosphate phytic acid as a phosphorus source, and then add agar (20g / l) to prepare a solid medium. After autoclaving, the plate was poured, and the Paenibacillus macerates ZJU0902 purified and cultured in Example 2 was dipped on the plate with a sterilized toothpick, and cultured at 30°C without light. After 4 weeks, the diameter of the transparent phosphorus dissolution circle was detected. Repeat 4 times.

[0049] The results showed that Paenibacillus maculatum ZJU0902 could dissolve Ca 3 (PO 4 ) 2 , CaHPO 4 and phosphorus in phytic acid, and the maximum amount of phosphorus dissolved in phytic acid. In sol...

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Abstract

The invention discloses an application of a Paenibacillus macerans strain in the dissolution of phosphorus sources in soil or eutrophic water. The Paenibacillus macerans strain is Paenibacillus macerans ZJU0902 with a preservation number of CCTCC NO:M209158; the phosphorus source is at least one of inorganic phosphorus sources and organic phosphorus sources. According to the invention, Paenibacillus macerans ZJU0902 is used for the dissolution of inorganic and organic phosphorus sources in soil, and has the characteristics of stability, high efficiency, and wide spectra for phosphorus dissolution; After a microbial fertilizer prepared by mixing the bacterial strain fermentation broth with a fertilizer matrix is applied to soil, dominant microflora are formed rapidly; the fertilizer efficiency is good; the effective phosphorus content of the soil can be improved significantly; and the healthy growth of plants is promoted; the bacterial strain is used for the dissolution of inorganic and organic phosphorus sources in eutrophic water, can promote the absorption of superfluous phosphorus element in water by water plants, and thus prevent and control water eutrophication.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to the application of a strain of Paenibacillus macerans as a phosphorus source in dissolved soil or eutrophic water bodies. Background technique [0002] Plant growth requires some essential nutrients, and phosphorus is one of the main elements that can affect the healthy growth of plants. Phosphorus is not only a component of many important compounds in plants, but also participates in important metabolism in plants in various ways. However, 90% of the phosphorus in the soil exists in an insoluble state, which may be due to the fact that phosphorus in the natural soil easily interacts with Fe under acidic conditions. 3+ , Al 3+ Plasma complexation to form FePO 4 and AlPO 4 and other insoluble phosphates; 2+ ionic complexation to form Ca 3 (PO 4 ) 2 or CaHPO 4 and other inert phosphates, none of which can be directly absorbed by plants, hindering healthy plant growt...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05F11/08C05G3/00
Inventor 李斌周青石雨唐乔梅邱慧谢关林
Owner ZHEJIANG UNIV
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