Method for separating and preparing high purity flavonoid glycoside compounds from actinidia valvata dunn leaves

Abstract

The present invention discloses a method for separating and preparing high purity flavonoid glycoside compounds from actinidia valvata dunn leaves. The method comprises processes of drug material extraction, crude extract preparation, and monomer compound separation and purification. The drug material extraction process, actinidia valvata dunn leaves are subjected to reflux extraction 2-4 times with ethanol with a volume concentration of 50-80%, and then the ethanol is subjected to vacuum recovering to obtain the extracting solution. In the crude extract preparation process, the extracting solution is loaded on a macroporous adsorption resin, water, ethanol with a volume concentration of 20-30%, and ethanol with a volume concentration of 50-60% are respectively adopted to carry out elution treatments, the eluent after elution with the ethanol with the volume concentration of 50-60% is collected, and is concentrated and dried to obtain the crude extract. In the monomer compound separation and purification process, a high-speed counter-current chromatography method is adopted to separate and purify the resulting crude extract, wherein the concrete steps comprise: preparing a solvent system for forming a stationary phase and a mobile phase, filling the stationary phase in a counter-current chromatography column, rotating the column, pumping the mobile phase into the column, adding the prepared crude extract to an injection valve, and receiving the target flow part according to a detection map.

Description

technical field [0001] The present invention relates to a flavonoid glycoside compound prepared from the leaves of kiwifruit opposite calyx, that is, kaempferol-3-O-α-L-rhamnosyl-(1→3)-α-L-rhamnosyl- (1→6)-β-D-galactopyranoside, kaempferol-3-O-α-L-rhamnosyl-(1→3)-(4-O-acetyl-α-L -rhamnosyl)-(1→6)-β-D-galactopyranoside and kaempferol-3-O-α-L-rhamnosyl-(1→3)-(2,4 - a process for di-O-acetyl-α-L-rhamnosyl)-(1→6)-β-D-galactopyranoside. Background technique [0002] Actinidia valvata Dunn is a plant of Actinidia genus Actinidia, mainly distributed in Anhui, Zhejiang, Jiangxi and other East China regions. Its root is cat ginseng, which is a commonly used anti-tumor traditional Chinese medicine in East China. In recent years, inventors have put a lot of energy into a series of pharmaceutical research on this medicinal plant [H.L.Xin, Y.C.Wu, Y.F.Xu, et al.Chin J Nat Med., 2010, 8: 260; H.L.Xin, X.Q.Yue, Y.F. Xu, et al.Helv.Chim.Acta, 2008, 91:575; Y.F Xu, R.L.Ge, J.Du, et al.Can...

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Problems solved by technology

[0011] The separation of the above three compounds by traditional column chromatography is time-consuming and laborious, pollutes the environment, and the fixed relative sample used will have irreversible adsorption, resulting in defects such as a decrease in the recovery rate of the sample. The technical problem to be solved by the present invention is to provide a A new fast, simple and efficient preparation process for isolating these three flavonoid glycosides from Actinidia opposite calyx leaves, to overcome the above-mentioned defects in the prior art

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