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Stenotrophomonas maltophilia DS4

A oligotrophomonas and maltophilia technology, applied in the biological field, can solve the problem of strains without microbial degradation method of organic pollutants, etc., and achieve good ability to remove organic pollutants in saponin wastewater, strong growth ability, strong The effect of applying value

Inactive Publication Date: 2012-07-18
CHINA UNIV OF GEOSCIENCES (WUHAN)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, physical or chemical methods are mostly used in the treatment of saponin wastewater. In the prior art, there are no reports of microbial degradation methods and bacterial strains for degrading saponin wastewater organic pollutants.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1 Screening method of Stenotrophomonas maltophilia DS4

[0012] (1) Material preparation:

[0013] 1. The experimental wastewater and the bottom sludge were taken from the biochemical treatment system of a saponin production plant in Shiyan City, Hubei Province.

[0014] 2. Medium:

[0015] Separation medium: K 2 HPO 4 0.5g / L, MgSO 4 ·7H 2 O 0.5g / L, NH 4 C1 1.0g / L, CaCl 2 0.01g / L, KH 2 PO 4 0.5g / L, glucose 0.5g / L, pH 7.0.

[0016] Screening medium: K 2 HPO 4 0.5g / L, MgSO 4 ·7H 2 O 0.5g / L, NH 4 C1 1.0g / L, CaCl 2 0.01g / L, KH 2 PO 4 0.5g / L, dissolved in 1L saponin wastewater, pH 7.0.

[0017] LB liquid medium: peptone 10.0g / L, yeast extract 5.0g / L, sodium chloride 10.0g / L, distilled water 1000mL, pH7.0.

[0018] LB solid medium: peptone 10.0g / L, yeast extract 5.0g / L, sodium chloride 10.0g / L, distilled water 1000mL, agar 15g / L (solid), pH7.0.

[0019] LB slant medium: peptone 10.0g / L, yeast extract 5.0g / L, sodium chloride 10.0g / L, distilled water 1000mL, agar 20g / L (solid), pH7....

Embodiment 2

[0033] Example 2 Application of Stenotrophomonas maltophilia DS4

[0034] A single colony of Maltophilia Stenotrophomonas DS4 was picked and cultured in LB solid medium overnight. The cultured Maltophilia Stenotrophomonas DS4 and saponin wastewater (ie, experimental wastewater, taken from a certain city in Shiyan, Hubei Province) The volume ratio of the biochemical treatment system for wastewater discharged from saponin production plants is 0.5-2:100 inoculation, 30-35°C constant temperature cultivation, pH 6-8, and reaction for 48-72 hours.

[0035] The LB solid medium is: peptone 10.0 g, yeast extract 5.0 g, NaCl 10.0 g, distilled water 1000 mL, agar 15 g (solid), pH 7.0.

[0036] Measure the degradation efficiency of the selected microorganisms to the organic pollutants in saponin wastewater.

[0037] Pick a single colony separately, culture it overnight before separating the medium, and inoculate 1 mL of the bacterial solution into 9 mL saponin wastewater (the initial COD of deaci...

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PUM

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Abstract

The invention discloses Stenotrophomonas maltophilia DS4. The Stenotrophomonas maltophilia DS4 is preserved in China Center for Type Culture Collection, and the preservation number is CCTCC NO:M2010147. The Stenotrophomonas maltophilia DS4 has a strong growing ability and a good ability of removing organic pollutants from saponin wastewater in high-concentration sulfate ion environments; and the strain can remove chemical oxygen demand (COD) at the concentration of 2,930mg / L from the saponin wastewater within 72 hours, and the removal efficiency reaches 62.34 percent. The invention provides auseful bacterial source for degrading high-concentration organic pollutants in the saponin wastewater, broadens the application of functions of Stenotrophomonas maltophilia, and has relatively high application value.

Description

Technical field [0001] The invention belongs to the field of biotechnology and relates to a Stenotrophomonas maltophilia DS4. Background technique [0002] The wastewater produced in the production process of saponin (such as diosgenin) has high color, high acidity, high concentration of organic pollutants, high chemical oxygen demand (COD), and poor biodegradability. It is a very difficult wastewater to treat. The "Water Pollutant Discharge Standard for Saponin Industry" (GB 20425-2006) stipulates that COD emissions should be less than 300 mg / L since 2009. However, the current COD concentration of saponin hydrolyzed solution reaches 20000-40000 mg / L. The COD concentration of wastewater is 4000-12000mg / L. If it is directly discharged into the water body, the concentration of organic pollutants in the water body will increase significantly, which will cause serious pollution of rivers and lakes, which not only directly affects the living environment of people, but also causes the ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/34
Inventor 郑丹鲍建国
Owner CHINA UNIV OF GEOSCIENCES (WUHAN)
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