Application of apramycin sulfate to serve as litopenaeus vannamei feed additive and litopenaeus vannamei feed with apramycin sulfate
A technology of apramycin sulfate and prawn feed, which is applied in the fields of application, animal feed, animal feed, etc., can solve the problems of prolonged residual time, reduced antibiotic effect, and increased drug residue, so as to improve weight gain rate and protein efficiency , reduce the number, broad application prospects
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Embodiment 1
[0011] Add 60, 120, 180, 240 and 300 mg of apramycin sulfate per kilogram of Litopenaeus vannamei basal feed respectively as the feed for Litopenaeus vannamei of the treatment groups.
[0012] Select Litopenaeus vannamei with an initial weight of about 0.5g, and feed them with the basal diet of Litopenaeus vannamei and the feed of Litopenaeus vannamei added with apramycin sulfate. The diet of Litopenaeus vannamei was the treatment group, and the culture period was 7 weeks. The results are shown in Table 1, Table 2 and Table 3.
[0013] Table 1 Effects of apramycin sulfate on growth performance and feed utilization of Litopenaeus vannamei
[0014]
[0015] Note: different lowercase letters in the same row indicate significant differences (P<0.05), where G0 indicates the basal diet of Litopenaeus vannamei, and G60-G300 indicate the addition of apramycin sulfate 60 and 120 per kg of the basal diet of Litopenaeus vannamei, respectively. , 180, 240 and 300 mg of Litopenaeus va...
Embodiment 2
[0025] After 7 weeks of breeding, 10 Litopenaeus vannamei from each group were tested for Aeromonas hydrophila infection. The infection test was carried out by immersion in a 50L white plastic bucket with a water volume of 20L. The bacteria were cultured in a nutrient broth medium for 24 hours in a 37°C incubator, rejuvenated twice, washed with sterilized physiological saline, and collected bacteria. Centrifuge at 4000r / min for 15min, collect the precipitated bacteria, add to the water, and prepare the bacterial content of 3*10 8 cfu / mL of water. Continue to feed the original feed during the infection period, record the cumulative death of shrimp within 1h, 12h, 24h, 48h, 72h, and 96h, and calculate the survival rate and immune protection rate. The results are shown in Table 4.
[0026] Table 4: Effect of apramycin sulfate on the ability of Litopenaeus vannamei to resist Aeromonas hydrophila infection
[0027]
[0028] Note: different lowercase letters in the same column ...
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