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Method for preparing soybean peptide by immobilized alkaline protease

A technology of soybean polypeptide and protease, which is applied in the direction of protein food processing, plant protein processing, protein food ingredients, etc., can solve the problem of unrecoverable cost and achieve low production cost, mild reaction conditions and high quality

Inactive Publication Date: 2012-08-01
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the direct hydrolysis with protease has defects such as non-recyclability and high cost, so its industrial application is limited.

Method used

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  • Method for preparing soybean peptide by immobilized alkaline protease
  • Method for preparing soybean peptide by immobilized alkaline protease
  • Method for preparing soybean peptide by immobilized alkaline protease

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0030] Experimental example 1 Screening experiment of optimal parameters of soybean protein enzymatic hydrolysis process conditions

[0031] 1 Materials and methods

[0032] 1.1 Materials and reagents

[0033] Soybean Protein Isolate Hargaoke

[0034] protex 6L alkaline protease Danish novo company

[0035] 1.2 Main instruments and equipment

[0036]

[0037]

[0038] 1.3 Experimental method

[0039] 1.3.1 Preparation of immobilized alkaline protease

[0040] A certain mass of sodium alginate was weighed in a boric acid buffer solution with a certain pH to prepare sodium alginate solutions with different mass fractions. Measure a certain amount of protex 6L alkaline protease and dilute it with borate buffer. Mix a certain mass fraction of sodium alginate solution with protex 6L in a certain proportion, and after fully stirring evenly, use a peristaltic pump to inject a certain mass fraction of CaCl at a height of 5cm 2 Gel beads were formed in the medium and place...

experiment example 2

[0088] Experimental Example 2 Process parameter optimization experiment for hydrolyzing soybean protein isolate with immobilized alkaline protease packed bed reactor Based on the optimal immobilized alkaline protease process determined in Experimental Example 1, immobilized enzyme was prepared and the packed bed reactor was used to hydrolyze soybean protein isolate , determine the range of hydrolysis process parameters (hydrolysis temperature, pH, hydrolysis time, substrate flow rate), and use the degree of hydrolysis of soybean protein isolate as an investigation index to carry out response surface design.

[0089] 2.1 Materials and reagents

[0090] Soybean Protein Isolate Hargaoke

[0091] Alcalase alkaline protease Danish novo company

[0092] 2.2 Main instruments and equipment

[0093]

[0094] 2.3 Experimental method

[0095] 2.3.1 Packed bed reactor hydrolysis soy protein isolate method

[0096] A soybean protein isolate solution with a concentration of 1% was pr...

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Abstract

A method for preparing soybean peptide by immobilized alkaline protease belongs to soybean peptide extraction technology. The method includes steps of adding self-regulated immobilized alkaline protease into a packed bed reactor; and filling soy protein isolate liquor into the packed bed reactor to realize enzymolysis and obtaining a soybean peptide product after purification and lyophilization. Soybean protein isolates are in enzymolysis by the aid of immobilized enzyme and the bioreactor, an enzymolysis process is continuous without enzyme inactivation, and continuous production is realized. The method has the advantages of mild reaction conditions, low energy consumption, good product performance, low production cost, and the like. Besides, the immobilized enzyme can be reused, and further, the high-quality soybean peptide can be obtained.

Description

technical field [0001] The invention belongs to the field of soybean polypeptide extraction and processing, and mainly relates to a method for preparing soybean polypeptide with immobilized alkaline protease. Background technique [0002] Soybean peptide has good solubility, low viscosity at high concentration, stable to heat, no precipitation under acidic conditions, good hygroscopicity and moisture retention, and has the characteristics of promoting microbial growth and metabolism, so it can be used in the production of acidic beverages , nutritional drinks, high-protein foods, and baked foods, it has the advantages of softening food, improving flavor, and improving product quality. Using protease to directly carry out enzymatic hydrolysis reaction in vitro to prepare peptide products is a commonly used method for peptide production. The main advantages are its optimum temperature and mild pH, easy control of reaction time and reaction degree, effective control of enzymat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J3/34A23J3/16
Inventor 江连洲李杨王胜男齐宝坤王梅
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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