Porcine arterial vacuum lyophilization acellular matrix, as well as preparation method and application thereof

A technology of acellular matrix and vacuum freeze-drying, which is applied in the field of porcine aortic vacuum-freeze-dried acellular matrix and its preparation, can solve the problems that have not been reported, and achieve the effect of low cost and saving medical resources

Inactive Publication Date: 2012-07-25
SHANGHAI CHILDRENS MEDICAL CENT AFFILIATED TO SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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Problems solved by technology

But about a kind of porcine aorta vacuum freeze-dried decellularized matrix and its preparation method and application have not yet been reported

Method used

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  • Porcine arterial vacuum lyophilization acellular matrix, as well as preparation method and application thereof
  • Porcine arterial vacuum lyophilization acellular matrix, as well as preparation method and application thereof
  • Porcine arterial vacuum lyophilization acellular matrix, as well as preparation method and application thereof

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Experimental program
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Embodiment 1

[0026] 1. Experimental measurement of the lowest temperature of blood vessel pre-freezing

[0027] The exploration of vascular freeze-drying preservation technology is a key issue that needs to be solved urgently in preparation research. The present invention uses a lifting microcomputer-controlled program cooling instrument to cool blood vessels at a fixed rate, mainly to investigate the influence of different pre-freezing rates on the quality of blood vessel freeze-drying preservation, and to explore and obtain a process control parameter suitable for freeze-drying preservation of blood vessels. Process.

[0028] The purpose is to measure the lowest temperature that the blood vessel can reach when it is pre-freeze in the freeze dryer. The measured temperature is the final temperature set when the blood vessel is pre-freeze using the microcomputer-controlled program cooling device. It can be seen from the experiment that the blood vessel temperature during the drying process is a...

Embodiment 2

[0046] Example 2 Preparation method of vacuum freeze-dried acellular matrix of porcine aorta

[0047] 1. Freezing intervention treatment: using a pre-freezing rate of 1K / min and a final temperature of -42.9°C to freeze the porcine aortic vessels;

[0048] 2. Decellularization: use low concentration detergent to rehydrate the matrix while decellularizing treatment, freeze-dried blood vessels with 0.25% trypsin PBS solution (containing EDTA 0.04%) at 37°C, 5% CO 2 In the incubator for 12 hours, then the 0.5% Tritox X-100 PBS solution was shaken at room temperature for 4 hours, and the PBS solution was shaken and washed 3 times, each for 30 minutes;

[0049] 3. Use the conditions of step 1 to vacuum freeze-dry the substrate again, and store at room temperature.

[0050] The present invention takes the porcine aorta as the research object, and takes the three-dimensional construction of the blood vessel clinical transplantation as the long-term goal to perform vacuum freeze-drying, decel...

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Abstract

The invention provides a porcine arterial vacuum lyophilization acellular matrix, as well as a preparation method and application thereof. The preparation method of the porcine arterial vacuum freeze-drying acellular matrix comprises the following steps: performing vacuum lyophilization to the porcine arterial blood vessels to obtain the best pore by adopting an optimal pre-freezing velocity; rehydrating the matrix with low-concentration detergent and performing acellular treatment; and lyophilizing the matrix and storing at normal temperature. The matrix has the advantages that the porcine aorta is used as a studying object, and a lyophilized acellular blood vessel with proper porosity, mechanical performance and immunogenicity is produced by virtue of optimized preparation conditions; animal in-vivo experiments prove the practicality of three-dimensional blood vessel clinical vascular transplantation, and experimental basis is provided to further clinical application; the preparation method has extremely low cost, so that precious medical resources reach billions of RMB can be saved assuredly; and experimental basis and guidance are provided to clinical application of tissue engineering arterial blood vessels and optimizing research and development of acellular blood vessels.

Description

technical field [0001] The invention relates to the field of tissue engineering, in particular to a porcine aorta vacuum freeze-dried decellularized matrix and its preparation method and application. Background technique [0002] Every year, 150,000 children with congenital heart disease are born in my country. However, a large number of blood vessels or biological patch substitutes are required in congenital heart surgery. According to preliminary estimates, the prospect of the domestic medical market alone is about 100 million yuan per year. However, artificial blood vessels commonly used in clinical practice and cryopreserved blood vessels of the same kind are non-growth and easily cause stenosis and calcification, often requiring reoperation, which brings great pain to patients and wastes a lot of medical resources. Moreover, the commonly used artificial blood vessels or biological patches are mostly imported, and the price is extremely expensive. Since the 1980s, wit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36
Inventor 殷猛孙锟刘锦纷陶乐仁范新东董凌燕刘萌芳曹清殷秋明张海波郑景浩徐志伟
Owner SHANGHAI CHILDRENS MEDICAL CENT AFFILIATED TO SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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